Development and application of a novel multiplex polymerase chain reaction (PCR) assay for rapid detection of various types of staphylococci strains

Zhenbo Xu^1,2, Lin Li¹, Xihong Zhao³*, Jin Chu⁴, Bing Li¹, Lei Shi¹, Jianyu Su¹* and Mark E. Shirtliff^2,5

¹College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.

²Department of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD 21201, USA.

³Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy,

Wuhan Institute of Technology, Wuhan 430073, Hubei, China.

⁴School of Food Science and Nutrition, Faculty of Mathematics and Physical Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.

⁵Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA.