Advanced Journal of Microbiology Research

Abstract: Medicinal plants contain a variety of chemical substances with important therapeutic properties that can be utilized in the treatment of human diseases. Hydonora africana is used in folklore remedies for the treatment of diarrhoea, dysentery, kidney and bladder complaints among other ailments; hence we assessed the in vitro antimicrobial activity of this plant against three bacterial species (Helicobacter pylori ATCC 43526, Helicobacter pylori PE 252C, Staphylococcus aureus NCTC 6571 and Aeromonas hydrophila ATCC 3…

Abstract: The yeast Malassezia pachydermatis is part of the normal cutaneous microflora of most homoeothermic vertebrates. However, under certain conditions as high humidity, seborrheic skin, corticotherapy and immunodeficiency, it can become pathogenic and cause dermatopathies. The pathogenic role of the genus Malassezia seems to be related to physical, chemical and immunological disturbances and to the production of enzymes, including lipases, phospholipases and hydrolases. The Api- Zym ® system is a semiquantitative metho…

Abstract: Sixty Malaysian CITRUS TRISTEZA virus (CTV) isolates were characterized by bi-directional polymerase chain reaction (BD-PCR) and restriction fragment length polymorphism (RFLP) analysis of their coat protein (CP) gene. In BD-PCR analysis, 392-bp fragments were amplified from seven isolates. The other 53 isolates produced only 320-bp fragments. RFLP patterns of RT-PCR products of CP gene digested with HinfI restriction enzyme were similar to I-IV, VI-VII and two new groups. Isolates AMK1, AMJ12, AMT38, AMT39 and AMT…

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Abstract: Date Palm sap is a fresh juice called “Legmi” widely produced and consumed in Southern Tunisia. The aim of this research was to study microbiological group’s distribution present in 10 palm sap samples, and to select Lactic acid bacteria (LAB) with suitable properties for use as starter cultures in sap lactic fermentation. Microbiological analysis showed high load in fresh palm sap. Mesophilic aerobic bacteria varied from 6.07 to 8.57 log cfu/ml, coliforms ranged from 3 to 6.78 log cfu/ml, yeasts between 3 and 8.47…

Abstract: This study was conducted to determine the prevalence of intestinal parasites in hospitals at Ujjain. A total of 5990 samples were collected in five years: 3580 from Ujjain Charitable Trust Hospital (Urban population) and 2410 from R. D. Gardi Medical College (Rural population). Overall prevalence rate of intestinal parasite was 21.4%. Entamoeba histolytica (10.5%) was the commonest protozoa followed by Giardia lamblia (3.9%). Among the helminths, Ascaris lumbricoides (2.8%) was the commonest. Multiple infections we…

Abstract: Ethnobotanical survey of plants used to treat some common viral diseases, such as chicken pox, poliomyelitis, influenza, hepatitis, measles and jaundice was carried out in Ekiti, Ondo, Osun and Oyo States in South Western Nigeria. Questionnaires were distributed among the participants and oral discussions were employed. Two hundred and eight (208) data were collected and tabulated. The Yoruba names, botanical names, methods of preparation and mode of administration of the plants were considered. The research work s…

Abstract: The study was designed to investigate bactericidal and fungicidal actions of salicid (pH: 6.7, ORP: 760 mV, residual chlorine of 2 to 5 ppm) on hospital infections. Four of the most common opportunistic pathogens (Klebsiella pneumoniae, Staphylococcus aureus, Rhizopus oryzae, and Aspergillus fumigatus) were used for this study. Cultures were inoculated in 9 mL of salicid and incubated for 0.5, 1, 3, 5, 7 and 10 min at room temperature (23 ± 2°C). A dipping method was followed for this study. Untreated pathogens wer…

Abstract: Complete and balanced nutrition with reliable food consists of the basis of health and protective health services. Therefore, the current study was carried out to determine the knowledge of food safety level and purchasing behavior of 668 consumers living both in Turkey (n=348) and in Kazakhstan (n=320) and to compare the results. Volunteered consumers for the research were given a face to face interview between March and September 2010. It was found that the knowledge of purchasing behavior (14.43±2.56) of food sa…

Abstract: Infections caused by ESBL producing members of the enterobacteriaceae have rapidly increased all over the world. ESBL increase the possibility of failure of empiric antimicrobial regimens. The aim of this study was to determine the prevalence of ESBL in bacterial isolates and to look into the options for treating infections caused by these organisms. A total of 4,150 isolates of enterobacteriaceae were studied. ESBL producer isolates were 371 (8.94%) out of which 281 (75.7%) were recovered from admitted patients wh…

Abstract: This survey was carried out to determine the seroprevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) infections in commercial layer farms in Centernorth of Iran. A total of 2000 serum samples were collected from 40 commercial layer flocks (50 samples/ flock) mostly > 40-week-old. Sera tested by serum plate agglutination (SPA) method using commercial MG antigen and MS antigen. Positive reactions retested by SPA on 1:8 dilution and the flocks with more than 10% positive reactions considered posit…

Abstract: The aim of this study was to explore the killing kinetics and bactericidal mechanism of action of A. galanga against food borne bacteria in order to promote this plant as a source of natural preservative. The comparison on antibacterial power was firstly done among its essential oil and the crude extracts obtained from various extracting solvents. The essential oil showed the extremely strongest antibacterial activity and more effective killing activity against Gram negative than Gram positive food borne bacteria…

Abstract: A bacterium producing agarase was isolated from the vellar estuary and potential strain was identified as Bacillus subtilis. The ideal parameters was found to be of pH 8, temperature of 40°C and 3% of NaCl concentration for optimal growth and agarase activity. Agar as carbon source and yeast extract as nitrogen source was found to be suitable for optimum growth and agarase activity. The activity of enzyme obtained from the cell free filtrate was 15.2 U/L and the activity of the partially purified enzyme was 5.3 U/L…

Abstract: The prevalence of typhoid fever in referral hospitals in Umuahia and Aba, Abia State, Nigeria was studied using standard microbiological techniques. A total of 500 subjects within the age range of 1-70 years were enrolled. A total of 229 subjects were infected with a prevalence rate of 45.8%. The males had the highest prevalence rate of 50.9%, while females had a prevalence rate of 41.9%. In Aba, 125 (50.0%) subjects were positive, while 104 (41.6%) were recorded in Umuahia. The age group of 21-30 years had the hig…

Abstract: The objective of the study was to evaluate mesophyle, psychrotroph and somatic cell counts (SCC), and presence of Staphylococcus aureus in raw milk produced in 42 dairy farms of the State of São Paulo. Farms were classified according to milk yield in low (< 400 L/day, n = 17), intermediate (400 - 1,000 L/day, n = 8) and high milk production (> 1,000 L/day, n = 17). Mesophylic bacteria counts were lower (p < 0.05) in high production farms, with no differences (p > 0.05) in psychrotrophic and somatic cell counts betw…