Advanced Journal of Microbiology Research

Abstract: A total eighty four epidemiologically unrelated clinical isolates of Salmonella enterica serovars were subjected to antimicrobial susceptibility testing and molecular detection of class 1 and 2 integrons. Eleven isolates (13.1%) which were resistant to at least 4 groups of antimicrobial agents considered as MDR (multidrug resistant) Salmonella serovars. PCR assays detected intI1 and intI2 genes in 50 (59.5%) and 14 (16.7%) of Salmonella clinical isolates respectively. Emergence of MDR Salmonella serovars demonstrat…

Abstract: Ustilago maydis FBD12 is a phytopathogenic fungus that grows in submerged environments and produces different metabolites, such as glycolipids (biosurfanctants), cyclic peptides, tryptophan derivatives, and enzymes. Biosurfactants are molecules with a high surface activity, as well as emulsifying properties. They are secondary metabolites whose main physiological role is to support the growth of microorganisms in water-immiscible substrates, through the reduction of the superficial tension of the interface. In the…

Abstract: Total RNA of Eimeria tenella drug-resistant strain from Tangshan was extracted with Trizol. Differential-display reverse transcription- polymerase chain reaction (DDRT-PCR) was established by 3 anchored primers and 20 arbitrary primers. The products of polymerase chain reaction (PCR) were analyzed on the denaturing polyacrylamide gels by silver-straining. Ten differential bands were excised from the gels and reampilfied with the same sets of primers. The products were purified and ligated with PMDTM18-T Vector, and…

Abstract: The present study is aimed to investigate Salmonella species in food of animal origin collected from Riyadh, King Saudi Arabia (KSA) using conventional methods and polymerase chain reaction (PCR), targeting fimA gene specific for members of genus Salmonella. Salmonella isolation revealed 20 Salmonella serovars (8%) out of 250 examined samples. Nine strains (6, 92%) were recovered from 130 examined minced meats and 5 Salmonella strains (8.33%) were recovered from 60 local frozen chickens. Moreover, 6 Salmonella stra…

Abstract: We describle a suspicious Meningococcal death case, confirmating by the laboratory PCR methods. The infection developed rapidly within only 24 h until the patient died. Analyzing the close contact strain and the DNA template extracted from the serum of this patient, we characterized a serogroup B Neisseria meningitidis as the pathogen of this case, which, in terms of sequence typing, belonged to ST-4821 complex which first characterized in serogroup C Neisseria meningitidis as a new unique hypervirulent meningococc…

Abstract: Newly isolated fungi, strain Tolypocladium cylindrosporum syzx4 from rotten corn stover, can effectively produced extracellular thermoacidophilic β-glucosidase (syzx4) using agro-industrial residues. After purification and characterization, the β-glucosidase was applied to saccharify steam explosion pretreated corn stover (SCS) with commercial celluclast from Trichoderma reesei when compared with the β-glucosidase (Novo-188) from Aspergillus niger supplementation. Based on the results of the single factor tests, fu…

Abstract: We investigated the relationship between fixed orthodontic appliances and oral Candida colonization. The influence of some important nutritional and salivary factors was also investigated. These factors included: hemoglobin, vitamin B12, ferritin and folate levels, salivary flow rate, salivary pH, tobacco smoking and dietary habits. Patients enrolled in this study were adolescent patients aged 12- 18 years who attended the Orthodontics Department/University of Jordan Hospital for the provision of fixed metallic ort…

Abstract: Salinity of soil is a serious problem affecting plant growth and is increasing steadily in many parts of the world, particularly in arid and semi-arid areas. Arbuscular mycorrhiza (A M) is the most wide spread and significant mutualistic fungi having universal in their association including plants of agricultural and medicinal importance. A. mycorrhiza fungi have been shown to promote plant growth and salinity tolerance by various mechanisms. The effects of inoculation with two A. mycorrhizal fungi Glomus mosseae a…

Abstract: Bacteriocins are of special interest due to their potential value as natural preservative. The present study is a trial for production of such bacteriocin from a marine Lactobacillus sp. Lactic acid bacteria (LBA) was isolated from fish gut (Mugil cephalus) and prawn muscle (Peneaus monodon) samples and their density were found to be 5.2 x 107 and 6.4 x 107 CFU/g respectively. Various pathogens were isolated from ready to eat pickle samples (bottled). The LAB strains were tested against 10 different commercial anti…

Abstract: To improve the yield of exopolysaccharides (EPS) by Bacillus thuringiensis 27 from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China and to analyze its bioflocculant activity, orthogonal matrix method was used and this method enabled us to obtain maximum EPS production. By studying the optimal medium condition of beef extract 3 g/l, peptone 10 g/l, maltose 40 g/l, and NaCl 4g/l we observed that the optimal medium condition was pH 6.0, incolum size 8%, liquid volume 40 ml in 200 ml flak and tempe…

Abstract: The activity of 13 antimicrobials was studied, to determine multiple antimicrobial resistances, against 208 Pseudomonas strains (125 Pseudomonas sp and 83 Pseudomonas aeruginosa) isolated from river and aquaculture samples. Isolates were confirmed with API 20 NE. Isolates from both sources reported high resistance rates for similar antibiotics. These were: Ampicillin (100, 100%), cefuroxime (96.8, 81.9%), streptomycin (93.6, 78.6%), cefotaxime (93.6, 77.1%), for river water and aquaculture samples respectively. Whi…

Abstract: This study aims at determining the seroprevalance of Coxiella burnetii in cows, sheep, goats and staff, working in the stock breeding sector in Diyarbakir region. Therefore, C. burnetii antibodies were investigated in sera samples of 612 sheep, 700 goats, 584 cows and 90 staff by enzyme-linked immunosorbent assays (ELISA). In the study, seropositivity was obtained as 25.4, 38.6, 20.0 and 6.6% in sheep, goats, cows and stockbreeding staff, respectively. Consequently, C. burnetii seropositivity, whether in people or…

Abstract: Gamma rays mediated mutagenesis and subsequent selection on 2-deoxy-D-glucose developed a highly catabolite de-repressed and stable β-glucosidase (BGL) hyper-producer mutant of local Aspergillus niger NIBGE, which has high potential for industrial applications. The M6 mutant showed highest constitutive production of BGL (14,900 IU/L) on glucose as compared to control (5,900 IU/L). Wheat bran was the best carbon source and BGL production by parent and mutant strain was 16,200 and 29,500 IU/L, respectively. Effect of…

Abstract: By differential proteomics analysis, it was found that the expression level of IDH3 (Isocitrate dehydrogenase 3 ) in mouse liver was significantly increased under the stress of Aflatoxin B1. To validate the result of differential proteomics analysis, fluorescence quantitative PCR was used to detect the changing trend of idh3 mRNA volume induced by Aflatoxin B1 in mouse liver. The result showed that the expression volume of idh3 mRNA showed an increasing trend with the increase of Aflatoxin B1 concentration, which c…

Abstract: The aim of this study was to investigate the inflammation genes (IL-1 , IL-6, TNF - and iNos) responses to Plasmodium chabaudi malaria in the liver of female C57BL/6 hepatocytes via mRNA expression due to infection with P. chabaudi at different time points. Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then scarified at days (0, 1, 4 and 8 respectively). RT-PCR was used to quantify liver inflammation genes. The levels of IL-1 , IL- 6 and TNF were significantly increased at…