Advanced Journal of Microbiology Research

Abstract: In this investigation we tried to transfer plasmid encoded silver and antibiotic resistance genes from Acinetobacter baumannii BL54 to E. coli k12 J53.2 and Pseudomonas (a soil microflora) by conjugation and transformation in different soil microcosms and study the survival of the isolated bacterium in each soil. Clay loam, fine clay, sandy and clay soils were collected from different area of mahrashtra in India. Microcosm was developed for each type of soil in the glass tube (150 x 25 mm) with 2 g soil moistened w…

Abstract: Enterobacteriaceae were isolated from gills, skin, muscles and the intestine of 83 out of 150 (55%) randomly collected fishes, the most dominants isolates were E. coli, Citrobacter spp, Enteriobacter spp and Klebsiella spp. This together with the highly pathogenic Enterobacteriaceae including Salmonella spp and Shigella spp. Proteus spp, and Alklegens spp. Potential pathogenic organisms were also among the isolates. On the other hand Pseudomonas spp was isolated from 62% of randomly collected fishes. The number and…

Abstract: The aim of this study was to investigate the antimicrobial activity of honey sample from Basrah region against certain microbial isolate. Different concentrations (25.0, 50.0, 75.0 and 100.0%) of honey sample where checked for their antimicrobial activities, using some medically important micro-organisms including Escherichia coli, Pseudomonas spp. and Staphylococcus aureus. The minimum inhibitory concentrations (MIC) of the honey sample were determined on the selected micro-organisms by using broth dilution techni…

Abstract: A promoter-protein fusion library was constructed and analyzed previously which covered about 8% promoter-proximal genes from Escherichia coli in respect to their reading frames, protein productivity as well as expressional regulations under normal and various environmental stress conditions (Talukder et al., 1994, Bioscience Biochemistry and Biotechnology, 58: 117-120). In this study, we further analyzed 13 significantly responsive genes. -galactosidase expression levels were varied among the examined clones, indi…

Abstract: Campylobacteriosis is the most frequently occurring food and waterborne gastroenteritis in Norway and 90% of cases are caused by Campylobacter jejuni. Campylobacteriosis is epidemiologically associated with use of non-disinfected water and consumption of contaminated poultry. In order to provide molecular epidemiological support for this association isolates from local poultry and water were compared with human isolates from domestic and import infections using ribotyping. Automatic riboprints were performed with t…

Abstract: The present study was based on the investigations to determine whether the bactericidal effect of microwave radiations on bacteria was either thermal or due to microwaves. It was also investigated which bacterial sp is more sensitive to microwave. Five bacterial species, Proteus vulgaris PP25D, Staphylococcus aureus bgh010, Bacillu subtilis ase98, Escherchia coli TRE04 and Corynebacterium spN33 were exposed to microwave radiation. These cultures were selected as test organisms due to their importance in food indust…

Abstract: This study is related to a process for the separation of Hibiscus sabdariffa L. fibers using living microorganisms. The selected fungal strains, Datronia sp. and Oligoporous sp., were isolated from natural sources in Thailand and were previously shown to be capable of degrading lignin in pulp and paper industrial wastewater. The process described here is comprising the fungal-treated fibrous of H. sabdariffa L. barks in certain conditions. The fungal inoculum size, retting time and retting temperature were importan…

Abstract: Ninety three cultures of plant growth promoting rhizobacteria (PGPR) isolated from rhizosphere of Pisum sativum, among them one isolate identified as Pseudomonas putida was found to be potential amidase producer. The organism exhibited a battery of PGPR traits including enhanced production of plant growth hormone indoleacetic acid (IAA) and siderophore. P. putida MTCC 6809 exhibited both intracellular and extra- cellular amidase activity. The organism produced maximum extracellular amidase enzyme at 30°C and pH 7.5…

Abstract: The prevalence of diarrheagenic Escherichia coli both in an ambulatory patients passing out loose stools with or without blood and/or mucus in Anua General Hospital, University of Uyo Teaching Hospital and University of Uyo Health Centre from June to September, 2008 were determined using standard microbiological techniques. Susceptibility to seven different conventional and commonly available chemotherapeutic drugs/antibiotics: ampicillin, chloramphenicol, ciprofloxacin, gentamycin, tetracycline, cephalothin and of…

Abstract: Comparative efficacy of 3 serodiagnostic tests (RBPT, STAT and Dot ELISA) in detecting anti - Brucella antibodies in sera was evaluated on a total of 28 serum samples which included 18 samples from brucellosis suspected and 10 from normal healthy (brucellosis unaffected) cattle. Out of 18 sera from suspected cases, only 1 (5.55%) sample was found positive by STAT and 9 (50%) samples were positive by RBPT, whereas Dot ELISA could detect antibodies in all the 18 (100%) samples. Interestingly, RBPT could detect antibo…

Abstract: In developing countries skin diseases can affect more than 60% of the general population and usually are not well managed. Skin diseases are common in children. Epidemiologic studies of the general population, however, are still limited and missing for the region of Kosova. The aim of this study was to investigate disease prevalence in children under 6 years old of out- and in-patients at the department of Dermatological clinic - a tertiary health care center, a cross-sectional study was carried out during a period…

Abstract: Calcitonin gene-related peptide (CGRP) and substance P (SP) which is potent vasodilator neuropeptides play a counter-regulatory role in several models of experimental hypertension. Langendorff-perfused mouse hearts (n = 328) were used to compare coronary flow rates among wild type (WT), a-CGRP gene knockout (KO) and NK1-KO mice under various pressure loading conditions (20, 30, 40 and 50 mmHg). The aorta of each heart was cannulated and all hearts were perfused with PBS at 37°C. Coronary flow rate was measured by p…

Abstract: A five years retrospective study (2003 - 2007) of the prevalence of fowl typhoid (FT) and other poultry diseases diagnosed at the avian unit of the Veterinary Teaching Hospital (VTH), Ahmadu Bello University, Zaria-Kaduna State, Nigeria was conducted. A prevalence rate of 18.4% (129 cases) was recorded for FT out of 700 cases of poultry disease. The highest number of outbreaks of FT was recorded during the rainy season (July - September). 48 cases (29.1%) of FT were recorded in birds 15 weeks and above, 124 cases (…

Abstract: Despite expansive studies over the past century, the epidemiology of invasive meningococcal disease (IMD) has remained elusive in some of its aspects. The following review attempts to summarize the past and current trends in the etiology of IMD. Data was collected through the analysis of peer-reviewed studies and surveillance data on national, sub-national and regional levels performed using various search engines such as pubmed (www.ncbi.nlm.nih.gov/pubmed/), regional WHO homepages (www.who. int) and department of…

Abstract: Carbapenams are one of the ß-lactamase antibiotic resistances to penicillin that were introduced in 1980 to the medical world. Imipenem- a semi-synthetic derivative of thienamycin- is one of the most important broad spectrum ß-lactamase in carbapenems. The irregular usage of drugs, especially in these types of gram-negative bacteria will lead to a serious problem in the treatment of pathogenic bacteria. Comparison of in vitro activity of imipenem productions is the aim of this study. 135 strains of various gram pos…