Advanced Journal of Microbiology Research

Abstract: Fusarium wilt caused by Fusarium oxysporum is considered a fatal disease to bananas. Chemical control of Fusarium wilt in bananas has received little efficiency. Alternatively, biological control is a viable strategy against soil-borne diseases. The objectives of this research were to isolate antagonistic bacteria and investigate the possible mechanisms against Fusarium wilt. An antagonistic Bacillus strain, termed KY-21 against F. oxysporum f. sp. Cubense was isolated from the soil of banana’s rhizosphere by dual…

Abstract: Corn stalks were used as a support to immobilize Clostridium thermobutyricum in a fermentation process for butyric acid production. The effects of pH and acetic acid concentration on butyric acid production were examined in a steady- state 25-day continuous flow operation. A metabolic shift was induced by changing pH of the medium. The maximum and the minimum ratio of butyric acid and acetic acid produced in the fermentation process were achieved at pH 7.0 and 5.0, respectively, and the maximum yield of butyric aci…

Abstract: High ethanol yield is a desired property of industrial yeast strains. A significant problem in fermentative conversion of lignocellulosic materials to ethanol is the formation of furfural and hydroxymethylfurfural (HMF), which are formed during acid hydrolysis of lignocellulose. Furfural has been known to create strong inhibition in metabolism of the yeast Saccharomyces cerevisiae. In this article, the result of random mutagenesis of a native strain of S. cerevisiae by ultra violet (UV) light and nitrous acid is pr…

Abstract: To investigate the presence of Fusobacterium necrophorum (F. necrophorum) and Dichelobacter nodosus (D. nodosus) in dairy cattle footrot, a loop -mediated isothermal amplification method for detection of D. nodosus (Dn-LAMP) was developed and evaluated using species-specific16S rRNA gene as target gene. The Dn-LAMP showed no cross reaction with several common pathogens from cattle, and detection limit of the Dn-LAMP was 10 cfu/mL of D. nodosus. Amplicons of the Dn-LAMP could be detected by visual inspection. 189 ho…

Abstract: Recent trends in soil microbiology suggest that certain soil microbes have a positive effect on seedling growth and development. A study was conducted to investigate the interactive effect of the plant-growth promoting fungi Trichoderma harzianum and the arbuscular mycorrhizal fungi (AMF) in growth and development of tomato (Solanun lycopersicum) seedlings grown under greenhouse conditions. A 3 x 3 factorial experiment was laid out in a completely randomised design with six replications. At harvest (42 DAP), when c…

Abstract: The growth models, diameter growth rates and biomass yield of three ectomycorrhizal (ECM) fungi, Suillus tomentosus (Kauff.) Sing. Snell and Dick, Suillus laricinus (Berk.in Hook.) O. Kuntze and Aminita vaginata (Bull.: Fr.) Vitt., were investigated under water stress induced by polyethylene glycol-6000 (PEG-6000) in pure culture. The results showed that the growth models of the three ectomycorrhizal fungi were not significantly affected by water stress, but the growth rates and biomass were changed. Lower water st…

Abstract: Multidrug resistant strains of Escherichia coli are becoming a major challenge in treatment of patients with urinary tract infection. Developing effective screening methods for selection of antimicrobial resistant strains is necessary. In this study, we have compared descriptively results of E-test with Disk diffusion agar method in selection of multidrug resistant strains of E. coli among patients with urinary tract infection. In disk diffusion test, 19 of 256 urine collections were resistant to 5 antibiotics used…

Abstract: The effects of Arbuscular mycorrhizal (AM) fungi on the characteristics of leaf development of Paeonia suffruticosa at different levels of salt stress (0, 8, 16 and 24%) were studied. Potted ‘Feng Dan’ seedlings were inoculated with Glomus mosseae, and the non-inoculated was used as the control. The results showed that under salt stress leaf relative water content, sclerophyllous index of leaves and leaf succulence level of P. suffruticosa seedlings inoculated with G. mosseae were significantly higher than those of…

Abstract: Findings from a preliminary lab-scale study show strong potentials of phytodisinfectants as a low-cost, appropriate and ecological alternative technology in purifying water in rural Cameroon. A survey of plants used in water purification in Bamenda, Cameroon, indicated that there are many plants used in water treatment. A rapid screening on the coagulative and disinfection potential of four most frequently used plants was carried out on; Moringa oleifera, Jatropha curcas, calyx of Hibiscus sabdarifa, sclerotium of…

Abstract: Structural protein VP1 of foot-and-mouth disease virus (FMDV) is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The coding sequence of VP1was amplified and then identified by polymerase chain reaction (PCR) and sequencing. To achieve high-level expression of VP1 protein, we optimized VP1 gene base on Escherichia coli preferred codons and synthesized the optimized gene. The synthetical gene was cloned into the fusion expression vec…

Abstract: In this study, we aim to perform a microbiological evaluation on the anthrax cases encountered in small and large ruminants as well as in equidae in the Eastern Mediterranean region of Turkey between March 2005 and July 2010. Blood specimens from 44 animals raised in the Mediterranean region of Turkey and suspected of having anthrax in light of the clinical examination results between 2005 and 2010, were subjected to bacteriological analysis. Conventional methods such as Giemsa and Gram staining as well as culture…

Abstract: Cell-free extracts of nitrate-grown Penicillium viridicatum could catalyze the hydrolytic cleavage of N-glycosidic bond of adenosine, guanosine and inosine to the corresponding base and ribose by a ribonucleoside hydrolase; however, there is no evidence for the degradation of these compounds through phosphorylation. The rate of hydrolysis of the three ribonucleosides was in the order inosine> guanosine> adenosine. It was proven that adenosine hydrolyzing enzyme is not associated with the cell membrane. Maximum enzy…

Abstract: One pair of primers was designed based on the sequence of tmr locus for specific and sensitive detection of Agrobacterium tumefaciens. Only the A. tumefaciens strain can produce the 236bp target fragment among the fourteen bacterial species that tested. The sensitivity of the specific PCR system was determined by a nested-PCR amplification which can numbered the copies of the template DNA. According to the results, it can give positive band when only 100 copies were in the template. The protocol was carried out for…

Abstract: The study aimed to investigate the effectiveness of a novel antibiotic drug tigecycline on clinical isolates of Mycobacterium tuberculosis and, reviewing defined anti-tuberculosis effects of available agents. Minimal inhibitory concentration (MIC) of tigecycline for 50 M. tuberculosis including multidrug-resistant (MDR) clinical isolates (20 MDR isolates) was determined by broth microdilution method in the study. Tigecycline MIC values were ranging between 8 and 64 µg/ml. However, there is not any defined break poi…

Abstract: The population dynamics of a multi-species biofilm for simultaneous removal of nitric oxide (NO) and sulfur dioxide (SO2) in a laboratory-scale biotrickling-filter was described. Based on a simplified multispecies-multisubstrate model with the gas-liquid-biomembrane transfer theory, the calculations were compared to the long-term verification experiments. Simulation results were in good agreement with the experimental values of biofilm thickness, population densities of biofilm, total biomass dry weight and partial…