Advanced Journal of Microbiology Research

Abstract: Present study was aimed to determine antibiotic susceptibility and penicillinase production by Neisseria gonorrhoeae strains isolated from gonorrheal patients in Zahedan (south-east Iran). In a descriptive study from 2007 to 2010, 400 suspected patients were studied by history review, medical examination, Gram staining and culture in Thayer-Martin medium. Antibiotic susceptibility test of isolated strains was done by disk diffusion method and penicillinase test in penicillin resistant isolates by aciodometric metho…

Abstract: Mycobacterium tuberculosis employs a number of strategies to subvert host signaling events, leading to its persistence within macrophages. Upon infection, Mycobacterium bovis BCG induce the expression of suppressor of cytokine signaling 3 (socs3), in a Toll-like receptor 2 (TLR2)-Notch1-dependent manner. Purified phosphatidyl inositol di-mannosides (a TLR2 agonist) act as an inducer for the Notch1-socs3 pathway. This prompted us to analyze other TLR2 agonists seeking for additional molecules that may affect this pa…

Abstract: During processing of cattle carcasses, contamination may occurs with the transfer of microbiota of animals feaces to carcasses. This contamination many times may be by Escherichia coli carriers of virulence factor as stx and eae genes being classified as Shiga like toxin. Shiga toxin-producing Escherichia coli (STEC) is recognized wordwide as human pathogen. A survey was performed to determine the sensibility profile to several antimicrobial drugs of STEC in carcasses obtained from an abattoir in Brazil between Mar…

Abstract: Microbial biodegradation is an important and promising decaffeination approach because of its low cost and high security. The efficiency of this approach mainly depends on the characteristics of the strain. Twenty bacterium strains were isolated from soil of tea garden and exhibited high caffeine-tolerance, and these strains could grow on the medium supplemented with 20 g L-1 caffeine as the sole source for nitrogen and carbon. According to the physiological-biochemical characteristics and 16S rRNA gene sequence bl…

Abstract: Troponin (Tn) is an essential protein in calcium ion settings of skeletal and heart muscle contractions. It has three subcategories (TnC, TnI, TnT) which are connected to filament actin by tropomyosin. TnC controls the calcium in muscle contraction and is the Ca2+-binding subunit of the troponin complex. ATP facilitates separation of myosin from actin filament, eventually making thick filament to slip off thin filament. This results in short sarcomere. In this study, it is investigated that thermodynamics propertie…

Abstract: Plant-derived compounds used as alternatives to chemical preservatives have been extensively researched for use as natural medical ingredients or food preservatives. Conditions for the ultrasound-assisted extraction (UAE) of chicory (Cichorium intybus) root (including type of solvent, impregnation time, number of sonication steps and ultrasonic power) were optimized to determine the best extract antibacterial activity by using orthogonal matrix design [L16 (45)]. The combination of 70% ethanol v/v, a 36 h impregnat…

Abstract: The body normally hosts a variety of saprotrophic micro-organisms that may cause infection. Athlete©s foot causes scaling, flaking and itching of the affected skin. Blisters and cracked skin may also occur, leading to exposed raw tissue, pain, swelling and inflammation. Secondary bacterial infection can accompany the fungal infection. This work examined the anti-fungal activity of Ardisia crispa (AC) against common fungi that cause Athlete’s foot and several other pathogenic fungi. The antimicrobial activity of wat…

Abstract: Women living with HIV are faced with lack of adequate information about HIV prevention. This study assessed the level of knowledge of prevention of mother-to-child transmission (PMTCT) in Serowe, Botswana. The objectives of this study were to assess the level of knowledge of the PMTCT programme and vertical transmission among HIV-positive women in Serowe, Botswana and as well as describe the practices of family planning among the participants. The study was a cross-sectional descriptive survey using qualitative met…

Abstract: In Taiwan, a fish conservation farm had about 6,250 Taiwan paradise fishes, Macropodus opercularis (L.), which were separately cultivated in an outdoor tank containing 3,250 fishes, and an indoor tank containing the rest. The water supplying both tanks was the same running water source from the adjacent hills. Following a change in the fish feed (the commercial aquaculture feed became eel’s feed) to all fishes in May 2010, rotten body surfaces were only found in diseased and dead fishes in the outdoor tank. Interes…

Abstract: Enteroviruses are among the most common viruses infecting the human intestine; they are very widespread in nature and resistant to external agents. They are eliminated in the faeces and contaminate water and food. These viruses cause various clinical syndromes and constitute a big public health problem. The aim of our study was to search for enteroviruses in the water samples. For this, a total of 225 L of tap water coming from the national network office for drinking water (ONEP) and 18 samples of waste water orig…

Abstract: Buruli ulcer (BU) is caused by a mycobacterium called Mycobacterium ulcerans . The events of BU are the skin lesions. The lack of early diagnosis and treatment cause severe disability. Today the emergence to BU in Africa and particularly in Côte d’Ivoire needs faster diagnosis to control and to prevent the infection by M. ulcerans. The surveillance of BU is difficult, because the transmission of M. ulcerans occurs in rural regions where the transport of fresh collected sample is long, and the detection with culture…

Abstract: Traditional medicine has a key role in health care worldwide. Obtaining scientific information about the efficacy and safety of the plants is one of the researcher's goals. In this research, the flowering aerial parts of Eremurus persicus were collected from Golpayegan (Isfahan, Iran) in May 2010. The extract was tested for its antibacterial activity against 4 Gram- positive bacteria strains (Staphylococcus aureus, Staphylococcus epidermidis and Bacillus cereus, Streptococcus pyogenes) and 5 Gram-negative bacterial…

Abstract: A newly isolated Aspergillus awamori MTCC 9997 strain from spoiled cooked rice was cultured under solid state using cassava peel powder to produce α-amylase. The effect of extraction medium, solid to solvent ratio, extraction time, temperature and physical state on α-amylase recovery was examined. Among various organic, inorganic solvents and buffer solutions tested, maximum extraction of α-amylase was achieved when phosphate buffer of pH 7.0 was added. A solid to solvent ratio (cassava peel : phosphate buffer) of…

Abstract: This study is aimed at evaluating the immunogenicity of two inactivated (mono- and polyvalent) vaccines containing bovine virus diarrhea virus (BVDV) reference and field strains. Three experimental groups were formed: 10 calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated polyvalent vaccine per animal (Group 1); 10 calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated monovalent vaccine per animal (Group 2) and 9 unvaccinated calves (Control, Group C…

Abstract: To determine the relations between Haemophilus influenzae (Hi) genotypes (antibiotic resistance and active IgA1 protease) and infectious diseases, strains from infected blood, pus, sputum, bronchial washing and thorax patient samples with invasive diseases were cloned, and assayed for IgA1 protease activity and the enzymic subtype, as well as antibiotic resistance. Clinic samples of patients aged 1 to over 71 with invasive diseases of pneumonia, sinusitis, bacteremia, bronchitis, chronic obstructive of pulmonary di…