Advanced Journal of Microbiology Research

Abstract: Present investigation was the first attempt which deals with the IN VIVO and IN VITRO comparative study of protein level in ACACIA NILOTICA L., a nitrogen fixing tree. Protein was investigated in callus, seed, leaf and stem by means of SDS-PAGE. For obtaining the IN VITRO explant, the seeds were cultured on ½ MS medium under the IN VITRO condition. The cotyledonary nodal explants were taken from the IN VITRO seedlings and cultured in the MS medium supplemented in combination of 2, 4-D (0.4 mg/l) with BAP (0.25 mg/l…

Abstract: Nationally-devised standard treatment guidelines (STGs) for nosocomial infections were evaluated in the context of antibiotic resistance within the public health care system in Kwazulu-Natal. A multi-centre surveillance study instituted in 3 hospitals at 3 progressive levels of health care (district, regional and tertiary) collected consecutive, non-repetitive isolates commonly implicated in nosocomial infections as cited by the STGs, viz., Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Ac…

Abstract: Phytoplankton species composition and seasonal changes were investigated in the shallow reservoir of Adzopé. Taxonomic composition, diversity and abundance of phytoplankton were studied at 4 stations from May 2008 to February 2009, in relation to physical and chemical factors (temperature, conductivity, dissolved oxygen, transparency, pH, nutrients) and climatic factor (rainfall). The phytoplankton comprised 144 taxa, mainly Chlorophyta (29.45% of taxa), Euglenophyta (29.45%) Bacillariophyta (23.97%) and Cyanobacte…

Abstract: Clindamycin is commonly used in treatment of erythromycin resistant Staphylococcus aureus causing skin and soft tissue infections. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to ‘erm’ genes resulting in treatment failure thus necessitating the need to detect such resistance by a rapid method. In the era of automation, vitek-2 system provides a panel for detection of inducible clindamycin resistance with conjunction of other antimicrobial susceptibili…

Abstract: The aim of this study was to determine antibiotic susceptibility of Clostridium difficile isolated from different sources. Two hundred stool samples of staff, patients (at admission to the wards and the same patients after seven days of hospitalization), and 135 samples of hospital environment were collected. Three standard methods including direct plating onto cycloserine-cefoxitin fructose agar, alcohol shock and enrichment culture with 0.1% sodium taurocholate were used to isolate C. difficile. All C. difficile…

Abstract: Rotaviruses are recognized as the most common causes of severe gastroenteritis and death among children worldwide. The aim of this study was to evaluate the disease burden of rotavirus gastroenteritis and the prevalence of different G genotypes of rotaviruses circulating in children aged[...]

Abstract: Bacterial and other pathogens often infect the female genital tract causing disease in most women, sometimes with severe complications. A number of vaginal infections present with few or no symptoms and yet could still be transmissible to sexual partners of infected persons. Poor socio- economic status, lack of diagnostic facilities and shortage of effective treatment all contribute to the high incidence of sexually transmitted and reproductive tract infections. In this study we investigated mixed bacterial and Can…

Abstract: Cytotoxicity and antimicrobial activity of cyclohexane, dichlormethane and methanol extracts of Teucrium scordium subspec. scordioides was studied. Cyclohexane and dichlormethane extracts of T. scordium possessed high citotoxicity against MDA-MB- 361 cells (IC50=130.33±0.1 g/ml and IC50=189.89±3.99 g/ml, respectively). Dichlormethane extract was more effective against MDA-MB-453 cell line (IC50=130.33 ± 0.1 g/ml). The methanol extract of T. scordium possessed no cytotoxicity against breast cancer cell lines, MDA-MB…

Abstract: Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and fungal strains further indicated the high value of this plant species in the medicinal world and could aid in tr…

Abstract: Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35…

Abstract: Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration above 10 g/L inhibited both cell growth and polyhydroxybutyrate (PHB) production. The PHB production by the strain was achieved at the highest PHB content of 52.79% in batch fermentation using the kitchen-waste derived orga…

Abstract: The polarized continuum model (PCM) model has been used to optimize 2,66-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde (safranal) in aqueous phase, methanol and carbon tetra chloride at B3LYP/6-31G** level of theory and the solvent effect has been studied. The principle of maximum hardness has been tested by calculating chemical hardness and chemical potential at B3LYP/6-31G** level of theory to predict the order of stability of the structure in solutions. The maximum IR intensity is related to the C4=C5 stretching…

Abstract: Rotavirus and recently norovirus have been described as important and most common cause of acute gastroenteritis in children. The mode of transmission is fecal-oral. Diagnosis of both of these viruses can be made by rapid antigen detection of the viruses in stool specimens and strains can be further characterized by enzyme immunoassay or reverse transcriptase polymerase chain reaction. Stool specimens collected from various hospitals in Malaysia were examined for norovirus by both immunochromatography and reverse-t…

Abstract: A laboratory study was conducted to investigate efficacy of locally produced microbial inoculants with different concentrations on microbial respiration from soils with and without addition of wheat straw incubated at 20 and 25°C temperatures for 73 days. The results indicated that the soil microbial community structure and activities were obviously influenced by microbial inoculants. In general, whereas all inoculants levels were successfully established to different extents on the straw, none significantly improv…

Abstract: Chromobacterium violaceum, Gram-negative Bacillus, is a common inhabitant of soil and stagnant water found in tropical and subtropical regions of the world. It is a rare cause of severe, often fatal, human disease. In the report, 3 cases of patients infected with C. violaceum were described in Anhui Province, China. Routine and bacteriological investigations were carried out to establish the aetiological diagnosis. Moreover, the patients were treated with appropriate antimicrobial agents and auxiliary therapy. To o…