Advanced Journal of Microbiology Research

Abstract: Enzymes categorized under the 2OG-Fe (II) oxygenase superfamily and Taurine catabolism dioxygenase TauD family demonstrated a striking structural conserveness even with low protein sequence homology. It is evident that these enzymes have an architecturally similar to catalytic centre with active ligands lining the reactive pocket. Deacetoxycephalosporin C synthase (DAOCS), isopenicillin N synthase (IPNS), deacetylcephalosporin C synthase (DACS), clavaminate synthase 1 and 2 (CAS1 and 2) are important bacterial enzy…

Abstract: Hop stunt viroid (HSVd) variants from cachexia symptomatic citrus tree were subjected to retro-transcription and DNA amplification (RT-PCR), cloning and sequencing. Here we report genetic diversity and phylogenetic analysis of HSVd Tunisian isolate. Our study revealed obvious polymorphism within Tunisian isolates and high similarity with Japanese variants. Neighbor-joining analysis was carried out on the new HSVd-citrus sequences together with 44 previously described HSVd isolates from citrus and one from grapevine…

Abstract: The most common surface structures bacteria are monomolecular crystalline arrays of proteinaceous subunits termed surface layers or S-layers. Since S-layer-carrying organisms are ubiquitous in the biosphere and because S-layers represent one of the most abundant cellular proteins, it is now obvious that these metabolically expensive products must provide the organisms with an advantage of selection in very different habitats. S-layers have been associated with a number of possible functions that relate to pathogeni…

Abstract: In a previous experiment, sweetpotato whitefly was found to be difficult to control with either neem or Beauveria bassiana alone. This earlier research also reported that the combined application of neem and B. bassiana increased mortality of whitefly nymphs when both of these products were applied to eggplant foliage; but a higher concentration of neem (more than 0.5%) was slightly toxic for young eggplant. Therefore, the present research was undertaken with these two products in which neem was applied as soil dre…

Abstract: The X-VP agar, chromogenic selective medium for Vibrio parahaemolyticus, was evaluated for inclusivity and exclusivity by using 169 strains including 55 V. parahaemolyticus and compared with Thiosulfate Citrate Bile salt Sucrose (TCBS) and CHROMagar Vibrio media as conventional methods using V. parahaemolyticus inoculated seafood samples. Our results suggested the X-VP agar was useful for isolation and enumeration of V. parahaemolyticus.[...]

Abstract: Yeast infections of the vagina are more prevalence and frequently recurring problem of women. Such infections are caused primarily by Candida albicans and apparently occur when the environmental balance in the-vagina is disturbed. Lactobacillus acidopbilus restricts the growth of pathogenic microorganisms per se displayingwide inhibitory spectrum substances. Fifteen (30%) C. albicans isolates were obtained through 50 vaginal swabs from patients who visited the obstetrics and Gynecology clinic departments of hospita…

Abstract: Aqueous (cold and hot) and ethanol extracts solutions of the Henna plant (Lawsonia inamis) leaves was adapted for the first time as a counter stain in Gram staining reaction. Different extracts of L. inamis leaves were formulated into various staining solutions of different concentrations and modified with hydrogen peroxide, ferric chloride, potassium alum and potassium permanganate. These staining solutions were used to stain both known Gram positive and Gram negative bacterial isolates using Gram staining techniq…

Abstract: Five pseudoguaianolide sesquiterpenes, namely neoambrosin, damsinic acid, damsin, ambrosin and hymenin isolated from the aerial parts of Ambrosia maritima, were tested for their antifungal activity against the most economic plant pathogenic fungi Botrytis cinerea and Fusarium oxysporum which cause grey mold and root rot diseases, respectively. In mycelial radial growth inhibition assay, neoambrosin and damsin were the most potent, while hymenin and damsinic acid were the lowest effective among the tested compounds…

Abstract: Supplementations of formulae with synbiotic compounds of human milk have shown to be able to decrease several gut-related diseases in formula fed infants. This study was carried out to develop a synergistic cryoprotective drying medium for infant formulae probiotic application. Response surface methodology (RSM) was employed to optimize the concentrations of skim milk and prebiotics for improvement of the cell survival of Bifidobacterium infantis 20088, during freeze-drying. The optimal composition was found to be…

Abstract: The efficiency of urea is poor due to the substantial amount of N lost to the atmosphere by ammonia volatilization. Thus, the objectives of this greenhouse study were to evaluate the effectiveness of mixing urea with zeolite and sago waste water on N, P and K uptake and their use efficiency in maize cultivation, and soil exchangeable ammonium, available nitrate, pH, exchangeable K, and available P contents. The treatments evaluated were: No fertilizer (T0), 2.02 g urea (T1), 2.02 g urea + 30 g zeolite + 7 L sago wa…

Abstract: In this study, it was found that supplementation of 0.6 g/L rutin could improve the fermentation performance of Ganoderma applanatum ACCC-52297 in terms of mycelial growth and ganoderic acid (GA) production. Albeit retarded mycelial growth was detected at the first 36 h in the submerged culture of G. applanatum ACCC-52297 with the addition of rutin, kinetic model analysis revealed that rutin could improve mycelial growth and GA synthesis at the later phase of the fermentation (after 36 h). As such, rutin post-feedi…

Abstract: In 1880, Staphylococcus aureus was first discovered by a surgeon named Sir Clifton Smithin pus from surgical abscesses in Aberdeen, Scotland. Methicillin, as the first beta-lactamase resistant penicillin, was used to treat S. aureus infection in 1961. The first methicillin-resistant Staphylococcus aureus (MRSA) was identified in the United Kingdom in the same year. It appeared in the United States in 1981 among intravenous drug users. MRSA is an important agent of hospital-acquired infection. Two hundred patients w…

Abstract: a-Glucosidase enzymes are responsible for hydrolyses of carbohydrates thereby converting dietary disaccharides into absorbable monosaccharide. Inhibitors of such enzymes can be used as clinical tool for treating diabetes. Conventionally such inhibitors are reported from plant and microbial sources. However, microbial inhibitors are considered to be the most important as they are capable of producing micro molecular compounds. Hence the present study was conducted to test actinobacteria for their ability to produce…

Abstract: Ninety six samples of water collected from Dal Lake, Kashmir at eight different sites for a period of one year from April 2010 to March 2011 were tested for the presence of Penicillium species. Five different species of the said genus were isolated from the samples by serial dilution technique. Out of total 122 fungal colonies isolated, five species of Penicillium viz, Penicillium olivicolor, Penicillium commune, Penicillium chrysogenum, Penicillium funiculosum and Penicillium dimorphosporum were obtained. Among th…

Abstract: Bacteremia due to Acinetobacter baumannii is a common problem in hospitals worldwide. Physicians in the intensive care units (ICUs) encountered a serious challenge of finding a drug to cure the extended-spectrum beta-lactamase (ESBL) producing and multi-drug resistant (MDR) A. baumannii bacteremia. Our aim was to determine the frequency of A. baumannii bacteremia in an Iranian hospital ICUs, their antibiotic susceptibility patterns, and the frequency of ESBLs by a cross-sectional study. A total of 340 patients admi…