Advanced Journal of Microbiology Research

Abstract: The prevalence of nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) were studied from June 2006 – 2007 among in patients and hospital staff in a tertiary referral center setting in Ankara, Turkey. Methicillin resistance was evaluated by Kirby-Bauer disc diffusion method. Of 438 people, 106 (24.2%) were nasal carriers of S. aureus. The prevalence of nasal carriers for S. aureus were not significantly different between the hospital staff, in-patients and out-patients (p>0.05). The ove…

Abstract: A cross - sectional study was conducted in Hammer and Dasenech Districts of South Omo Zone to determine seroprevalence of caprine brucellosis and its potential risk factors. Simple random sampling method was used to select 384 adult goats with no previous history of vaccination against brucellosis in the two districts. Modified Rose Bengal Plate Test (mRBPT) and complement fixation test (CFT) were used as screening and confirmatory tests, respectively. The results revealed that 16 goats (4.2%) were found seropositi…

Abstract: The term “immunocompromised host” is generally applied to a variety of patients with various immune defects. Invasive rhinosinusitis is defined by the presence of local inflammation, with vascular and osseous necrosis with extensive soft tissue extension, and occurs almost exclusively in immunocompromised patients. Fungi have been increasingly recognized as important pathogens in severe acute and chronic sinusitis in immunosuppressed hosts. Earlier recognition of the disease, medical attention specific to the patie…

Abstract: Enterobacteriaceae were isolated from tripe purchased from five butcher shops within the Mafikeng Municipality. Samples were subjected to bacteriological examination using standard methods. Mean counts (log10 cfu/g) obtained from bleached and unbleached tripe samples for aerobic plate counts (5.62 and 7.00), Enterobacteriaceae (4.09 and 4.61), Pseudomonas (4.20 and 4.57), lactic acid bacteria (2.15 and 2.35) and Micrococci/Staphylococci (3.82 and 5.61) were noted. Enterobacteriaceae on Violet Red Bile Agar were ide…

Abstract: The aim of this research was to monitor the microbiological changes throughout the manufacture and ripening of Lighvan cheese. In summer 2009, ten independent batches of cheese were manufactured by experienced workers without any intervention by the researcher. Duplicate samples of raw milk, coagulum and 0, 15, 30, 60 and 90 days old cheese were analyzed. The main groups, such as mesophilic Lactococci, thermophilic Lactococci, mesophilic Lactobacilli, thermophilic Lactobacilli, Enterococci, and the total aerobic co…

Abstract: The nature and origin of indigenous traditional beer, makes it is prone to spoilage by a variety of microbiota in particular post-fermentation. In this study, samples of commercially and homebrewed indigenous traditional beer were collected using sterile sampling Whirl-pak® bags from local informal brewers in typical marginal urban settlements of South Africa. Both commercially and homebrewed traditional beer recorded the mean counts for total coliforms and Staphylococcus spp. circa 105 cfu.ml-1 whereas the mean TV…

Abstract: Handling, vending and hygienic quality of street foods available to local residents, internal and foreign tourists to Cape Coast, the most important tourism hub in Ghana, were investigated. Questionnaires assessed stakeholder commitment to and expectations of food hygiene. Laboratory analysis evaluated microbial contamination levels of the street foods. Patronised by both local residents and tourists, foreign tourists put the hygienic safety as the principal criterion over curiosity and price to patronise street fo…

Abstract: Human milk contains a group of bacteria with potential to be used as probiotics but the origin of these bacteria is debated. This study was aimed to identify and isolate lactobacilli in breast milk and investigate the role of consuming probiotic supplements on the lactobacilli microflora of breast milk. Ten out of twenty mothers were placed on the probiotic diet and the others were considered as controls. The breast milks of these mothers were cultured on MRS. The whole genome of 16s ribosomal DNA of different colo…

Abstract: Arbuscular mycorrhizal fungi (AMF) associations are integral, functioning parts of plant roots and are widely recognized as enhancing plant growth on severely disturbed sites, including those contaminated with heavy metals (HMs). They are reported to be present on the roots of plants growing on HM-contaminated soils and play an important role in metal tolerance and accumulation. Isolation of the indigenous and presumably stress-adapted AMF can be a potential biotechnological tool for inoculation of plants for succe…

Abstract: The aim of this study is to analyze the ability of swainsonine (SW) biodegradation by five types of transformants from the genomic library of Arthrobacter sp. HW08, explore preliminarily metabolites and deduce the metabolic pathway. Using 1000 mg·l-1 SW as the sole carbon source, MSM containing five transformants at a proportion for 1:1(V:V) was incubated at 30°C, 180 r·min-1. The degradation rate of SW was 99.78% in 48 h, and SW could be completely degraded in 72 h. Compared with samples containing SW in 0 h, two…

Abstract: This study focus on the production of antibacterial compound from Trichoderma spp. Screening of antibacterial activities in some Trichoderma spp. was investigated using CYS80 medium. Trichoderma reesei and Trichoderma viride were highly effective toward human-pathogenic bacteria tested. T. viride and T. reesei were separately applied on Sephacryl S–200 column. Column fractions No. 56 to 64 for T. viride and fractions No. 57 to 66 for T. reesei had inhibitory effect against the most pathogenic bacteria examined. T…

Abstract: The effect of an alcoholic extract of Nigella sativa seeds was surveyed on cutaneous leishmaniasis in BALB/c mice. A subcutaneous inoculation of Leishmania (L) major at the dorsal base of the tail produced swelling, inflammation, stiffness, redness and sore. As soon as symptoms appeared, the ointment was used on the wound of mice as a treatment (the seeds were dried and crushed into coarse powder. Five hundred grams of the powder were extracted with ethanol 95% v/v. The extract was concentrated under reduced pressu…

Abstract: Eighty samples of raw milk, collected from eight Tunisian centres, were characterised. All these samples contained approximately 1011 cfu/ml of mesophilic aerobic bacteria (MAB), lactic acid bacteria (LAB), yeasts and coliforms dominated the microflora of these samples. They varied from 105 to 109 cfu/ml. More than 70% of the analysed samples contained 106 cfu/ml of Pseudomonas. The content of contaminating microflora like Staphylococcus, coliforms and mesophilic and thermophilic Bacillus ranged from 102 to 109 cfu…

Abstract: To elucidate the clinical characteristics of imipenem-resistant Acinetobacter baumannii causing nosocomial infections in the First Affiliated Hospital of China Medical University, 34 isolates genotyped by pulsed-field gel electrophoresis (PFGE) were collected from January 2007 to December 2007. Isolates were most commonly found in the ICU and burn ward in this study, accounting for 47.06% (16 isolates) and 26.47% (9 isolates) of the total isolates, respectively. For the most patients infected by A. baumannii, three…

Abstract: Studies to increase the recoverability of antagonistic bacterial strains carried out from soft coral species Sinularia was cultured in a varied combination of low to high nutrient media. Each of the media was supplemented with sodium pyruvate. This study was aimed to find out whether the addition of sodium pyruvate to all solid media could significantly increase the recovery of microbes. Microorganisms on maltose amended seawater agar (2.1±0.1X104), followed by Free Lunch Medium (0.98±0.07X104) and OLIGO medium (1…