Advanced Journal of Microbiology Research

Abstract: Bacteriuria in patients with indwelling urethral catheter in Owerri Municipality of Imo State, Nigeria was studied. A total of 500 early mornings mid stream urine samples from patients admitted in different hospitals within the Municipality. The samples were microbiologically analyzed using cultural methods on different isolation media. A total of 220 isolates were identified. Identification tests showed the presence of Escherichia coli (10%), Staphylococcus aureus (9.0%), Klebsiella sp. (7.0%), Proteus sp. (5.8%)…

Abstract: The efficiency of five methanolic plant extracts obtained from Euclayptus globolus, Olea europaea, Salvadora persica, Thymus vulgaris and Ziziphus spina-christi were investigated for their inhibitory effect on the growth and aflatoxin B1 production of the toxigenic Aspergillus flavus strain isolated from wheat seeds. Extract of T. vulgaris showed the highest antifungal and antiaflatoxigenic activities as aflatoxin B1 production was absolutely arrested at 5 mg/ml, while the mycelial growth of A. flavus was completel…

Abstract: The viability maintenance of Lactobacillus paracasei IL2 and Lactobacillus plantarum IL3 strains in gastrointestinal conditions represents one of the most important characteristics regarding their use for obtaining probiotic products. The tests were performed with a cell suspension kept in 0.5% NaCl. The influences of pepsin (3 g/l) at pH values of 1.5, 2, 2.5 and 3.0, as well as of pancreatin (1 g/l) in the presence of bile salts (1.5, 2, 3 and 5 mg/ml) were determined. The influence of casein and mucin, in a conc…

Abstract: Brucellosis is an infectious and zoonotic disease of worldwide distribution. Despite its control program, the disease is endemic in Iran and remains one of the most important public health problems. The aim of this survey was to determine the seroprovalence of brucellosis in livestock animals in Sarab City, Iran. A total of 1500 animals (600 cattle, 740 sheep and 160 goats) were examined for brucellosis from February 2007 to September 2008. The examined animals were divided into two sex groups (male and female). Mo…

Abstract: A series of 231 samples of bean plants affected by bean root rot were collected from different areas of Rwanda in order to characterize the causal agents. The collected samples were used to isolate 96 typical Pythium colonies which were classified into 16 Pythium species according to their respective molecular sequences of the ribosomal ITS fragments. Inoculation assays carried out on a set of 10 bean varieties revealed that all identified species were pathogenic on common bean. However, the bean varieties used in…

Abstract: The aqueous ethanol and acetone-ethanolic extracts of Vernonia amygdalina leaves and stems were investigated for their antimicrobial activities compared to ciprofloxacin, against Escherichia coli, Klebsiella sp and Staphylococcus sp. The antimicrobial activity of each extract on the isolates was determined by agar well diffusion and minimum inhibitory concentration methods. The acetone-ethanolic extract of the leaf showed the highest antibacterial activity on Klebsiella sp in both methods used, followed by the etha…

Abstract: Ringworm is a fungal and zoonotic infectious disease, caused by different species of dermatophytes. Lesions of ringworm are usually found on the head, muzzle, ears, neck, and particularly, around the eyes of the infected animals. This study was conducted to determine the prevalence of cattle ringworm in native farms of Sarab city in Iran. A total number of 1150 cattle in native dairy farms of Sarab city were examined in this study from July 2007 till June 2008. The animals were classified into two age groups of les…

Abstract: In the present study, the phylogenetic analysis of the Streptomyces strain Eri11 isolated from the rhizosphere of Rhizoma Curcumae Longae and the antioxidant activity of the broth cultured with Eri11 were investigated. Analysis of 16S rDNA gene sequences demonstrated that the strains Eri11 was most closely related to representatives of the genera Streptomyces. The total phenols and flavonoids contents in cultured broth were detected to be13.59 ± 0.17 mg gallic acid equivalent/g and 9.93 ± 0.83 mg rutin equivalent/g…

Abstract: Corn cob hydrolysis with 1% (v/v) of sulfuric acid yielded 36.49 total reducing sugars (g/l) along with various fermentation inhibitors such as fufural, phenolics compounds and acetic acid. The acid hydrolysate detoxified with overliming plus activated charcoal brought about a maximum decrease in fufural (100%), acetic acid (62.4%) and phenolics compounds (96.6%). Treatment of hydrolysate with overliming caused 99, 51.4 and 16.7% loss in fufural, acetic acid and phenolics compounds, respectively. Fermentation by Ca…

Abstract: Eighty calf blood samples were collected from different areas of China and were examined. Sixteen non cytopathic isolates of bovine viral diarrhea virus genotype 2 (BVDV-2) were isolated by specific BVDV-2-directed RT-PCR and sandwich enzyme-linked immunosorbent assay (ELISA). The results were verified by the indirect immunofluorescence assay and the ultra-thin sections. Genes of 5'-UTR, E2 and Npro from all the 16 field isolates were sequenced. The sequence identities at the nucleotide and amino acid levels were h…

Abstract: Antimicrobial properties of Berlina grandiflora Hutch. and Dalz. leaf extract were investigated against both clinical and laboratory isolates. Susceptibility of these isolates to the extract was determined using disc diffusion method. The antimicrobial screening had wide range of activity on Escherichia coli, Stapylococcus aureus and Streptococcus spp. The diameter of zone of inhibition by the extract was 7, 8.7 and 9 mm respectively. The minimum inhibitory concentrations (MIC) were 32.81, 19.38 and 11.72 µg/ml for…

Abstract: We have developed a recombinant Salmonella typhi Ty21a vaccine candidate (rSTvacIII) displaying a multi-epitopes mycobacterial antigen called the VacIII (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoS1, Hsp 16.3 and MTB 8.4 genes) on the surface of S. typhi Ty21a (Ty21a) using PgsA protein as a carrier. The expression of VacIII protein on the surface of Ty21a was verified by western blot. Immunization in BALB/c mice with rSTvacIII induced strong Th1-type responses based on high level…

Abstract: Recent pandemics caused by virus like influenzavirus (H1N1, H5N1) reaffirm the importance of studies aiming at obtaining new virucidal and/or antiviral substances, once its prolonged use can lead to resistance to the active principles. Green propolis, which has several scientifically proven bioactive properties, was evaluated in this study as an ethanol extract regarding its virucidal capacity against avipoxvirus (APV) inoculated in chorioallantoic membrane of chicken embryos (CAM). Eggs inoculated with virus and 2…

Abstract: The aim of this study was to determine the the prevalence of in vitro resistance amongst Candida species isolated from the oral cavity of denture wearers. The in vitro susceptibility of 156 Candida isolates to amphotericin B, fluconazole, 5- fluorocytosine, caspofungin and terbinafine was determined. The Clinical Laboratory Standards Institute’ (CLSI; formally National Committee for Clinical Laboratory Standards) broth microdilution method was used and MIC50 and MIC90 determined. Candida albicans, the most frequent…

Abstract: Low vegetation coverage and irrational land use are regarded as two main factors in influencing soil loss. In order to improve vegetation recovery and to adjust land use pattern on the Loess Plateau, the data of precipitation, net primary productivity of vegetation, and eco- water requirement of main crops of key counties on the Loess Plateau are used in this paper. The concepts of utilization/adaptation capability of eco-water requirement are used and plotted against mean annual precipitation in the 1960s to 1990s…