Advanced Journal of Microbiology Research

Abstract: Hospital infection is one of the important agent morbidity and mortality in patient. Bacillus cereus is one of the bacteria that cause nosocomial infection. Hospital surfaces and staff hands have important to creation nosocomial infections. Hospital surfaces have potentional capacity to carry bacteria and staff hands are the most reason for the transmission of bacteria to hospital. The present study was performed at one tertiary care hospitals in Isfahan, Iran. During a 30 month period. According to statistical for…

Abstract: In the present study, one recombinant baculovirus BacSC-GP5, expressing His6-tagged GP5 with the transmembrane domain (TM) and cytoplasmic domain (CTD) derived from baculovirus envelope protein gp64, was constructed and its immunogenicity and protective efficiency was evaluated in piglets. The results obtained show that, His6-tagged recombinant GP5 was expressed and anchored on the plasma membrane of Sf-9 cells, as revealed by Western blot and confocal microscopy. Immunogold electron microscopy demonstrated that, t…

Abstract: Streptococcus thermophilus is used primarily as starter cultures to counter the harmful bacteria grown in cheese and yogurt making/preservation processes. These bacteria produce some exogenous toxins called bacteriocins having the antimicrobial activities against both Gram positive and Gram negative bacteria. In our study S. thermophilus growth was obtained at pH 5.5 and temperature 40°C. Bacteriocin activities were checked after their treatment with different enzymes, organic solvents, sodium chloride (NaCl) and d…

Abstract: Varied mixtures of different concentrations 1, 1.5 and 2% of acetic (AA), lactic (LA), propionic (PA) and formic (FA) acids at 1:1 ratio were spray- washed on inoculated meat to evaluate their efficacy in reducing loads of Escherichia coli O157:H7 and Staphylococcus aureus on meat tissue. It was found that increasing the concentration of the used organic acids increased the bacterial lethality proportionally. And significant difference (P[...]

Abstract: An isoprenoid named undecaprenyl phosphate (Und-p) is the only known lipid carrier in bacteria. It is involved in the translocation of hydrophilic intermediates of cell wall components across the hydrophobic phospholipid bilayer of the cell membrane for subsequent polymerization in cell wall synthesis. Und-p is made available by both de novo synthesis and recycling. Evidences are emerging that in addition to dephosphorylation of undecaprenyl pyrophosphate (Und-pp), the phosphorylation of undecaprenol (Und-OH) into…

Abstract: Ninety four clinical isolates of Pseudomonas aeruginosa cultured from patients admitted to the Intensive Care Unit (ICU) (N= 37) and Otolaryngology (ORL) (N= 57) during one year (2001-2002) at the Rabta hospital (Tunisia) have been investigated by using serotyping, pyocintyping, drug susceptibility, M13-PCR and PFGE typing. Result shown that most of the isolates at the ICU belonged to serotype O:12 (11/37) that showed high resistance to commonly used antimicrobials ( -lactamins, aminosids, and quinolone) and a pred…

Abstract: The effect of Avid® on the induction of synnema-like structure of Aspergillus flavus (61 strains) grown on various types of agar media was investigated. Other related species of Aspergillus within the section Flavi, A. parasiticus (14 strains), A. nomius (14 strains), A. psuedotamarii (2 strains), A. tamarii (20 strains) and A. oryzae (5 strains), were also evaluated for comparison. When Avid® was added into five different solid media (Czapek, V8, Aspergillus flavus and A. parasiticus, corn meal and potato dextrose…

Abstract: The quality of potable water and treatment of waterborne diseases are critical public health issues. Bacterial contamination of drinking water sources is the most common health risk. The research determines bacteriological quality of drinking water sources in Serbo town, south west Ethiopia. A Cross-sectional study design on bacteriological analysis of drinking water was conducted in Serbo town from September to October, 2010. 100 ml of water specimen was collected from each water sources and transported for testin…

Abstract: The goal of this study was to determine the effect of bispyribac-sodium on the nitrogenase activities and growth of cyanobacteria isolated from paddy fields. Ten cyanobacterial species were used in this study. Cyanobacterial species were isolated from soil and water samples obtained from rice fields in Corum, Turkey. Among all Anabaena strains, the maximum activity was determined in Anabaena sp. O-22 (2.26 µl ethylene/mg.h) whereas; the lowest activity was shown in Gloeothece sp. O-Y (0.04 µl ethylene/mg.h). The ma…

Abstract: An actinomycete strain T16-1 demonstrated the maximum poly (L-Lactide)-degrading activity when cultured in basal liquid medium at 50°C. According to 16S rDNA sequence analysis, chemotaxonomic and DNA-DNA hybridization revealed that strain T16-1 belong to the family Thermomonosporaceae, genus Actinomadura. On the basis of phenotypic and phylogenic data, strain T16-1 which is a novel PLA-degrading thermophilic actinomycete was identified as Actinomadura keratinilytica, but the color of its colony on ISP plates, NaCl…

Abstract: In order to study the domestic wastewater influence on secondary metabolite in thyme (Thymus vulgaris L.) under field condition, this experiment was carried out in Iran. In the field, 15 lysimeters having thyme (T. vulgaris L.) were used, where 1 to 5 lysimeters were irrigated by domestic wastewater and primary drainage water was accumulated. 6 to 9 lysimeters were irrigated by primary drainage water and then, were accumulated by secondary drainage water and we have irrigation 10, 11 and 12 lysimeters by secondary…

Abstract: Solid cellulose-based radioactive wastes (CBW) constitutes in some cases, about 70% of the total solid low and intermediate level organic wastes originated from peaceful applications of nuclear technology in various fields of our life. Cesium-137 and cobalt-60 represent two of the most important radioisotopes spiking these waste categories. Both are serious contamination concerns due to their high energy gamma ray emitting (Cs-137 = 0.662 MeV and Co-60 = 1.17 and 1.33 MeV), besides 137Cs is considered as one of lon…

Abstract: A total of 363 Escherichia coli and 224 Salmonella spp. were isolated from diseased chickens during 1993-2008 in China. The susceptibility to eight quinolones and prevalence of plasmid-mediated quinolone resistance (PMQR) determinants was investigated in these isolates. Among the E. coli isolates obtained during 1993-1999, 65.2% were resistant to nalidixic acid, while more than 50% of the E. coli isolates collected during 2000-2008 were resistant to 7 quinolones. All 101 Salmonella spp. isolates obtained during 199…

Abstract: The aim of this study is to examine the antibacterial activity of plant extracts. Both broth dilution and disc diffusion methods were used to assess the antibacterial activity of these extracts against skin and oral pathogens. Eryngium caucaseum Trautv, Eryngium bungei Boiss and Adiantum capillus-veneris have shown antibacterial activity against Streptococcus pyogenes, Streptococcus mutans, Staphylococcus aureus and Streptococcus sanguis. E. caucaseum Trautv showed the highest inhibition zone (> 30 mm) against S. p…

Abstract: The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of cultured Cordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing ace…