Advanced Journal of Microbiology Research

Abstract: This study was designed to examine the microbiological quality and semen indices of seminal fluids of sexually active males in Benin City, Nigeria, and to determine the relationship between the presence of pathogenic micro -organisms and semen parameters. Semen was collected from 229 volunteer sexually-active men, aged 19 - 33 years (mean 24.73 ± 3.4) in Benin City, Nigeria. Volunteers were advised to abstain from sex for 3 days before semen collection and also from alcohol (including other factors that may affect…

Abstract: One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65(54.2%) males and 55(45.8%) Females, with male: females ratio (1.2:1) and 60 individual without ontological problems as control group were included in this study, which was done during the period of March, 2009 to January, 2010. This Include collection of aural swab samples, culturing of samples, identification of causative agents species and antibiotic sensitivity. Gram's negative bacteria were the commonest microorganisms; it co…

Abstract: Lake Manzala is considered one of the most important coastal Lakes in Egypt. This study revealed the highly polluted condition of water and fish samples taken from three of the more important sites (Kapoty, Bashtier and Mataryia areas), representative to human activity and different ecosystems in the Lake water environment. Total suspended solids (TSS), ammonia and nitrates, of the samples taken showed figures exceeding the national and international standards. The samples from the study yielded high levels of bact…

Abstract: This is a descriptive laboratory based case study carried out in Khartoum state hospitals during the period of June, 2007 to April, 2008. The study aimed to evaluate emergence of ESBL among multi drug resistant Escherichia coli and Klebsiella species causing nosocomial UTI. Hundred strains of multi drug resistant (MDR) E. coli and Klebsiella species causing nosocomial urinary tract infections (UTIs) from two main hospitals from Khartoum (Omdurman teaching hospital and Fedail Hospital) were included in this study. S…

Abstract: Flavobacterium columnare, the etiologic agent of columnaris disease, has a broad geographical distribution and accounts for a large number of mortalities in fish species. This study aimed to generate a faster method for diagnosis of columnaris through isolation and characterization of the F. columnare 16S rDNA gene from bacteria isolated from Nile tilapia (Oreochromis niloticus), tambaqui (Colossoma macropomum) and matrinxã (Brycon amazonicus). The bacteria were characterized biochemically and by PCR-RFLP. For isol…

Abstract: Intestinal helminths are among the most common and widespread of human infections, contributing to poor nutritional status, anaemia and impaired growth. Anaemia and iron deficiency in pregnancy is a major public health problem in developing countries, but their causes are not always known. The objective of this study was to assess the prevalence and severity of anaemia and iron deficiency and their association with helminths, among pregnant women in the Ashanti region of Ghana. A cohort study was carried out in the…

Abstract: This study was to investigate the prevalence of asymptomatic bacteriuria (ASB) in patients clinically diagnosed with type 2 diabetes mellitus and to determine the antibiotic sensitivity pattern of bacterial isolates. One hundred and thirty type 2 diabetics comprising 56 males and 74 females (aged between 30- 59 years) attending the Central Hospital, Benin- City, Nigeria were studied. Mid-stream urines were collected from patients who gave informed consent aseptically into sterile McCartney bottles and examined micr…

Abstract: We provide criteria under which affinity hemodialysis could provide a stable infected equilibrium.[...]

Abstract: In the present study, twenty four Mycobacterium avium isolates obtained from clinical (sputum) and environmental (water and soil) samples were analyzed by a PCR typing method. Gel electrophoresis patterns showed that a single PCR reaction generated 21 profiles. Based on the relatedness of the PCR band patterns six isolates could be grouped in three clusters. Results have the implication that the PCR based system could be used as a simple and rapid method for molecular typing of M. avium isolates with high discrimin…

Abstract: Two hundred and seventy Bacillus species were isolated from soil samplse in Khartoum State and tested for α- amylase production. 20 potential isolates of α- amylase producer were obtained during primary screening. Secondary screening of these isolates yielded a high thermostable alkaline α-amylase producing isolate. Of all the species tested, Bacillus licheniformis gave maximum α-amylase activity of 0.7947 U/mg/ml at pH of 8 in iodine method, and (0.024 U/mg/ml) in 3.5.dinitrosalicylic acid (DNS) method. Characteri…

Abstract: Helicobacter pylori is one of the most common causes of infectious diseases around the world. Although combined antibiotic treatment for the infection exist, the development of therapies such as immunotherapy or an effective vaccine can be helpful for containment and prevention of the infection. Phage display technologies offer a simple way for selecting specific antibody fragments against a specific target. In this work, we report the construction of a phage display antibody library against H. pylori strain N2. Th…

Abstract: The present study is designed to study bacteremia and to measure some immunological parameters of diabetic patients in Kerbala City, Iraq during the period from November 2006 until May 2007. This study included a total of 125 patients with diabetes mellitus (30 type I and 95 type II), and 55 healthy persons as Control subjects. Blood samples were collected from both patients and Controls, blood culture was done for bacterial isolation and identification, virulence factors as well as antibiotic susceptibility tests…

Abstract: The objective of this study focused on the prevalence of Haemophilus influenza to confirm the colonies of H. influenza on the basis of their growth requirements and serotype distribution. This study prepared 80 isolates of H. influenze isolated from five different sources (eye, ear, sputum (SP), lower genital tract (TA), and nasopharyngeal (NPA)) with different ages for infants and elderly persons. The phenotypic characteristics, which included the biotype, serotype, antibiogram and β-lactamase production, were app…

Abstract: The IN VITRO antimicrobial susceptibility of recently isolated uropathogens was investigated using two locally produced brands of antibiotics and one imported brand, in a comparative study to determine their degree of effectiveness and the susceptibility profiles of these uropathogens. Seventy eight (78) bacterial strains containing 12 different species of both Gram negative and Gram positive bacteria were isolated and investigated. ESCHERICHIA COLI was identified as the leading cause of urinary tract infections be…

Abstract: The present investigation aimed to evaluate the efficiency of Bacillus thuringiensis β – exotoxin as a biological agent to control Musca domestica and to study its impact on some biochemical parameters of 2nd larval instar. Laboratory studies are carried by applying topically the bacterium B. thuringiensis β– exotoxin at LC30 under laboratory conditions (27 ± 2°C and 60±5% RH). The total protein contents of the total body, the total lipids, total carbohydrate, enzymatic activities of carbohydrate hydrolyzing enzyme…