Advanced Journal of Microbiology Research

Abstract: One hundred and twenty faecal sample of commensal Escherichia coli strains were collected from different healthy persons and tested for their susceptibility to 12 -lactam antibiotics by disc diffusion and minimum inhibitory concentration methods. Colonization with strains resistant to ampicillin (Amp), amoxicillin, carbenicillin and peperacillin was detected in 36.7% of the tested isolates. Resistance patterns to 3-6 -lactams was observed in 91.7% of the tested E. coli isolates. Transfer of Amp resistance marker by…

Abstract: A halophilic actinomycete, designated HT371T, was isolated from a soil sample collected from the shore of the salty Lake Qaroun, Egypt, and was the subject of a polyphasic study. Analysis of 16S rRNA indicated that the isolate belonged to the genus Actinopolyspora and constituted a separate clade in the Actinopolyspora 16S rRNA gene tree with similarity values of 96.5 and 96.2% with Actinopolyspora halophila DSM43834T and Actinopolyspora mortivallis DSM44261T, respectively. Isolate HT371T had chemotaxonomic and mor…

Abstract: In Saudi Arabia, water resources are largely limited to groundwater, which is used both for drinking and agriculture. There is no surface water except for a few oases and no permanent streams. The contamination of this source possibly through wastewater, agriculture activities and wild birds as well as amphibians and reptiles that have access to wells and springs. Twenty six strains of Escherichia coli, isolated from water springs in Al-Ahsa Region of Saudi Arabia were analyzed for their antimicrobial susceptibilit…

Abstract: Of the 72 endophytic bacteria isolated from healthy tomato stems and leaves from field-grown plants, the strain W4 gave strongly inhibitory effect on Botrytis cinerea Pers, with the inhibition rate 78% in dual culture assay and 100% using fermentation filtrate diluted 20 times. Based on morphological, physiological and biochemical properties, 16S rDNA gene sequences and Biolog system analysis, the isolate W4 was identified and named as Brevibacillus brevis W4. The activity determination demonstrated that the antago…

Abstract: Salinization of soil is a serious problem and is increasing steadily in many parts of the world. Recently, soil biotechnology application can improve the potential of saline soils land use in agriculture. The aim of this research was determining the salinity effect on free-living diazotroph and total bacterial populations in two saline soils. Soil samples with salinity of 35 and 70 dS/m selected. The heterotrophic and free-living diazotrophic bacterial populations were counted on nutrient agar and free-nitrogen med…

Abstract: The aim of the study was to determine the effect of the competitive period training on leukocytes, some leukocyte subgroups and C -reactive protein (CRP) in sportswomen playing in the professional handball league. The average age of the group was 20.6 ± 3.37 years, height average was 163 ± 6.30 cm, weight average was 58.73 ± 4.92 kg. Blood samples were taken before and after the training period of the competition. Subjects rested for 48 h until taking blood samples. Subsequently, leukocytes, some leukocyte subgroup…

Abstract: The anti-bacterial activity of methanol and n-hexane extracts of Moringa oleifera and Moringa stenopetala seeds was conducted on 3 bacterial species (Salmonella typhii, Vibrio cholerae and Escherichia coli) which normally cause water borne diseases. The paper disc diffusion method was used with treatments arranged in a completely randomized design and replicated four times. The highest inhibitions were observed at dilutions of 20, 5 and 40% for M. oleifera and M. stenopetala methanol extracts on E. coli, S. typhi a…

Abstract: A new high performance liquid chromatography (HPLC) method was developed based on an indirect determination of tannase activity through gallic acid measurement using paracetamol as an internal standard. The separation was performed on a C18 column using a mobile phase consisting of aqueous formic acid solution (1%) and methanol (85:15; v/v) pumped at 1 ml min-1. Samples (10 μl) were injected and signals were detected at 254 nm. Repeatability was 0.81 and 1.28 for peak normalization values of gallic acid (4.16 x 10-…

Abstract: To identify and quantitate conjunctival aerobic bacterial flora from healthy residents of three age groups in Chongqing city, Southwest China. Samples taken with moist swabs from the lower fornix of bilateral eyes of 33 children (9.85 ± 0.57 years old), 31 youths (21.23 ± 0.88 years old), and 30 elders (72.97 ± 4.94 years old) respectively were tested for bacterial cultures. Positive cultures were identified and colony forming units (CFUs) were counted. Bacterial quantity was expressed as the number of CFUs divided…

Abstract: We described construction of a novel vector, pAOX-HT, for direct cloning of polymerase chain reaction (PCR) amplified fragments and expression in Pichia pastoris. The pAOX-HT serves both as a T-vector and expression vector and can be generated from a parent plasmid by digestion with restriction enzyme XcmI. To minimize the non-recombinant background, the parent vector pAOX-HT-BSK was designed to contain a large insert. The cloning efficiency was above 95% when tested with PCR products. The linearized pAOX-HT was en…

Abstract: Pollution caused by animal wastes has become a great problem in many countries. The objective of this study was to test the effects of water potential on survival of fecal coliforms in a soil treated with 3 manures. A semiarid soil was treated with raw cow and poultry manures (CM and PM) and sewage sludge (SS) at a rate of 20 g kg-1 (dry weight basis). Three water potentials established for soil incubation were: Saturation (SAT, 0 bars), field capacity (FC, -0.3 bars), and permanent wilting point (PWP, - 15 bars)…

Abstract: To evaluate the fibrolytic potential to degrade forages, extracts from spent compost of Agaricus bisporus (SCAB) were evaluated in vitro. Firstly, SCAB was cultivated for 50, 60 and 90 days (culture stage) and enzyme activity was evaluated. The highest cellulase activity was at 60 days; xylanase activity was not affected by culture stage and laccase activity increased as culture stage increased. Secondly, extracts of SCAB cultivated for 60 days were preserved by refrigeration, freezing, freeze-drying, refrigeration…

Abstract: In this study, the presence of Campylobacter jejuni in water lines of commercial broiler house and its role in the epidemiology of the infection of broiler flocks was investigated. The study was done in three sequential commercial broiler flocks previously known to be infected with C. jejuni in two poultry houses with different water sources. C. jejuni was identified in drinking water and drinking nipple swab samples in water-line samples from both houses. Fresh fecal dropping samples were taken from broiler flocks…

Abstract: The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone and teeth mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE after cloning its cDNA from the cDNA library of a human brain cDNA library. Using this anti-body, we analyzed the distribution of MEPE in dog dental germ tissue by immunohistochem…

Abstract: Leishmaniasis is still a problem for many countries including developed ones. The subgroups of the serum complements have significant roles on the onset of the infection. The aim of this study was to determine the serum complement (C1, C3, C4) levels of cases with definite diagnosis of leishmaniasis and compare them with healthy controls. The study group included 43 visceral leishmaniasis (VL) and 13 cutaneous leishmaniasis (CL) cases. Two control groups were formed for the study. The first control group included 5…