Advanced Journal of Microbiology Research

Abstract: The aim of the present study is to determine the activities of three kinds of antibiotics against Staphylococcal biofilm and planktonic cultures in vitro, and to indicate the enhancement of biofilm formation in response to stress factors such as glucose and sub-inhibitory concentrations of antibiotics by using scanning electron microscope. Biofilm forming staphylococci were identified by using the modified microtiter plate method. And the effect of different concentrations of several antibiotics (including ciproflo…

Abstract: The aim of this study was to examine the reduction of enterotoxigenic Escherichia coli (ETEC) colonization by oral administration of Lactobacillus casei as probiotic in a murine model. In this study, thirty-six BALB/c mice were divided to two test groups and one control group. One of the test groups was fed probiotic bacteria at six days and other groups at three days; whereas, the control group did not receive any probiotic. 72 h after the last oral administration of probiotic, the all three groups were fed by the…

Abstract: A hyper-chitinase producing isolate Paenibacillus sp. D1 was obtained from common effluent treatment plant of seafood industries at Veraval (Gujarat, India). The isolate exhibited chitinase production over a wide temperature (25 - 45°C) and pH (6 - 9) range with maxima at 30°C in medium with initial pH 7.0. The crude chitinase had activity in broad pH (4 - 10) and temperature (30 - 60°C) range with optima at pH 5.0 and 50°C, respectively. The enzyme was highly thermostable with t1/2 of 36 to 60 h at 40 and 45°C. Cr…

Abstract: A central composite design using two variables (concentrations of isolate P1 and Fermented Pineapple Extract (FPE) each at three levels was used to study their effects on the treatment efficiency of latex rubber sheet wastewater under microaerobic-light conditions. The optimum combination over a 72 h period consisted of 3% P1 and 0.13% FPE and resulted in the removal of 80% Chemical Oxygen Demand (COD), 82% Suspended Solids (SS) and 85% Un-ionized Hydrogen Sulfide: H2S in wastewater (UHS). The selected experimental…

Abstract: This study has carried out to evaluate the antibacterial effect and the interaction between Tinospora crispa and Swietenia mahagoni extracts against resistant strains of Methicillin-resistant Staphylococcus aureus (MRSA). The minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were determined in the setting of clinical MRSA isolates. Separated and mixture forms of T. crispa and S. mahagoni extracts were assayed to determine their antibacterial activity against eight clinical MRSA…

Abstract: Newcastle disease virus (NDV) causes major losses in the poultry industry and is regarded as endemic in many parts of Africa. Differences in virulence of the virus occur and during any disease outbreak, determining virulence is essential for effectively controlling the disease. The virulence of the virus is dependent on cleavage of the fusion site and is characterised by different sequences in the genome. Thus a reliable and rapid method to determine virulence is to sequence the fusion site. The alternative method…

Abstract: Bifidobacterium species are known for their beneficial effects on health and their wide use as probiotics. Molecular methods applied to accurately and rapidly identify these micro-organisms are necessary. This study was conducted in order to isolate Bifidobacterium from newborn babies’ microbiota and also to identify the new isolated strains by PCR and ARDRA. The bile and the low pH tolerance for the new isolates were evaluated as evidence of possible probiotic potencial. A total of 30 strains were isolated from fo…

Abstract: Q fever is a zoonotic disease caused by the rickettsial organism Coxiella burnetii, the objective of this study was to determine the prevalence rate of C. burnetii in bulk milk samples from dairy goat herds in Fars, Ghom, Kerman, Khuzestan and Yazd provinces, Iran. In the present study, 296 bulk milk samples from 89 dairy goat herds were tested for C. burnetii using a nested PCR assay. The animals which their milk samples collected for this study were clinically healthy. In total, 6 of 296 (2.0%) goat milk samples…

Abstract: The aims of this study were to study the process of microbial contamination of healthcare workers’ (HCWs) hands during routine patient care and simultaneously to evaluate the state of antibiotic susceptibility of microorganisms isolated from the hands of HCWs. Samples were collected from the hands of 154 HCWs working at Gaziantep University Hospital. A standard bag broth technique was employed. In addition to conventional methods, VITEK 2 automated system and API ID 32C kits were used for identification of the isol…

Abstract: This research was conducted to study the fruiting of an edible truffle TERFEZIA BOUDIERI Chatin in fields using mycorrhizal plants of HELIANTHEMUM SESSILIFLORUM Desf. Pers. in two soil types (gypsy and sandy loam soils). Two cultivation methods have been tested. The first is the transplantation of inoculated plants (with T. BOUDIERI ascospores) maintained in greenhouse and the second is the seedling of inoculated H. SESSILIFLORUM seeds in experimental field (24 m2). The first ascocarps was detected one year after (…

Abstract: Ten herbs which are widely used in Ayurvedic system of medicine and are collectively known as “Dashapushpam “in Kerala, (India), were screened for their antimicrobial properties against nine spp. of pathogenic fungi and seven spp. of pathogenic bacteria. In the preparation of extracts, the entire shoot systems were used for Cardiospermum halicacabum and Evolvulus alsinoides and only leaves for others. Crude plant extracts were prepared by cold extraction with acetone. Two sets of pathogenic fungi-Set-1 and Set- 2-w…

Abstract: It is crucial to have a knowledge of pandemia. The aim of this study was to assess knowledge, attitude and behavioral responses of health care workers (HCWs) toward pandemic H1N1 influenza (PI). A cross-sectional study was performed in September 2009 in Diyarbakir, Turkey. All HCWs replied to a self-administered standardized, structured questionnaire. A total of 783 participated, with 31.55% having low knowledge and 22.98% having high level about PI. It was observed that HCWs sex was affecting knowledge level score…

Abstract: Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, hemolytic-ureamic syndrom and thrombotic thrombocytopenic purpura. In this study the effect of microwave irradiation of beef samples which were inoculated with E. coli O157:H7 were investigated. The portions of fresh beef slices weighting 200 g each and about 10 × 10 × 2 cm in size, were soaked in fully growth of E. coli O157:H7, in BHI broth. The swab samples were taken from the contaminated samples, after different times of radi…

Abstract: Ghanaian currency notes are handled by all manner of people including ready-to- eat food sellers who serve food and handle the currency notes as they sell making the notes dirty and cross-contaminated. Hence this study aims at determining bacterial species and level of contamination of the notes in circulation. Ghanaian currency notes were collected at random from ready- to-eat food sellers in Kumasi. Buffered peptone water (BPW) washings of the notes were inoculated onto plate count agar (PCA) for total viable cou…

Abstract: Our study focused on assessing frequency of Klebsiella oxytoca, to study incidence of K. oxytoca producing ESBLs, to investigate frequency of blaTEM,blaSHV and blaCTX-M and to determine resistance of K. oxytoca producing ESBLs toward non-beta-lactam antibiotics, in different seasons in Ilam hospital. Twelve clinical isolates of K. oxytoca were found during March 2007 to April 2008 in Ilam hospital in Ilam city in west of Iran. The strains were isolated from admitted patients in surgery wards, lesion and respiratory…