Advanced Journal of Microbiology Research

Abstract: Twenty-four strains of endophytic fungi were isolated from 50 fresh leaves samples of Ginkgo biobla L collected using the cultivation-dependent method from the campus of Anhui agricultural university in Hefei of China. Out of 24 endophytes investigated, 12 strains can produce in vitro substances that are inhibitory to all or a few of tested phytopathogens whereas the rest yielded nothing active, while all fermented broths of endophytes showed activities against one and more tested phytopathogens. These results indi…

Abstract: Ganoderma sinensis immunomodulatory protein (FIP-gsi) was a new protein in fungal immunomodulatory protein (FIP) family. Based on the recombinant FIP-gsi expressed in Escherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody. The efficacy of polyclonal antibody was detected by ELISA and Western blot. The results showed that the anti-FIP-gsi polyclonal antibody with high efficient value and specificity has been successfully preparation, and its…

Abstract: Coccidiosis causes considerable economic loss in the poultry industry. The study was designed to investigate the induced damage in renal tissue of rabbits infected with Eimeria coecicola sporulated oocysts. Animals were divided into two groups. The first group acted as the non-infected control group while the second group was infected with 50,000 E. coecicola sporulated oocysts. Infection which induced a weight loss and rabbits output were approximately 1.2 billion oocysts/g faeces on day 7 postinfection. Histologi…

Abstract: Alternaria alternata, a local isolate from fishery polluted soil, was used successfully for the biodegradation of shrimp shellfish waste in favor of the production of highly active chitinase enzyme. Chitinase production was noticeably influenced by the culture medium and the highest enzyme production was attained through the log growth phase (96 h). Pronounced increase in chitinase production was concomitant with the finest waste size. Stagnant culture conditions were more adequate than shake cultures. Statisticall…

Abstract: The performance of Holstein × Indigenous (H×I) crossbred dairy cattle in Iran was considered between 1991 and 2003. Milk, fat yield and fat percentage traits were considered in this research. Variance components were estimated using animal model (single trait) and derivative-free restricted maximum likelihood method for different traits. Crossbred animals with 50 to 70% of Holstein gene ratio have shown higher performance. The average performances for the mentioned traits were 2722.68, 122.97 and 3.97%, respectivel…

Abstract: To evaluate the impact of the antibiotic use in poultry and the development of the resistance in the gut microflora of broiler. Microbiological methods were used to investigate the susceptibility of ten lactobacilli stains isolated from digestive tract of broiler. The isolates were tested against the ten most used antibiotics in veterinary and human medicine in Algeria. The isolates exhibited high resistance to all antibiotics tested in the range 70 to 100%. The minimal inhibitory concentrations (MIC’s) also were e…

Abstract: The 11 dsRNA fragmental genome of grass carp reovirus (GCRV) is enclosed in five inner core proteins and two outer capsid proteins. The Glutathione S-transferase (GST) fusion protein expression vector pGEX-4T-3 was employed to clone and expression of GCRV outer capsid gene vp7, which was amplified by reverse transcription-polymerase chain reaction (RT - PCR) from infected Grass carp. The recombinant GST-fusion protein rVP7 was induced by 1 mM IPTG in Dh5 and confirmed by SDS-PAGE and Western blot assays using both…

Abstract: Mycoplasma agalactiae is the etiological agent of contagious agalactia that is a serious disease affecting sheep and goats. It is characterized by mastitis and subsequent failure of milk production, arthritis and keratoconjunctivitis. The aim of the study was to detection of M. agalactiae by culture and polymerase chain reaction (PCR) methods from infected Iranian goats. For detection, a total of 57 samples were taken from conjunctiva (n=11), joint exudates (n=35) and milk secretion (n=11). After enrichment in PPLO…

Abstract: The effects of nematicide application timing and type on the management of ufra disease of rice caused by Ditylenchus angustus were investigated in the rain-fed and irrigated rice ecosystems. A broad range of rice (Oryza sativa L.) genotypes were also screened for resistance to D. angustus. Ufra infestation was significantly reduced when 1 kg ai/ha furadan 5G was applied up to 20 d before transplanting of infested seedlings in the field. Three granular nematicides, sunfuran 5G, edfuran 5G and forwafuran 5G, all app…

Abstract: The diagnosis of brucellosis is the corner stone in any control and eradication program. Therefore, the main objective of the present study was to apply more advanced techniques for rapid and accurate diagnosis of brucellosis that can overcome the draw backs of the traditional diagnostic techniques. Different polymerase chain reaction (PCR) assays were applied in the present study, either singly or in a multiplex format, that enable to detect and differentiate most of Brucella species. The PCR assay detection limit…

Abstract: The occurrence of various Vibrio species in lobster hemolymph from the Persian Gulf was studied. A total number of 60 lobsters (Panulirus homarus) were caught from south coast of Iran and were studied to identify Vibrio spp. in hemolymph. Four Vibrio species including Vibrio alginolyticus, Vibrio vulnificus, Vibrio harveyi and Vibrio mimicus were identified using biochemical and molecular methods. Six lobsters (10%) contained one or more Vibrio spp. as 4 samples contained V. alginolyticus, one contained V. vulnific…

Abstract: The production and consumption of artisanal home-brewed sorghum beer (Bil-bil and Kpata) is a widespread traditional practice in the northern Sudan-Sahelian zone of Cameroon. Deoxynivanol (DON) and fumonisins B1 (FB1) in these home-brewed artisanal opaque beer samples extracted by HPLC method, before analysis by the enzyme-linked immunosorbent assays (ELISA) procedure, showed a wide range of levels. All samples were positive for DON and FB 1. DON levels varies from 140 to 730 ng/ml with a mean of 450 ±90 ng/ml for…

Abstract: The occurrence of various Vibrio species in lobster hemolymph from the Persian Gulf was studied. A total number of 60 lobsters (Panulirus homarus) were caught from south coast of Iran and were studied to identify Vibrio spp. in hemolymph. Four Vibrio species including Vibrio alginolyticus, Vibrio vulnificus, Vibrio harveyi and Vibrio mimicus were identified using biochemical and molecular methods. Six lobsters (10%) contained one or more Vibrio spp. as 4 samples contained V. alginolyticus, one contained V. vulnific…

Abstract: In many Gram-negative bacteria, including a number of pathogens such as Pectobacterium carotovorum, virulence factor production and many functions are linked to the quorum sensing (QS) systems that use diffusible N-acyl homoserine lactones (AHLs) as intercellular messenger molecules. A number of organisms also contain genes that hydrolyze AHLs into inactive products, thereby blocking the quorum-sensing systems. In this study, the diversity of bacteria that inactivate N -AHL signal in soil and potato rhizoshere was…

Abstract: Brucellosis is an infectious disease caused by different species of Brucella. The main sources of human brucellosis for general population are dairy products prepared from raw milk. The aim of this study was to determine the prevalence of Brucella abortus and Brucella melitensis in unpasteurized cheese produced in Sarab city. For this purpose, a total of 1000 cheese samples were collected from Sarab city and its rural area from September 2006 till September 2007. Samples were cultured on selective Brucella agar and…