Advanced Journal of Microbiology Research

Abstract: Lipase enzyme is an important group of biocatalyst for biotechnological application. One of the major industrial microorganism is the Bacillus species which are in abundance in the hot springs. The aim of this research was to isolate and characterize lipase producing bacteria from five hot springs in Malaysia. Eight bacterial strains from five hot springs were characterized by both biochemical and molecular techniques. Four strains were lipase producing thermophilic bacteria (coded as A1, A3, A4 and A14). The prese…

Abstract: Drug resistance is widely distributed in Escherichia coli coli, and related technology is urgently required to control its transmission. The Chinese pulsatilla extracts containing crude drug of 12.5 mg/ml was used to eliminate resistance plasmid against streptomycin in chicken E. coli. After screening and identification, plasmids were extracted from resistance-eliminated strain and electrophoresed in order to compare plasmid profiles before and after elimination. After the drug resistance was eliminated, the diamet…

Abstract: Endophytic Fusarium species were isolated from roots of wild banana (Musa acuminata) collected randomly in several locations in Penang Island, Malaysia. A total of 54 isolates of Fusarium were recovered from 100 root fragments. Based on morphological features of macroconidia, microconidia and conidiogenous cells, three Fusarium species were identified in which the most common species was F. oxysporum (41.5%) followed by F. solani (32.1%) and F. semitectum (24.5%). The present study showed that there are diverse end…

Abstract: The purpose of the present study was to investigate the current microbiological spectrum of culture-positive explanted scleral buckles and their sensitivity to antibiotics. A retrospective, consecutive case series was performed in this study. 38 patients with scleral buckle removal that occurred between January 2003 and December 2010 were retrospectively analyzed. Cultured bacteria were identified using the API system. Susceptibilities of the bacteria were tested using the Kirby-Bauer disk diffusion method. 38 of 5…

Abstract: Although many factors that affect the frequency of natural transformation of the unicellular cyanobacterium Synechocystis have been extensively reported, little is known regarding the effects of changes in the spectral quality and intensity of light on its natural transformation. The frequency of natural transformation was significantly enhanced or inhibited by the treatments with low light (LL) or high light (HL) under the incubation condition of cells and DNA before plating and/or on the plates in comparison with…

Abstract: The aim of this study was to evaluate the antimicrobial efficacy of Mirabilis jalapa and Dichrotachys cinerea against biofilm and extended spectrum of beta lactamase (ESBL) producing uropathogenic Escherichia coli (UPEC). M. jalapa and D. cinerea are widespread medicinal plants traditionally used to treat infectious diseases. Aqueous, acetone and ethanol extracts of leaves of M. jalapa and D. cinerea were tested for antimicrobial activity invitro by the agar well diffusion method. Ethanol extract of M. jalapa leave…

Abstract: Klebsiella pneumoniae MTCC 7162, isolated from tannery effluent was used for the production of extracellular tannase. Central composite design (for nutritional parameters) and Box Behnken design (for physicochemical parameters) were used to arrive at conditions for maximal tannase production. Box Behnken design was statistically significant and indicated that pH is a significant factor for tannase production. A 6.5 fold increase of extra cellular tannase (22.7 U / ml) production was observed at the optimized condit…

Abstract: Avibacterium paragallinarum causes a highly contagious disease in chickens called infectious coryza. We have previously isolated A. paragallinarum from diseased chickens and demonstrated their virulence in experimental infection in different poultry species. In the present study, the isolates were screened for selected resistance genes for clinically relevant antibiotics. The study demonstrated resistance genes for sulphamethoxazole, tetracycline, streptomycin and ampicillin resistance in the isolates. Multiple res…

Abstract: Fungal diseases of fresh water eggs and fishes are known to be a problematic disease. The aim of this study was to investigate the aquatic fungal flora specially oomycete fungi in rainbow trout hatcheries from west part of Iran during fall and winter 2008, an attempt to isolate fungi from the eggs and broodstocks showing fungal infection at the three rainbow trout farms. A total of 150 randomly selected eggs and 15 broodstocks were obtained. A sterile swab was taken from outer surface of body (skin, gills, fins), a…

Abstract: The extracts of Eucalyptus camaldulensis Dehnh. and Eucalyptus torelliana F. Muell. (Myrtaceae) were screened against four non-tuberculous mycobacteria species: Mycobacterium fortuitum ATCC 684, Mycobacterium smegmatis ATCC 19420, Mycobacterium phlei ATCC 19240 and Mycobacterium abscessus. The agar diffusion method was used to investigate the activity of these plants at 1 and 2 mg/ml concentration. The methanol extracts exhibited the highest activity against the test organisms, the most susceptible being M. fortuit…

Abstract: The pomegranate, Punica granatum L., is an ancient, mystical, unique fruit borne on a small, long-living tree cultivated throughout the Mediterranean region. Pomegranate is used in several systems of medicine for a variety of ailments. The synergistic action of the pomegranate constituents appears to be superior to that of single constituents. P. garantum, have been reported to have antimicrobial activity against a range of Gram positive and negative bacteria. Pomegranate formulations containing ferrous salts have…

Abstract: We aimed to delineate the incidence of hepatitis C virus (HCV) infection and HCV seroconversion (SC) in maintenance hemodialysis (HD) patients and to evaluate the effect of isolation measures on HCV in HD unit. From June 1998 to June 2010, 2465 maintenance HD patients in our HD unit were enrolled in, and the anti-HCV ELISA and HCV nucleic acid testing were consecutively performed every six months. The results showed the prevalence rates of HCV antibody detected consecutively every six months were 54.7, 53.8, 52.6…

Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial and community infections. It has shown resistance to most of the currently available antibiotics and nicknamed ‘super bug’. Anti-staphylococcal activities of tannic acid, quercetin and gallic acid ethyl ester in combination with fusidic acid and rifampicin were determined against five strains of S. aureus, including three clinical strains. Tannic acid and quercetin were found to be synergistic with fusidic acid and rifampici…

Abstract: Homopolysaccharides produced from sucrose by lactic acid bacteria (LAB) are of interest as natural additives to improve textural properties of foods. The production of exopolysaccharide (EPS) by WEISSELLA strains, which had been isolated from a traditional Thai food (plasom), was investigated using sugarcane molasses and white sugar from sugarcane as substrates in comparison to analytical grade sucrose. EPS production was evaluated at 30°C for 48 h. EPS production and bacterial growth were influenced by the type of…

Abstract: The epidemiology of Entamoeba histolytica infection in Sudan is poorly understood. This is due to the inability to differentiate E. histolytica from the non pathogenic, Entamoeba dispar. Old methods used such as direct microscopy and culturing are insensitive compared to polymerase chain reaction (PCR). In this study, light microscopy and PCR were utilized to study the prevalence of Entamoeba infection in patient attending University of Medical Science and Technology (UMST) hospital, in Khartoum, Sudan. By microsco…