Advanced Journal of Microbiology Research

Abstract: In our study, we aimed to determine the validity of diagnostic methods for tuberculosis. Eighty-one people suspected to have tuberculosis were included in the study. The validity of the applied methods for the diagnosis of tuberculosis tuberculin skin test (TST), sputum smear, and used in diagnostic chest X-ray findings (CXR), clinical features and history were evaluated as culture was considered the reference test. Included in the study of 81 people (54 males 27 females) mean age was determined as 45.04 ± 18.69. T…

Abstract: A total of 94 clinical isolates were collected from Gazi University Hospital, Turkey. Presence of ESBL positivity was detected using the double disk synergy test (DDST). ESBL isolates were further typed for the blaTEM, blaSHV, blaCTX-M and blaOXA using designed primers. ESBLs were found in 65 (69.14%) isolates using DDST. Plasmid DNAs of potentially ESBL positive strains were isolated. About 7.69% of the ESBL positive isolates did not harbour plasmid DNA. According to the PCR technique, only 2 additional isolates w…

Abstract: Methicillin-resistant staphylococcus aureus (MRSA) infection has now become a major public health concern. The aim of this study is to determine the antibiotic resistance pattern and prevalence of different staphylococcal cassette chromosome mec (SCCmec) types among the MRSA isolates from a teaching hospital in Shantou, China. The minimum inhibitory concentrations (MICs) of seventeen antimicrobial agents against MRSA isolates were determined using the micro broth dilution method. SCCmec types were identified by mul…

Abstract: Atherosclerosis and heart diseases are major causes of morbidity and mortality in adults in industrialized nations. The aim of this study was to assess the potential influence of high-cholesterol diet-induced oxidative stress on composition and properties of red blood cells (RBCs) in rabbits. Thus, percentage of hematocrit, RBCs, white blood cells (WBCs) and platelets counts, total cholesterol (TC), low density lipoprotein (LDL), triglycerides (TG) and high density lipoprotein (HDL), Thiobarbituric acid reactive su…

Abstract: Different parts of Piliostigma thonningii Scum (Caesalpinioideae) have been used medicinally. The roots and twigs have been used locally in the treatment of dysentery, fever, respiratory ailments, snake bites, hookworm and skin infections in Eastern Nigeria. The leaf extracts has been used for various ethnomedicinal purposes including the treatment of malaria all over Eastern Nigeria. In this study, we have investigated the inhibitory effects of the crude ethanol and methanol extracts for in vitro antimalarial acti…

Abstract: The aim of this study is to transport to dairy plants the making technique of Karin Kaymagi cheese and to make them hygienic cheese. Also, the effects of different packaging materials (artificial case, barrel and tripe) on microorganism counts in the cheese samples were determined. In this research, four different Karin Kaymagi cheese samples were prepared from white cheese, civil cheese, whey cheese, pasteurized cream and concentrated yoghurt mixes. These mixes were put in three different packaging materials (arti…

Abstract: The therapeutic failure of antimycoplasma drugs, subject to resistance is vital for specific microbiological investigation to overcome urogenital diseases among women. The susceptibility of 5 Mycoplasma hominis strains and 11 strains of Ureaplasma urealyticum were studied against six antibiotics namely: minocycline, lincomycin, erythromycin, tetracycline, doxycycline and rosaramicin. Both M. hominis and Ureaplasmas strains were highly resistant to tetracycline. It was observed that both rosaramicin and minocycline…

Abstract: Potentilla fulgens L., an ethno-medicinal plant of the ethnic tribes of Meghalaya in the Eastern Himalayan range of India was screened for root fungal endophytes. This plant is distributed in the wild habitats and in the traditionally preserved ‘Sacred Groves’ of Meghalaya in Eastern India, which is of late getting over-exploited for its medicinal value. Isolation of endophytes in water agar plates yielded a unique fungus that had similar morphological characteristics with the members of the genus Talaromyces. Scan…

Abstract: The antimicrobial activity of DMSO extracts of three bryophyte species, two mosses and a liverwort (Atrichum undulatum (Hedw.) P. Beauv., Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier, Physcomitrella patens (Hedw.) Bruch and Schimp.) grown in nature and in axenic culture was evaluated by microdilution method against eight bacterial species ( Escherichia coli ATCC 35210, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Enterobacter cloacae (human isolate), Listeria monocytogenes…

Abstract: The examination of the gills of 960 fishes belonging to four species: Diplodus annularis, Pagellus erythrinus, Lithognatus mormyrus and Mullus barbatus collected from the Gulf of Annaba, enabled us to identify 13 species of parasitic copepods: Caligus ligusticus, Caligus diaphanus, Clavellotis sp, Clavellotis sargi, Clavellotis pagri, Clavellotis strumosa, Lernaeolophus sultanus, Hatschekia pagellibogneravei, Hatschekia sp, Hatschekia mulli, Sparidicola lithognathi, Neobrachiella exigua and Alella macrotrachelus. T…

Abstract: The aim of this study was to apply the Restriction Fragment Length Polymorphism (RFLP) of Glyceraldehyde-3-Phosphate Dehydrogenase encoding gene (gap) for testing its performance as a molecular typing tool in coagulase negative staphylococci (CNS) isolates from bovine mastitis (n = 59) and human clinical cases (n = 13) identified with VITEK 2. According to the phenotypic identification results, bovine mastitis isolates were Staphylococcus haemolyticus, Staphylococcus simulans, Staphylococcus auricularis, Staphyloco…

Abstract: This study was aimed at investigating: (i) three adhesin genes (clf A, fnb A and cna) in Staphylococus aureus strains, (ii) the presence of slime (ica A and ica D genes) in both Staphylococus epidermidis and S. aureus strains isolated from surgical wounds. The slime and adhesin genes were detected by multiplex PCR. The ica A/ica D positivity rates were determined as 66.2% (104/157) in a total of 157 staphylococcal strains. While the occurance rate of slime genes was 69.6% (48/69) among the S. epidermidis, this rati…

Abstract: We revisited the systematic position of the fungus Moesziomyces penicillariae, the causal agent of smut of pearl millet, by using morphological characters, germination pattern of teliospores and molecular analysis of ribosomal sequences. Samples of smutted ears of pearl millet were harvested in Senegal (West Africa). Compared to the description of Moesziomyces genus sensus Vánky, our samples presented morphological differences: i) presence of a columella-like structure in sori; ii) surface ornamentations of teliosp…

Abstract: The world is facing problems of fossil fuels depletion. The problem of using fossil fuels can have an environmental impact due to the oxidation of CO2, SO2 and NOX emission. In order to overcome those problems, there is an alternative fuel that can replace fossil fuel such as bioethanol and biodiesel. Bioethanol can be derived from biomass with many different varieties of feedstocks such as corn, sugarcane, wood and fruits wastes that are renewable and sustainable resources, which are easily accessible and reliable…

Abstract: The study reports the diversity of emulsifying bacteria in produce water obtained from Bonny Oil Terminal, Niger Delta, Nigeria. Physico–chemical analysis of produce water showed a high chloride ion content of 650.0 mg/L. Total aerobic mesophilic bacterial count was 8.6 x 106 CFU/mL while the oil utilizing bacterial count was 1.32 x 106 CFU/mL. The predominant bacteria genera were Bacillus, Pseudomonas, Serratia and Klebsiella. The ratio of the surface active agent producers in the mesophilic bacteria was 0.34%, wh…