Advanced Journal of Microbiology Research

Abstract: Oleate- 12 desaturase (FAD2) is a key enzyme involved in the conversion of oleic acid (C18:1) into linoleic acid (C18:2). Brassica napus FAD2 gene was targeted for silencing by its RNAi gene under the control of a seed-specific napin promoter. This study aims to identify the transgenic plants, and analyze the level of BnFAD2 transcripts in addition to fatty acid profile in T3 seeds. As indicated by PCR and southern blotting analysis, a total of six transgenic plants were developed. Analysis performed by RT-PCR reve…

Abstract: The objective of this study was to ascertain the nationwide prevalence and antimicrobial resistance rates of Salmonella spp. and Escherichia coli amongst domesticated finisher pigs. Fecal samples (n=840) were collected at 84 slaughterhouses in Korea in May 2009. Salmonella spp. was isolated from 21 of the 840 samples (2.5%), and comprised the following isolated serotypes: Salmonella rissen, Salmonella derby, Salmonella typhimurium. Antimicrobial susceptibility testing was performed for eight antimicrobials. Salmone…

Abstract: Heavy metals pollution represents an important environmental problem. One of these metals is mercury. The aim of this research was isolating bacteria highly resistant to mercury from dental clinic effluent to investigate their growth potential in the presence of other heavy metals, such as Zn, Cu, Ni, Ag, Cd and Pb. Three dental wastewater samples were selected and their mercury concentrations, pH, EC, BOD5 and COD were determined. The mercury-resistant bacteria were found to belong to the genera of Pseudomonas, Pr…

Abstract: The main objective of this study was to find the incidence of urogenital mycoplasma such as Mycoplasma hominis and Ureaplasma urealyticum among patients with history of infertility of unexplained origin and to ascertain their implications in non specific cervicitis and non specific vaginitis. The total number of (n = 337) female patients with history of un- explained vaginitis and cervicitis were screened to observe the prevalence of urogenital mycoplasmas in the disease. Both the high vaginal swabs (HVS) and mid-s…

Abstract: In Mexico in the region of Nopaltepec in Mexico State, the edible Cactus crop, Opuntia ficus-indica is mainly cultivated for prickly pear fruit production. This crop has problems with common pests (insects, mollusks and weeds) which may serve as reservoirs and together with the named Cactus with phytoplasma-like symptoms which through the time inhibit the fruit production, and for this reason, the farmers called these Opuntia plants as Planta Macho (male plant). For molecular identification of the probably involved…

Abstract: Yellow alkaline noodle (YAN) is very susceptible to spoilage and has a short shelf life due to the high moisture content. This study was conducted to isolate and identify spoilage bacteria of YAN in an attempt to apply local plant extracts that possess antibacterial activity in extending the shelf life of YAN. Thirty colonies were isolated from spoiled YAN and were identified using the Biolog GEN III system. Eight bacteria which consisted of five Gram-positive (GP) and three Gram-negative (GN) (Bacillus pumilus, Cl…

Abstract: The study is planned to evaluate the hygienic quality of mutton intended for export from Elkadaro slaughter house on basis of surface bacterial contamination. Seventy five samples were collected during five visits, 15 samples in each visit (five samples for each: slaughter hall, slaughter house chiller and the refrigerated vehicle at Airport after unloading). A metallic triangle was used as a template from which swabs were taken. The results of the bacterial counts revealed relatively high counts (ranges between 1…

Abstract: Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores of these agents on locally available substrates (Water Hyacinth, powdered paddy rice chaff, wheat semolina) was assessed with a view to mass production. The C. malorum isolate sporulated best on Water Hyacinth (4.08 × 107 spores ml-1), followed by wheat (1.06 ×107 spores ml-1), whereas A. jacinthicola produced more spore…

Abstract: A higher yield of ethyl acetate yeast Y1 was selected from Daqu and Xiaoqu, and the production volume of ethyl acetate was 2.152 g/l when cultured in corn saccharification mash. The strain is identified as Hansenula anomala. Then, through the single factor test to study the effects of diffident culture mediums and diffident culture methods on ester-producing, the results showed that the best ester-producing culture medium for Y1 yeast was the wheat saccharification mash. The ester yield of static culture was 40% hi…

Abstract: Di-n-butyl phthalate (DBP), one of the most popular phthalic acid esters (PAEs), is commonly found in wastewater treatment plant. In this study, a bacterial strain capable of using DBP as sole carbon and energy source was isolated from activated sludge. This strain was identified as Diaphorobacter sp., designated as QH-6, based on the 16S rRNA and gyrB gene sequence analysis. For the first time, we studied the biodegradation ability of PAEs by genus Diaphorobacter. The high performance liquid chromatography (HPLC)…

Abstract: In this study, pulsed field gel electrophoresis (PFGE) was used for genomic DNA finger printings of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Thirty strains of S. aureus collected from major hospital laboratories and public health centers, Riyadh, King Saudi Arabia were tested phenotypically by conventional methods and genotypically by multiplex-PCR for direct detection of S. aureus 16S rRNA and mecA genes. The chromosomal DNA of the isolates was examined by using pulsed-field gel electrophoresis…

Abstract: Amylase activity was detected in the culture medium of marine Wangia sp. C52, which was isolated from the Southern Okinawa Trough deep-sea sediment. In the present study, a cold-active amylase was purified to homogeneity from the culture broth by ammonium sulfate precipitation and gel filtration chromatography. The molecular mass of the amyalse was 58 kDa, and its isoelectric point was close to 5.6. The optimal pH and temperature were 30°C and 6.0, respectively. In the presence of Ca2+ and Co2+, the enzyme activity…

Abstract: Local herbalists in Nigeria claimed that extracts of certain parts, that is, stem and seeds obtained from Crinum glaucum,Treculia africana, Erythrina mildbaedi, Ficus thonningii and Xylopis aethiopica are used to treat tuberculosis patients. We conducted a laboratory-based study of this claim scientifically using the procedures employed by the local herbalists. The Mycobacterium tuberculosis strain used in this study was obtained locally and characterized based on cultural and biochemical tests on isolates of bacte…

Abstract: Staphylococcus aureus commonly causes bacterial infections such as boils, carbuncles, infections wounds, deep abscesses and bloodstream infections (or bacteraemia). Nowadays, it becomes more difficult to treat such infections due to the appearance of resistant strain known as methicillin-resistant Staphylococcus aureus (MRSA). The objective of this study was to screen on methanolic extracts of seaweed (Gracilaria changii) and local sea cucumber (Stichopus badionotus) for their anti-MRSA activities in vitro and in v…

Abstract: Studies on yeasts exposed non-steroidal anti-inflammatory drugs (NSAIDs) such as acetylsalicylic acid (aspirin) as potential anti-mitochondrial antifungals. In this study, various NSAIDs were tested for antifungal activity on the human yeast pathogens Candida albicans and Cryptococcus neoformans. Our results suggest a dual action for these drugs, that is, antifungal as well as anti-inflammatory. These results could be useful in the treatment of fungal infections.[...]