Advanced Journal of Microbiology Research

Abstract: A cross sectional study of caprine tuberculosis was conducted on randomly selected 630 goats at, Adami Tulu Agricultural Research Centre and, smallholder farmers found around the research centre from December 2005 to June 2006. Single intradermal tuberculin test, post mortem examination of suspected goats and bacteriological culture of the pathogen were carried out to confirm the pathogen. Taking the cut off value at 3.5 mm, there was significant difference (p0.05). Goats under smallholder conditions are 3.1 times…

Abstract: The preliminary phytochemical screening of extracts of Rumex vesicarius L. and Ziziphus spina-christi (L.) Willd. showed the presence of compounds that are biologically active against the two root rot pathogens Drechslera biseptata and Fusarium solani in vitro. The relative efficacy of this action, however, differed according to the extracted plant, solvent used, extract concentration, the target fungus and phase of growth. Ethanolic extract ranked first, followed by the remaining aqueous layer fraction. Eight flav…

Abstract: Eight microsatellite loci were isolated using an enrichment protocol from Ericerus pela Chavannes, representing the first microsatellite loci available for the white wax scale. All loci were polymorphic within E. pela. Allele numbers range from six to eleven with the observed heterozygosity (HO) ranging from 0.0000 to 1.0000. These primers screened in this study provide a foundation for population genetic studies of the white wax scale.[...]

Abstract: Correctly identifying bacterial toxin is of great benefit to cell biology and medical research. In order to improve predictive accuracy, based on the concept of pseudo amino acid composition, combined with the methods of approximate entropy and IB1 algorithm, a new method is proposed to predict bacterial toxins in this paper. The improved method gives comprehensive consideration of amino acid composition, side-chain mass of the amino acid, hydrophilic, and hydrophobic characteristics of a protein sequence. The tota…

Abstract: A total of 550 subjects from the community and 190 subjects from health care-related facilities in Riyadh, Saudi Arabia were evaluated for the prevalence of nasal Staphylococcus aureus colonization and to identify risk factors associated with S. aureus and methicillin-resistant S. aureus (MRSA) colonization. Among the community subjects, 1% had nasal MRSA colonization. Subjects from health care-related facilities had a lower S. aureus colonization rate (4.2%) than community subjects (1.2%), but had a significantly…

Abstract: This experiment was designed to investigate the effects of supplementation with plant extract, either alone or in combination with an organic acid on growth performance, intestinal organ measurements and intestinal microbiology. One-day-old male Ross 308 strain broiler chicken (n=96) were allocated to 4 dietary treatments in a randomized complete block design. Dietary treatments were: (i) basal diet (as a control) (C), (ii) basal diet + organic acid mixture feed (OA), (iii) basal diet + plant extract (PE) and (iv)…

Abstract: Probiotic bacteria are used for the protection and improvement of human intestinal microflora with added health effects. In search of new probiotics, 61 lactobacilli isolates were screened for survival in acidic conditions and simulated gastrointestinal juices, tolerance to ox-gall and antimicrobial activity. Selected strains (NWS09, NWS11, NWS14, NWS19 and NWS29) were identified by sequencing their 16S rRNA gene and 16S-23S rRNA gene spacer region, and further characterized by the absence of transferable antibioti…

Abstract: This study was designed to determine the prevalence of intestinal parasites in relation to personal and environmental risk factors in Bursa province of Turkey and to compare wet mount + Lugol's iodine, formalin ethyl acetate concentration, trichrome staining and enzyme-linked immunosorbent assays (ELISA) methods used in Entamoeba histolytica/ Entamoeba dispar diagnosis. For this purpose a total of 176 faecal samples from people were randomly collected from regions of the Bursa City Centre, where crowded families of…

Abstract: The objective of this research was to determine some microbiological and chemical properties of pismaniye sweet. A total of 47 plain and with cacao pismaniye samples were purchased from retail markets in the Marmara region, Turkey. Mean moisture, ash, fat and total sugar contents were found as 3.16 to 3.31, 0.18 to 0.23, 11.67 to 11.87 and 37.10 to 35.72%, for plain and with cacao, respectively. The microbial counts were in the range of 3.05 to 3.12 log cfu g-1 for total mesophilic aerobic bacteria (TMAB). 2.19 to…

Abstract: The ability of four bacterial species (Pseudomonas sp., Bacillus sp., Klebsiella sp. and Proteus sp.) to utilize some petroleum hydrocarbons (kerosene, engine oil and automotive oil) was studied. Laboratory experiments were conducted over a five day period in Erlenmeyer flasks containing mineral salt medium (MSM) under aerobic conditions using the hydrocarbons as carbon sources. A bacteria consortium capable of utilizing the hydrocarbons was prepared from the bacterial cultures. All the bacterial species utilized t…

Abstract: To evaluate the effect of Tai Chi exercise on older people with nephritis, 124 older people with nephritis were asked to practice Tai Chi exercise for 40 min once every day. Results showed that Tai Chi” quan exercise significantly decrease high-shear whole blood viscosity, low-shear whole blood viscosity, plasma viscosity, erythrocyte sedimentation rate (ESR), hematocrit (HCT), usea nitrogen, creatinine and creatinine scavenging rate during 12 months of practice. This indicates that Tai Chi exercise is beneficial t…

Abstract: A lytic bacteriophage fTFF221, specific for nisin- producing Lactococcus lactis subsp. lactis TFF221, was first isolated from kung-jom, a Thai traditional fermented shrimp paste. The plaques were clear and round-shaped on the lawn of bacteria, indicating its lytic activity. Restriction analysis indicated that genome of phage fTFF221 was double-stranded DNA. Transmission electron microscopy revealed that it had an icosahedral head with a contractile tail and a baseplate, and might be tentatively classified as a memb…

Abstract: Aflatoxins are potent mycotoxins, which are produced by toxigenic Aspergillus species including Aspergillus flavus and Aspergillus parasiticus. The goals of this study were to characterize Aspergillus fungus isolated from rice bran and to determine the correlation between Aflatoxin B1 (AFB1) production and Aspergillus contamination in the samples. Thirty rice bran samples were collected from different regions of Mazandaran province, northern Iran. Samples containing 15 specimens were conserved for one year in the s…

Abstract: The most commonly used conventional Lowenstein Jensen (L.J) culture method requires at least 6 to 10 weeks of incubation due to the slow growth rate of the Mycobacterium tuberculosis complex. Today, there are liquid automated systems to detect mycobacteria than faster conventional LJ culture method. In this study, we aimed to compare of the VersaTrek system and BACTEC MGIT 960 system for the contamination rate and recovery of mycobacteria from clinical specimens. The recovery rates of mycobacteria were detected as…

Abstract: Shotgun liquid chromatography tandem mass spectrometry (LC -MS/MS) were employed to character the soluble proteome of male Ericerus pela cuticle at the stage of the second instar larva. A total of 278 protein groups (2584 peptides) were identified that involved in a wide spectrum of biological process and molecular function. The proteins were classified into seven categories according to their corresponding functions. Molecular weight (MW) of these identified proteins range from 11 kD to above 110 kD. (isoelectric…