Advanced Journal of Microbiology Research

Abstract: The demand for fermented dairies is on the increase in developing countries following consumer awareness of some of the health benefits attributed to prolonged intake. Eight samples of yoghurt produced from Enugu, Eastern Nigeria was collected from various locations. Samples were incubated using various media for the isolation, identification and enumeration of the bacteria population within the yoghurt. Reports indicated that total viable count of bacteria was in the range of 1.4 x 106 – 2.2 x 107 cfu /ml. Not all…

Abstract: Flow cytometry’s (FCM) measurement of membrane potential (MP) and cell respiration viability based on continuous culture was used to investigate the responses of aerobic anoxygenic phototrophic bacteria (AAPB) in the heterotrophic growth and regulation mechanism of photosynthesis to environmental changes. An AAPB strain Erythrobacter sp. JL475 and a non-AAPB strain Erythrobacter sp. JL316 were used as the experimental bacteria, both of which were isolated from the South China Sea. The results showed that light -cul…

Abstract: Dihydrodipicolinate synthase (DHDPS) catalyses the first enzymatic step in the lysine-biosynthetic pathway in bacteria, plants and some fungi. In this study, cloning and submission of 3639 bp from Streptomyces rimosus NRRL 2455 to the EMBL database under the accession code EU617017 were carried out. The entire dprA gene was cloned and heterologously expressed in Escherichia coli BL21 (DE3) and the gene product was purified and analysed spectrophotometrically. A dprA- knock-out mutant was created and showed a reduct…

Abstract: Lignocellulosic biowastes can be utilized to produce microdiesel, a promising alternative energy source for limited crude oil and food based biofuels. As an alternative fuel, microdiesel produced from biowastes it has superior environmental benefits, economically competitive, producible in sufficient quantities and the energy conversion efficiency is also estimated in the article in theory. Microdiesel derived from biowastes is a potential renewable and carbon neutral alternative to petroleum fuels. Unfortunately…

Abstract: Aeromonas hydrophila is an opportunistic pathogen of aquatic and terrestrial animals and human beings. The aim of this study is to investigate 25 isolates of A. hydrophila from 40 fish muscles and 12 water samples. All isolates are screened for the presence of virulence factors such as aerolysin, hemolysin and lipase both phenotypically as well as genotypically. All the isolates produced lipase, whereas only 60% of isolates produced b-hemolysis with RBCs. The presence of 3 virulence genes aerolysin, hemolysin and l…

Abstract: Microorganisms and their natural products are potentially important for the biological control of crop diseases without detrimental effects to the environment. In this study, acetonitrile-methanol extracts of 361 actinobacterial isolates obtained from Aberdares, Arabuko Sokoke, Lake Bogoria, Mt Kenya, Kakamega, Ruma, Shimba Hills and Imenti forest national parks in Kenya were screened for antagonism against Fusarium oxysporum, Fusarium spp and Colletotrichum kahawae, which are important crop pathogens. Twenty-three…

Abstract: Protein phosphorylation on tyrosine has been demonstrated to occur in a wide array of bacterial species and appears to be ubiquitous among prokaryotes. In Deinococcus radiodurans, after the predicted protein-tyrosine phosphatase (PTP) gene DR2161 was deleted, the radiation resistance of the bacterium changed a little. The natural resistance-associated macrophage protein gene DR1709 was the possible target of PTP. But the radio resistance of the double mutant (DR1709 and DR2161 that were deleted) was almost the same…

Abstract: The red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in Escherichia coli K-12 through homologous recombination using linear PCR products. Here, E. coli BW 25113 mutants were created by changing the localization of genes on the genome using a method which contains both inactivation and reinsertion steps. The phoA gene, the structural gene for alkaline phosphatase, was inactivated by having the ORF and its regulator region replaced with a kanamycin cassette flanked by FLP r…

Abstract: Antibacterial and antifungal activity of the acetone, methanol and aqueous extracts of the lichen Lecanora frustulosa and Parmeliopsis hyperopta and their divaricatic acid and zeorin constituents has been screened in vitro against the following species of microorganisms: Bacillus mycoides, Bacillus subtilis, Staphylococcus aureus, Enterobacter cloaceae, Escerichia coli, Klebsiella pneumoniae, Aspergillus flavus, Aspergillus fumigatus, Botrytis cinerea, Candida albicans, Fusarium oxysporum, Mucor mucedo, Paecilomyce…

Abstract: The hydroethanolic extracts obtained from ten plant species being used as components of skin disease remedy in Southwest Nigeria were subjected to phytochemical analysis and screened for antimicrobial activity. The antimicrobial activity was determined against Escherichia coli NCIB 86, Staphylococcus aureus NCIB 8588, Klebsiella pneumoniae NCIB 418, Pseudomonas aeruginosa NCIB 950, Proteus vulgaris NCIB 67, Bacillus subtilis NCIB 3610, Candida albicans and Aspergillus flavus by agar diffusion method. Terpenes, flav…

Abstract: Malaria infection generates oxidative stress which has serious effect on the haemoglobin (Hb) level of the infected individuals. This work studied the effect of blood group, age and gender on prevalence of malaria, oxidative stress and Hb level. 5 ml of blood samples were collected into EDTA bottle from 120 volunteered adult males and non-pregnant females. The plasma was separated and used to quantify oxidative stress by measuring malonaldehyde (MDA) and superoxide dismutase (SOD) levels using colorimetric method…

Abstract: Naturally occurring yeasts were sought from diverse Tanzanian environments and screened for industrial application. The yeasts were isolated from environments such as traditional brews and wines from various parts of Tanzania. In this regard, a total of thirty yeast isolates were screened for their suitability in Industrial Very High Gravity Fermentation (VHGF). Five of these isolates were able to grow and ferment medium with 40% initial sucrose concentration. Whereby, three were able to grow and ferment medium wit…

Abstract: To determine current trends of antibiotic resistance among clinically significant pseudomonas aeruginosa strains causing various nosocomial and community acquired infections. A total of 100 clinical isolates of P. aeruginosa from inpatient and outpatient were studied from June 2008 to May 2009 for its prevalence and susceptibility profiles. Most of the isolates were from pus followed by urine, sputum, blood, ear swab and catheter tip. Uropathogenic P. aeruginosa infections were higher in females than males, ratio w…

Abstract: This study aimed to evaluate the protective effects of sucralfate on immunoglobulin yolk (IgY) and to provide a theory basis for preparing IgY oral preparations which can tolerate acid and pepsin. Engineering bacteria DH5 -vacA-pQE30 were largely induced to express recombinant vacuolate cytotoxin antigen (VacA). VacA antigen was inoculated in Lohman hen and IgY was purified. Different concentrations of sucralfate were added into IgY solution including different concentrations of pepsin at different pH values. IgY s…

Abstract: In this study, the effect of sugars type (glucose, sucrose, fructose and the mixture of glucose and fructose) and best substrate concentration are investigated on biofilm accumulation. Then, the role of this substrate examined on gtfB expression level by real time RT PCR. A total of 40 Streptococci strains isolated from dental caries and plaque of patients. The ability of them to form biofilm measured in the presence of some sugars on micro titer plates. The mean of resulted optical density of formed biofilm in the…