Advanced Journal of Microbiology Research

Abstract: The applicability of the Taguchi DOE (design of experiment) methodology for optimization of medium composition for maximum antimicrobial protein (AMP) production by Bacillus amyloliquefaciens MBL27 has been demonstrated in the present study. The influence of individual factors and the relationships between the factors and their levels were established. Three factors viz, glucose, triammonium citrate and K2HPO4, each at three levels were selected and an orthogonal array (OA) layout of L27 containing 27 well-defined…

Abstract: In this work, aromatic and taste giving herbs including thyme (n=23), basil (n=23) and mint (n=19) samples collected from retail outlets of Igdir were examined for the microbiological quality as well as the detection of some pathogenic bacteria. Total aerobic mesophilic counts varied from 102 to107 cfu/g in thyme, 103 to 107 cfu/g in basil and mint samples. Coliforms were present in 34.8% of thyme, 69.6% of basil and 36.4% of mint samples. E. coli was detected only in a mint sample. Detection rate for staphylococci…

Abstract: Crimean-congo hemorrhagic fever (CCHF) is a viral disease that can be seen in people epidemically or as single patient. CCHF is a highly deadly disease caused by Nairovirus from Bunyaviridae family. Endothelial damage caused by secreted cytokines plays an important role in the pathophysiology of the disease. Reactive oxygen species (ROS) are highly cytotoxic. Intracellular and extracellular antioxidants are the mechanisms that protect the cell from the cytotoxic effects of the ROS. In this study, malondialdehite (M…

Abstract: A strain ZHU-1 capable of utilizing chlorpyrifos as the sole carbon sources and energy was isolated from soil. ZHU-1 was identified as Bacillus licheniformis based on analysis of morphology, physiological and biochemical characters and 16S rRNA. The addition of ZHU-1 to soil treated with chlorpyrifos resulted in a higher degradation rate than noninoculated soils, the degradation rate of chlorpyrifos (100 mg kg-1) could reach 99% or above after 14 days. The microbial manure added by strain ZHU -1 can be applied not…

Abstract: The diagnosis of tuberculosis is based on the detection of Mycobacterium tuberculosis on clinical specimens with different methods. Although there are many diagnostic techniques, such as culture and molecular methods, however, sputum smear microscopy for demonstration of acid fast bacilli remains the most important diagnostic method for tuberculosis in high prevalence countries due to its speed, easy performance, and low cost. The aim of this study was determination of prevalence of acid fast bacilli (AFB) in speci…

Abstract: Cement dust pollution is one of the sources of atmospheric pollution. The main impacts of the cement activity to the environment are the broadcasts of dusts and gases. The objective of this study is to determine the effects of cement dust pollution, which was generated by cement plant, on soil microbial population, microbial respiration, and some enzyme activities in cultivated wheat (CT) and no-till (NT) soils. The fields are located at distances of 1, 3, 5, 7, 10 and 15 km away from the cement plant. In dominant…

Abstract: The expression of five innate immune-related genes crustin (MjCrus), lysozyme (MjLyz), penaeidin (MjPEN), Toll-like Receptors (MjToll), and Tumor Necrosis Factor (MjTNF) in the lymphoid organ (LO) and intestine of kuruma shrimp (Marsupenaeus japonicus) was investigated after challenge with Vibrio nigripulchritudo. Bacteria (1 x 105 CFU/ml) were injected into the second abdominal segment of the shrimp. The LO and intestine were isolated at 3, 12, 24, and 48 h post-injection, and total RNA was extracted. The expressi…

Abstract: The aim of this study was to evaluate the effectiveness of “Eight-trigram boxing” in reducing blood oxidative status and improving intestine benefical bacteria counts. Participants were randomly assigned to “Eight-trigram boxing” exercise. After 3 months, significant increase in GSH, SOD, CAT, GSH-Px and GR activities were observed. In addition, intestine benefical bacteria bifidobacterium and lactobacillus count were markedly enhanced. It could be concluded that “Eight-trigram boxing” exercise was beneficial to pr…

Abstract: In the present study, we tried to investigate whether infection with cystic echinococcosis provokes oxidative stress in the host by measuring changes in plasma levels of anti-oxidants enzymes. 23 patients and 25 control individuals were included in the study. Plasma superoxide dismutase [Cu–Zn superoxide dismutases, cytoplasmic form/superoxide dismutase 1] and glutathione peroxidase [Cytoplasmic gluthatione peroxidase 1] activities and plasma nitrite levels were all determined based on the colorimetric methods. Sta…

Abstract: The antimicrobial screening of the monoazo dyes, 4-carboxyl-2, 6-dintropheylazohydroxynapththalene, was carried out as a preliminary test for evaluating their biological potentials. Antimicrobial screening was done with filter paper disc and gauze bandage fabric impregnated with the dyes using 7 microbial isolates: Staphylococcus aureus 382, Candida albicans UBA7, Klebsiella species 864, Escherichia coli V3, Salmonella enterica gallinarum 28 V2, Pseudomonas aeruginosa and Proteus species. Diameters of zones of inhi…

Abstract: Streptomyces strains may control postharvest lemon fruit diseases caused by Penicillium digitatum and Geotrichum candidum. Four Streptomyces strains (RO3, MC3, Alem and CE1) were tested and RO3 was the strain with the highest antagonistic activity. Streptomyces RO3 showed a maximum biomass value (2.15 g/L) after 72 h incubation in yeast extract, malt extract, glucose (YMG) medium; in starch casein medium the highest value was 0.477 g/L at 120 h incubation. The metabolites produced in YMG medium showed the maximum i…

Abstract: The essential oil from the gum of Pistacia atlantica Desf. grown in Algeria was obtained by the hydrodistillation method, and its antimicrobial activities against the growth of clinical isolates of Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes were evaluated using the agar disc diffusion, the minimal inhibitory quantity (MIQ) determination method and the liquid phase by Maruzella method. The results of the study revealed that essential oil resin of P. atlantica has antimicrobial activity agains…

Abstract: The effect of high frequency Electromagnetic Induction (EMI) combined with various condition of pre-heating for inactivation of mesophil microorganisms in multilayer flexible pouches has been studied. All samples were filled in pouches and, have been put in (water bath chamber; different condition of pre heating (80°C 5 min, 80°C 10 min, 80°C 15 min, 85°C 5 min, 85°C 10 min, 85°C 15 min) have been done; and ready for EMI sterilization which discharges square-wave pulses with variable voltage 1-20 kV/cm and frequenc…

Abstract: A study was carried out to isolate hydrocarbon -degrading bacteria associated with environmental samples collected from Ilaje coastal area, Nigeria. The samples were analyzed microbiologically using standard microbiological techniques. These organisms were further studied to determine their biodegrading activities on hydrocarbons (diesel, kerosene, petrol) using enrichment medium. The microbial growths were determined using spectrophotometer blanked at 600 nm. The nine bacteria isolated from environmental samples w…

Abstract: Newcastle disease (ND) is a highly contagious infection of poultry that causes nervous signs and mortality in young ostriches. This study has been done during two years from 2008 to 2010 in Iran, in order to explore ND in ostriches died following nervous signs, and carcasses of these ostriches examined by different methods including histopathology, bacteriology and virology. No histopathological sign was found in the samples. In bacteriological study no significant bacterial agents were isolated. In virology tests…