Advanced Journal of Microbiology Research

Abstract: Isolation and identification of the drug resistant gram negative bacilli bacteria were carried out from post operative wound infections. Out of 214 samples collected, 167 showed bacterial growth and gram negative bacilli (GNB) growth was observed in 121 samples. In the present study E. coli was found to be the predominant agent isolated from wound infections (37.3%), followed by Pseudomonas (20.9%), Klebsiella (17.2%), Acinetobacter (14.2%) and other agents were less common. It was found that out of 121 gram negati…

Abstract: Culture media significantly affected the growth, sporulation and conidial discharge of any microorganisms. The present investigation was conducted to examine the effect of broth and agar media on the mycelial growth and fungal sporulation of six species belonging to five genera of fungi. Soil fungal dermatophytes (Trichophyton mentagrophytes, Microsporum gypseum and Microsporum fulvum) were isolated from soil using hair-baiting technique and clinical isolates (Epidermophyton floccosum, Trichophyton schoenleinii and…

Abstract: The worldwide spread of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) skin infections is becoming an emerging problem. These bacteria can produce virulence factors. The objectives of this study were to determine the rate of MRSA and frequency of mecA gene by PCR and detect genes eta and etb encoding exfoliatin A and B. This is a prospective study focused on school children of 5 to 15 years with a cutaneous infection from October 2007 to June 2008. The skin samples taken were allowed to is…

Abstract: Since milk is a high nutrition food, it is extremely liable to microbiological contamination. Maximum hygiene in mechanical milking and in health practices management throughout the production process is necessary so that the quality of crude milk is guaranteed. Current research comprises the collection of milk in milk-producing farms during April, May and June 2009 so that the microbiological safety of milk produced in the municipality of Amargosa BA Brazil may be verified. Results show that crude milk samples are…

Abstract: Typhoid fever remains an underestimated important health problem in many developing countries. It continues to be a global problem with an annual estimate of 1.6 million cases and 600,000 deaths. Salmonella, gram negative bacilli can survive during certain stages of host parasites interaction. There are number of drugs being used for the treatment of typhoid, but increasing occurrence of multidrug resistant (MDR) strain of Salmonella typhi has complicated its management, that thus, it has necessitated the search of…

Abstract: Methanol extract of the leaf of Heliotropium indicum was evaluated for its antimicrobial activity against five bacterial isolates comprising of four Gram-negative namely: Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp. and Proteus mirabilis; and one Gram positive, Staphylococcus aureus at 6.25, 12.5, 25, 50, 100 and 200 mg/ml including phytochemical analysis. While both S. aureus and Klebsiella spp. were inhibited at 50, 100 and 200 mg/ml with minimum inhibitory concentration (MIC) of 3 mg/ml; P. aerugino…

Abstract: Among the 100 isolates, a bacterial strain TW1 was isolated from the urine tract specimens of infected women admitted at Government hospital, Namakkal District in Tamil Nadu, India, using caprylate thallious agar medium. The organism was characterized by all biochemical tests and showed similarity with Serratia marcescens. The genomic level confirmation done with 16s rDNA primer by submitting the genomic sequence to Gene Bank under ACC.No-GU046545 after comparing, showed 98% sequence similarity with S. marcescens a…

Abstract: The horn of Africa bears the prunt of Leishmaniasis in the world where both Phlebotomus orientalis, the vector of Leishmania donovani and Phlebotomus papatasi, the vector of Leishmania major, exist. Visceral leishmaniasis (VL) caused by L. donovani is a major cause of morbidity and mortality in east Africa. The populations of east Africa are very dynamic in continuous movements between endemic areas of different leishmaniasis clinical forms. Molecular epidemiological studies have provided indirect evidence for gene…

Abstract: Investigation on the microbial safety and probiotic potentials of different yoghurt brands sold in Owerri, Imo State Nigeria, was carried out using standard microbiological procedures. Ten each, of five different brands of commercially available yoghurt packaged in plastic containers were purchased from the street vendors and shopping malls in Owerri metropolis. The mean total count of samples on Brain Heart Infusion (BHI) and De Mann Rogosa Sharpe (MRS) agar media ranged from 2.0×107 to 6.0×108 and 1.0×108 to 5.4…

Abstract: Treatment of urinary tract infections (UTIs) is becoming difficult due to the increasing trend of antibiotics resistance and this may necessitate an up to date knowledge of resistance pattern. This study was therefore set up to ascertain bacterial resistance patterns from UTIs at a University hospital. The study was retrospective in nature. Data generated from urine cultures of patients at the University of Calabar Teaching hospital for a period of five years (2004 to 2009) were compiled. Relevant information obtai…

Abstract: Recurrent secondary psoas abscess due to Pseudomonas aeruginosa is a relatively rare disease. Diagnosis is often easy through appropriate investigations but the treatment may be difficult, especially in patients with severe underlying conditions. We report a case of recurrent psoas abscess due to P. aeruginosa in same focus after osteosynthesis for kyphoscoliosis. A 65 years old man suffered in post-operative course of recurrent psoas abscess due to P. aeruginosa with relapse intervals of 6 and 2 years. As patient’…

Abstract: Staphylococcus aureus has been reported to be a major cause of community and hospital acquired infections. Indiscriminate use of antibiotics resulted in the development of multi-drug resistant S. aureus throughout the world. Development of multi-drug resistant strains of S. aureus is increasingly alarming in Bangladesh. We attempted to study the current prevalence of β-lactamase-producing and non-producing methicillin-resistant S. aureus (MRSA) in clinical samples and to find out the correlation of antimicrobial re…

Abstract: The bacteriocins produced by ENTEROCOCCUS species (enterocins) show considerable activity against diverse pathogens. One of these antimicrobial peptides was isolated from ENTEROCOCCUS FAECIUM E86 cultured in MRS broth for 24 h at 37°C. The antimicrobial peptide was purified by precipitation in 75% ammonium sulfate, followed by chloroform precipitation and chromatography in Dowex-50, resulting in a specific activity of 52.46 AU/ mg in a final yield of 80% and a 6-fold recovery. The enterocin was purified to homogene…

Abstract: Some species of “streptococci” have great role in dental caries. So control of their activities can promote prevention of dental caries. Use of herbal agents is a notable issue in recent researches. The aim of this study was evaluation of antimicrobial activity of aqueous and hydroalcoholic extract of seed and stem of Ammi visnaga against Streptococcus mutans, Streptococcus salivarius and Streptococcus sanguis. First step or screening was designed by determination of antimicrobial activity for each extracts using D…

Abstract: The antibacterial potency of ethanol, acetone and chloroform leaf extracts of Vernonia amygdalina, Ocimum gratissimum, Corchorous olitorius and Manihot palmata were screened against ten bacterial isolates using the agar-well diffusion method. The leaf extracts were screened for antibacterial activities at 25 mg/ml concentration. Antibacterial efficacy of extracts against the bacterial isolates was indicated by the appearance of clear zones of inhibition around the wells. The extracts (except that of Corchorous olit…