Advanced Journal of Microbiology Research

Abstract: Respiratory disease is an emerging problem in camels, although information of the normal bacterial flora and bacterial agents implicated in pneumonia is rare in Nigeria. Five hundred lung samples were collected at random from two randomly selected camel slaughter house in Nigeria. Swabs samples were cultured and identified. Arcanobacterium pyogenes, Mannheimia haemolytica and Pasteurella multocida were recovered from pulmonary lesions, however Staphylococcus aureus and other Staphylococcus spp. were the most common…

Abstract: Enterococci are among prominent causes of nosocomial wound infections. Since the rapid detection of causative agents could make earlier administration of choice antibiotics and quick recovery of patients, so the application of rapid diagnostic methods is important. Therefore, this study was designed to evaluate fluorescent in situ hybridization (FISH) for the detection of Enterococcus in wound swab samples. The time needed for FISH procedure is about 3 h. Specimens taken from 33 hospitalized patients were examined…

Abstract: We are unable to detect all microorganisms in media. In consequence, molecular methods like PCR based techniques can mend our difficulties in this era. Herein, we describe simultaneous detection of major food-borne pathogens Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7. Iranian food materials used for comparison of traditional microbiological methods (such as culture and serology) and multiplex PCR method in the detection of pathogens were prepared from several local restaurant, including eg…

Abstract: This study is the first to report on the resistant phenotypes and genotypes of Enterococcus faecium clinical isolates in Shantou, China. A total of 39 E. faecium clinical isolates were collected from January 2004 to January 2006 and analyzed for their resistance to 10 antibiotics and for genes coding for resistance to the associated antibiotics. E. faecium isolates were resistant to 5 or more antibiotics, and most showed high minimal inhibitory concentrations to many antibiotics as well. Resistance to erythromycin…

Abstract: Fusidic acid -resistant Staphylococcus aureus is poorly documented in community acquired infections in Morocco. From 1st January, 2007 - 31 October, 2008, we collected 140 S. aureus isolates at Institute Pasteur, Casablanca, 18 of them exhibited resistance to fusidic acid and were negative for gene encoding methicillin resistance (mecA). seh toxin gene was found in 14 strains (14/18) along with an accessory gene regulator (agr) group III, 9 of them were found with two other toxin genes (sek plus seq). In conclusion…

Abstract: Because antimicrobial resistance patterns are continually evolving and multi-drug resistant (MDR) organisms undergo progressive antimicrobial resistance, continuously updated data on antimicrobial susceptibility profiles will continue to be essential to ensure the provision of safe and effective empiric therapies. This current study reports on the assessment of antibiotics susceptibility profiles of some selected clinical isolates from laboratories in Nigeria. Thirteen antibiotics were bought from different pharmac…

Abstract: In the growing concern for microbial food safety of traditional foods, the present study has attempted to characterize toxigenic profile of native food isolates of Bacillus cereus. In a total of 65 traditional foods, 26 isolates were characterized by morphological, cultural and biochemical attributes as B. cereus. Of these, 12 isolates (46%) were confirmed as B. cereus by PCR with 16S rDNA and phosphatidylinositol phospholipase C primers in PCR. Among B. cereus isolates, 8 (67%) and 6 (50%) were positive for bindin…

Abstract: The aim of this study was to investigate the significance of multiple-mutations in the katG gene, predominant nucleotide changes and its correlation with high level of resistance to isoniazid in Mycobacterium tuberculosis isolates that were randomly collected from sputa of 42 patients with primary and secondary active pulmonary tuberculosis from different geographic regions of Iran. Drug susceptibility testing was determined using the CDC standard conventional proportional method. DNA extraction, katG gene amplific…

Abstract: Presence of Vancomycin Resistant Enterococcus (VRE) is increasingly being reported. In the present study we reported the prevalence of Vancomycin Resistant Enterococcus causing Surgical Site Infections (SSI) in a tertiary care hospital. Pus from surgical site wound was cultured and Enterococcus species was identified as standard microbiological methods. The antimicrobial susceptibility for Vancomycin was determined by Minimum Inhibitory Concentration (MIC) by broth dilution method. Patients (n = 2566) who had under…

Abstract: The use of oral contraceptive pills are increasingly becoming popular among women in both urban and rural Nigerian settings, and its perceived association with gynaecologic infections not withstanding. This study was therefore carried out to ascertain the rate of urogenital candidiasis among women on oral contraceptive pills (OCP) in Gboko town. All the willing women on OCP attending family planning clinic and Comprehensive health centre in Gboko were consecutively recruited in the month of September, 2009. Questio…

Abstract: The present study was done for characterization and antibiotic sensitivity pattern of the Salmonella spp. found as contaminants in chicken carcass. The overall incidence of Salmonella contamination of poultry carcass was found to be 4.90% with the higher percentage of Salmonella being isolated from chicken meat (8.00%) followed by liver and spleen (6.25%). The isolates were identified as Salmonella Typhimurium, Salmonella Paratyphi B and Salmonella (Rough). Eight Salmonella isolates obtained from poultry were confi…

Abstract: Spoilage of food due to contamination by bacteria present in the environment is a major problem which affects human health and efforts to identify antibacterial components from natural sources has gained momentum. In this regard we isolated a new strain of BACILLUS LICHENIFORMIS IITRHR2 (accession number FJ447354) which produced bacteriocin like inhibitory substance (BLIS). Partially purified BLIS showed a major peak on HPLC with antimicrobial activity. This proteinaceous substance (~1.2 kDa) was thermo-stable and…

Abstract: This research paper investigated the physico -chemical and microbiological properties of 10 samples of honey obtained from Ibadan, Oyo State. The physicochemical properties evaluated were colour, water activity, pH, moisture content, ash content and electrical conductivity, while the standard plate count (SPC), total coliforms, Bacillus spp., yeast and mould fungi were the microbiological properties evaluated. Results showed a range of honey colours from light amber to dark amber. There was significant difference i…

Abstract: It is well known that putative pathogenic bacteria are ubiquitous and widely distributed in the hospital environment. This study aimed to detect bacterial persistence in the nosocomial environment (different critical areas of the hospital) after mopping by the cleaning staff. Susceptibility patterns to antimicrobial drugs and disinfectants commonly used in health services were also investigated by disk diffusion and agar dilution tests. Rinse water from mops was processed for isolation of Enterobacteriaceae (GNR)…

Abstract: Shiga toxin -producing Escherichia coli (STEC) is recognized wordwide as human pathogen. During the processing of carcasses, fecal contamination or transfer of bacteria from the animals’ hide to carcasses can promote transmission of pathogenic E. coli to food supplies. A survey was performed to determine the sensibility profile to several antimicrobial drugs of STEC in carcasses obtained from an abattoir in Brazil between March 2008 and August at 2009. A total of 120 STEC were isolated. All isolates were confirmed…