Advanced Journal of Microbiology Research

Abstract: A study of the antibiotic resistance pattern of Staphylococcus aureus from the nasal cavities of patients with pneumonia attending the University of Benin Teaching Hospital, Benin City, Nigeria was conducted. The survey revealed S. aureus to be the most predominant cause of Staphylococcal pneumonia with an occurrence rate of 38.5%. Other microorganisms encountered included Staphylococcus epididermis, Streptococcus viridans and Brahamella catarrhalis. Patients in age group 1 - 5 years had the highest incidence rate…

Abstract: Anti-listerial effect of freeze dried fractions of partially purified bacteriocin (Fd-PPL) and the producer strain Lactobacillus casei RN 78 strain was evaluated in experimental cheese samples during storage at two different temperatures (4 and 35˚C). With the addition of 6400 AU/g of Fd- PPL fractions to the cheese samples of the initial concentration of 4.81 +/- 0.06 log CFU/ml of Listeria monocytogenes was reduced up to 0.91 +/- 0.01 log CFU/ml. Whilst, in the presence of 107 CFU/ml live bacterial cells of L. ca…

Abstract: Herpes simplex virus type 2 (HSV-2) is a member of herpesviridae family and subfamily of alphaherpesvirinae. HSV-2 infection often causes genital herpes in women and men, abortion, infant herpes and non-infectious meningitides. Glycoprotein D and B, respectively, are coded by gD and gB gene. They are the adhesion of HSV-2 attached to the surface of the epithelial cells and are used for making vaccine. To determine the phylogenetic analysis of HSV-2 gD gene and cloning of it in E. coli, infected samples from Esfahan…

Abstract: Mastitis continues to be the most economically important disease of dairy cattle, and current data on mastitis is even less readily available. To investigate into the prevalence of the pathogens responsible for bovine mastitis, 100 raw milk samples were obtained from the dairy cows with clinical or sub-clinical bovine mastitis in 5 farms in Jiangsu Province, China. All the samples were submitted to bacterial isolation and identification by morphologic examination and biotyping. The data revealed that Escherichia co…

Abstract: Alterations of serum copper concentrations are commonly found in patients suffering from gastrointestinal infections and with hepatic, renal, cardiovascular and malignant diseases. In this study, we aimed to investigate the changes of total content of the essential element of copper level in patients infected with Giardia intestinalis. Copper concentration was measured in 64 patients who were positive for intestinal parasite of G. intestinalis. Scores obtained for the positive samples, their age and sex, matched 60…

Abstract: Toxoplasmosis is a parasitic disease prevalent all over the world and it’s causal agent is Toxoplasma gondii. Toxoplasmosis is very important in pregnant women. Seroepidemiological survey of toxoplasmosis in young girls before marriage for identifying non immune girls could be used to prevent congenital toxoplasmosis. A seroepidemiological study of toxoplasma IgG-antibody in 516 high school girls was conducted. Sample cases were chosen randomly from public high school girls. Blood samples were collected and the obt…

Abstract: Eighteen fungal endophytes strains parasitizing the famous Chinese medicinal plant Paris polyphylla Smith var. yunnanensis (Franch.) Hand. Mazz were isolated from surface-sterilized plant tissues, such as rhizome, root, stem, leaf and flower. The effect of endophytes on the growth of human pathogenic microbes was evaluated in vitro, using disc diffusion assay. According to the characteristics of cultures and DNA sequences, the WRF7 and WRF7’ belonged to the species Penicillium chrysogenum. The extract of WRF7’ inhi…

Abstract: The biocontrol potential of Bacillus subtilis S499 was evaluated on tomato in open field sites in low altitude area of the plain of Imbo in Burundi. This strain was tested in order to reduce the impact of an important fungal disease giving rise to large losses in local plantings. The causing pathogen was isolated from diseased leaves at different locations in the fields and identified as Fusarium most probably related to the semitectum species according to the fermentation profile, morphology and gene homology. Res…

Abstract: Production and partial purification of protease enzyme by Bacillus laterosporus was the aim of this study. B. laterosporus was allowed to grow in shake flask broth culture for purpose of inducing protease enzyme. The protease enzyme was purified by ammonium sulfate precipitation followed by dialysis and further concentrated by Amicon tubes. After concentration, the protein was subjected to 12% Zymogram gel with gelatin and the molecular weight of the protease enzyme was 15 kDa. The protease activity increased as th…

Abstract: The chemical composition and the antibacterial activity of the essential oil of Mentha longifolia L. ssp longifolia are being investigated in the present work. The minimal inhibitory concentration (MIC) of this oil against four gram+ and gram- reference bacteria including Salmonella typhimurium LT2, Escherichia coli ATCC 35218, Microccus luteus NCIMB 8166 and Staphylococcus aureus ATCC 25923 was used to study the morphological alteration of bacterial wall cell visualized by atomic force microscopy (AFM). The chemic…

Abstract: In this work, the cell growth and ethanol productivity was tested for lignocellulosic biomass sugars of overliming-detoxified and NaOH-neutralized rice straw acid hydrolysate by using Candida shehatae CICC 1766. When the acetic acid reached 3 g/L in defined xylose medium, the final ethanol concentration was 33% of that in the medium without acetic acid addition. C. shehatae CICC 1766 could bear lower pH (ethanol yield 13.5 g/L at pH 4.0) in overliming-detoxified hydrolysate. At the optimal pH 5.0 the ethanol yield…

Abstract: Fresh white bread and ‘eba’ (a carbohydrate; > 80% starch, indigenous staple food in southern Nigeria) were purchased from traders in Lagos, Nigeria and stored under aseptic conditions in the laboratory at room temperature (27 ± 2°C) (11 h of darkness and 15 h of daylight) for duration of 144 h, during which samples were analyzed at 48 h intervals for fungal population using the plate count technique. The sensitivity of the spoilage fungi to aqueous and methanol extracts of leaves of Thaumatococcus danielli and Meg…

Abstract: Genetic diversity of Fusarium oxysporum f. sp. cubense (FOC) isolates were analyzed using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), random amplified microsatellites (RAMS) and restriction fragment length polymorphism of Intergenic Spacer (RFLP-IGS) . The three molecular techniques showed intraspecific variations and the banding patterns generated using each technique were highly variable. UPGMA cluster analysis of the combined data of the three techniques showed 35.7 - 100% genetic similarity…

Abstract: Five spore-forming bacteria producer of lactic acid were isolated from soils sampled in the vicinity of poultry farms in Burkina Faso. All isolates were Gram-positive, motile, mesophilic, facultative anaerobic, catalase positive rods, and with L(+) lactic acid production. The isolates have been characterized and identified by a polyphasic approach, combining various phenotypic and genetic characteristics. The 16S-rDNA-sequence analyses revealed the membership of two isolates to the genus Bacillus and the three othe…

Abstract: To learn the antifungal effects of neem seed kernel extract (NE) on the post harvest diseases, pathogens of Monilinia fructicola, Penicillium expansum, Trichothecium roseum and Alternaria alternate isolated from the infected fruit were treated with NE in vitro. Results showed that growth of the four pathogens could be significantly (P < 0.05) reduced by NE. The diseases in fruit of plum (Prunus salicina) or Yali pear (Pyrus bertschneideri) inoculated with the pathogens could be prevented remarkably by treated fruit…