Advanced Journal of Microbiology Research

Abstract: Soft rot occurred severely in potato tubers stored in traditional and ameliored storehouses at Sikasso, Mali. 17 infective bacterial isolates were isolated from potato rot tissues (Solanum tuberosum L var. Odessa). Out of all, the isolate Od23 was found pathogenic and was characterized as rod-shaped, Gram positive, endo-spore formers and yellow pigment producers. This isolate which was found to be the principal organism responsible for potato rot in storage in Sikasso, grew at a temperature range from 5 to 45°C, wi…

Abstract: Ectomycorrhizal mushroom Cantharellus tropicalis was grown in axenic culture to study the effect of pH, temperature, nitrogen, carbon, phosphorus and heavy metals/trace elements on production of acid phosphatase and mycelial growth. The results of present study showed optimum mycelial growth with pH 4 at 15 and 35 ± 2°C. The ectomycorrhizal mushroom mycelia utilized lactose and yeast extract as best carbon and nitrogen source for biomass production. Ferrous sulphate supported the maximum mycelial growth when differ…

Abstract: Most of the Iranian almond cultivars are self-incompatible followed by pollination, fertilization, fruit set and lower yield problems. Therefore, selecting suitable cross-compatible cultivars for orchard establishment is necessary especially by new cultivars/genotypes obtained from breeding programs. In this study fruit set and pollen tube growth of ten late- bloom almond genotypes, obtained from a breeding program (D, E, F, I, G, L, K, O, P and Q) were investigated under filed and lab controlled pollination condit…

Abstract: Two processes of enclosure of probiotic Lactobacillus plantarum CMU-FP002, probiotic granules and calcium alginate beads, were studied. Sodium alginate solution at 1.0, 1.5 and 2.0 % (w/v) was used as a binder. The results showed that 20 log cfu/ml initial concentration of cells could be entrapped by the granules and beads with 12 to 13 log cfu/g and 16 cfu/g, respectively. The physical properties of granules and beads revealed that the strength increased when sodium alginate concentration was increased. On the oth…

Abstract: The aim of this study was to compare allergic rhinitis clinics caused by two most common allergens: house dust mites (HDM) and pollens. Three hundred and fifty patients were evaluated. These patients were pure pollen or HDM allergic according to skin prick test (51 HDM+, 299 Pollen+). Mainly, HDM were allergens for perennial allergic rhinitis, while pollens were for seasonal allergic rhinitis (p < 0.01). Both groups were found with similar symptom frequency except palatal, ocular, throat itching and eye redness ind…

Abstract: A cluster model for active site of oxidized azurin was presented and investigated the geometric structure and thermochemical parameters. Quantum-mechanical calculations were performed at the HF and B3lYP/6-31G levels of theory in the gas phase and eight solvents at four temperatures. Also, nuclear shielding parameters of the active site of oxidized azurin have been taken into account using GIAO and CSGT methods at the HF and B3LYP/6-31G levels of theory in the gas phase and in different solvents such as water, DMSO…

Abstract: A total of 300 freshly voided sheep faeces were collected and screened for the presence of Campylobacter spp. using standard microbiological techniques. The samples were obtained randomly from 3 farm settlements in the Venda Region, South Africa in 2008 and 2009. The recovery rate was 30.0% (90 of 300) for all faeces. Of these, 65 (72.2%) were from diarrheic and 25 (27.8%) were from non-diarrheic faeces. Out of the 90 Campylobacter spp. isolated, 41(45.6%) were Campylobacter jejuni and 49 (54.4%) were C. coli. Sixt…

Abstract: A serological and bacteriological study was performed in Guilan industrial slaughter house, in Rasht, North of Iran in 2009. To investigate the seroprevalence of leptospirosis in slaughtered cattle in Guilan, 59 and 39 random serum samples were collected from cows and bulls, respectively. None of the cattles was vaccinated against leptospirosis. Also urine samples were collected from all of the blood-sampled cattle and cultured. All serum samples were serologically tested by microscopic agglutination test (MAT), as…

Abstract: Colour removal of industrial effluent has been a major concern in waste water treatment, especially for the waste water that originates from textile and dye stuff plant with a continuous discharge of great quantity of remaining dyes to the environment. The efficient treatment of the effluent is an eco- friendly method for the treatment of textile effluent. Bacillus species was isolated from the processed food-pickles and were characterized by means of biochemical reactions. Spore formers, non spore formers and thei…

Abstract: The aim of this research was to detect oral Streprtococci bacteriophages from Persian Gulf. Dental plaque samples were collected using sterile explorer and cultured in brain heart infusion (BHI) Broth. The oral Streptococci were isolated in culture media. The Persian Gulf water sample was gathered using a sterile bottle from the depth of 50 cm under the inframarine surface at Boushehr Port, Boushehr state, Iran. The Persian Gulf water was centrifuged and its supernatant was filtered through a 0.45 micrometers membr…

Abstract: The antimicrobial activity of water, ethanol and chloroform extracts of Acalypha indica was tested against four bacterial and fungal strains using the disc diffusion method. The antibacterial activity against gram positive bacteria was more pronounced (p < 0.05) in water and ethanol extracts. Antifungal activity was more significant (p < 0.05) only in chloroform extract. This antimicrobial activity was compared to standard antibiotics (penicillin, enrofloxacin, ampicillin and chlorampenicol) and antifungal drugs (k…

Abstract: Bacillus species suppress phytopathogens by producing lipopeptide antibiotics, hydrolytic enzymes, siderophores and other secondary metabolites. Three bacterial strains Bacillus subtilis NH-100 (EU627167), B. subtilis NH-160 (EU627169) and Bacillus sp. NH-217 (EU627170) with proven ability to suppress red rot disease on sugarcane plants were further characterized to elucidate the multiple modes of action involved in their biocontrol activity. Plate assays pointed out the production of protease and antibiotics. Lipo…

Abstract: Tetracycline-resistant (TetR) Helicobacter pylori isolates have emerged in many parts of the world. We have previously demonstrated that among the mechanisms involved in the resistance of H. Pylori to Tet, its active efflux may be an important mechanism. This work, aimed to determine whether presence and/or expression of hp1165 are associated with efflux-mediated resistance to Tet in clinical strains of H. pylori. Twenty five TetR strains including seven low level-resistant, eight intermediately- resistant, and ten…

Abstract: AdeABC efflux pump in a total of 50 Acinetobacter baumannii strains were investigated and the role in contributing to hydrolysis carbapenems were further analyzed. All strains were divided into 3 groups according to their susceptibilities to Imipenem, Amikacin, Minocycline and Levofloxacin: Group A (22 imipenem-resistant strains), B (13 isolates which were imipenem-sensitive but resistant to at least one of the other three antibiotics) and C (15 isolates, sensitive to all the antimicrobials). Five gene types were o…

Abstract: The aim of the study was to investigate the causative pathogens, profile of antimicrobial susceptibility of them and the extent of tissue lesions in diabetic foot infections. This is a prospective study in which infected diabetic foot presenting with Wagner grade 1 to 5 ulcers were investigated. 78 consecutive diabetic patients who were seen in the orthopaedic clinic were cultured during ordinary visits. Bacteriological diagnosis and antimicrobial susceptibility profiles were carried out and analyzed using standard…