Advanced Journal of Microbiology Research

Abstract: The amylase family of enzymes is a great significance due to its wide area of potential application. α-amylase from PENICILLIUM CAMEMBERTI PL21 obtained from I. N. A. (The Technological Laboratory of Agriculture National Institute Paris- France) and using orange waste as substrates was produced under optimum conditions, after 168 h of incubation and subjected for purification and characterization. The enzyme was purified by ammonium sulfate precipitation, dialysis, sephadex G-100 and DEAE-Sepharose CL-6B column chr…

Abstract: The objectives of this study were to examine methicillin-resistant Staphylococcus aureus (MRSA) strains recovered from major hospitals in King Saudi Arabia (KSA) to determine the percent of community acquired MRSA (CA-MRSA) phenotypically by conventional methods and genotypically by multiplex polymerase chain reaction (multiplex-PCR) for direct and simultaneous detection of S. aureus 16S rRNA, Panton–valentine leucocidin (PVL ) and staphylococcal cassette chromosome mec (SCCmec) type IVa genes. Therefore, 135 strai…

Abstract: Molecular dynamics simulations may be used to probe the interactions of membrane proteins with lipids and with detergents at atomic resolution. Examples of such simulations for ion channels and for bacterial outer membrane have already been studied. The molecular potassium channel function is universally conserved. Potassium channels allow potassium flux and are essential for the generation of electric current across excitable membranes. Potassium channels are also the targets of various intracellular control mecha…

Abstract: 16S rRNA methylase-mediated high-level resistance to aminoglycosides has been reported recently in clinical isolates of Gram -negative bacilli from several countries. Five (2.5%, 5/198) of 198 isolates of Proteus mirabilis from a teaching hospital in Wenzhou, China, were positive for 16S rRNA methylase genes (one for armA, four for rmtB) and highly resistant to gentamicin, amikacin and tobramycin (MICs, 256 g/ml). One of five isolates harboring 16S rRNA methyalse genes were extended-spectrum - lactamases (ESBL) pro…

Abstract: Mycobacterium paratuberculosis (MAP) is a gram positive, acid-fast bacterium and cause of Johne’s disease in some animals. The important signs of this disease in bovine are diarrhea, weight loss, bowel inflammation, fever and reduce of milk production. The symptoms of this disease are very similar to Crohn©s disease in humans. The aim of this study was to use nested -PCR as an accurate and fast method to trace MAP in bull semen. Semen samples from 112 bulls were collected and DNA was extracted. Then, nested-PCR was…

Abstract: Tamarindus indica L. (Tamarind), an underutilised fruit tree which belongs to the Leguminosae family, grows wild in the savannah region of Nigeria. T. indica pulp was obtained from 19 towns of the 20 savannah states of Nigeria. The methanol and hexane crude extracts obtained from it pulps were evaluated in vitro to determine their inhibition activities on human pathogenic microorganisms made up of five bacteria and three fungi. All the bacterial strains were sensitive to both extracts at concentrations ranging from…

Abstract: A tasty peptide, beefy meaty peptide (BMP), which was initially isolated from beef digested by papain, has potential as a new flavor enhancer. BMP is a small peptide consisting of eight amino acids. In this study, DNA encoding BMP was synthesized and cloned into the expression vector pPICZ A to obtain the recombinant expression vector pPICZ A-BMP. The recombinant vector was linearized and then integrated into the genome of Pichia pastoris strain X-33 by electroporation. The recombinant strain P.p2 was then incubate…

Abstract: The bacterium Listeria monocytogenes is a Gram-positive, intracellular, ubiquitous, and facultative food-borne pathogen of humans and animals. They may occur naturally in environmental sources such as soils, faeces and intestinal tracts of animals and humans. The pathogen causes listeriosis characterized by gastroenteritis, meningitis, abortion, and sometimes death in systemic cases. Neonates, infants, immunocompromised individuals, pregnant women, and the elderly in particular are most susceptible to listeria infe…

Abstract: Microorganisms isolated from sawdust have been demonstrated to be effective in its degradation. Eight bacteria and eight fungi were isolated from wet decaying sawdust. These were tested for their capability to degrade sawdust. Among the bacteria, C ellulomonas sp. was found to be the most effective degrading agent based on its high percentage degradation (18.3%). This was followed by Micrococcus sp. (16.0%) and Pseudomonas sp. (14.6%), Cytophaga sp. and Bacillus sp. had the lowest percentage degradation of 0.2 and…

Abstract: The decolourisation efficiency of the wild fungal strain Mucor mucedo was investigated by the treatment with physical mutagen ultraviolet radiation (UV) and chemical mutagens [ethyl methyl sulfonate (EMS), diethyl sulfonate (DES) and colchicines]. The mutants that were exposed to 12 and 15 min UV radiation showed reduction in extension and branching of hyphae. EMS at concentrations of 100 and 150 mg inhibited the germination of spores in M. mucedo. Surprisingly, at concentrations of 100 and 150 mg DES, spores were…

Abstract: Antimicrobial activity of organic (methanol and chloroform) and aqueous stem back extracts of Erytrina senegalensis against some pathogenic microorganisms was investigated using the filter paper disc diffusion method. Phytochemical studies revealed the presence of saponins, tannins, glycosides, phenols and alkaloids. The extracts demonstrated antimicrobial activity against both bacteria (Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa) and fungi (Aspergillus…

Abstract: Biosurfactants as surface active molecules that are synthesized by microorganisms. These substances include many advantages in comparison with chemical surfactants. For instance they have lower toxicity, higher biodegradability, better environmental compatibility, higher foaming activity, and specific activity at extreme temperatures, pH ranges, and the ability to be synthesized from renewable feed stocksin. In this study, the production of biosurfactant, produced by PTCC 1561 was studied. This bactrium was grown i…

Abstract: Oropharyngeal candidiasis (OPC) is the most common HIV related oral lesion. Most patients are infected with a strain originally present as a commensal of the oral cavity. The resistance of Candida isolates to antifungal drugs is important due to morbidity and mortality. The aim of our study was to investigate the antifungal susceptibility profiles of oral Candida spp. which were isolated from HIV-infected patients. In vitro susceptibility tests were performed using the broth microdilution method recommended by the…

Abstract: Fungus, named TZ1, was isolated from coal mine soil at the Fushunxi colliery, Liaoning Province, North of China. The lignite’s bioconversion rate was about 23.3% by TZ1. The morphological, biochemical, physiological characterization and analysis based on 18S rDNA gene sequence indicated that the strain TZ1 was most closely related to Hypocrea lixii (100% similarity in gene sequence), bioconversion lignite experiments with this new strain showed that it could play an important role in degradation of Chinese lignite…

Abstract: Soil samples from organic saline paddy fields from 14 provinces of the northeast region of Thailand were collected and used for isolating photosynthetic bacteria (PB) prepared as a Soil and Straw Products (SSPs). PB from these SSPs were further grown in double strength G5 broth under microaerobic-light conditions before purification. A total of 130 isolates were then screened for growth in G5 broth under microaerobic-dark conditions for 24 h and 15 isolates were further selected by secondary screening in G5 broth u…