Advanced Journal of Microbiology Research

Abstract: Water samples and sediments were collected from waterways in between two back water lakes namely Vambanad and Asthamudi, in Alukadavu, Kollam along the west coast of Kerala, India. 20 fungal species were isolated and identified by lacto phenol cotton blue staining method. From this, Aspergillus flavus was selected for starch hydrolysis Agar Medium for amylase activity under submerged fermentation for amylase production. The effect of different carbon source, nitrogen source, heavy metals, amino acids, dry biomass w…

Abstract: The aim of this study was to isolate plum-seeds degrading bacterium from plum-grove soil. Some bacteria utilizing plum-seeds were isolated from the plum-grove soil samples by biodegradation assay. Among them, five bacteria had the activity of cellulase and xylanase. Four of the strains grew in the medium containing xylan instead of glucose. Partial sequencing of 16S rRNA and genome indicated that all the isolates belong to the Psedomonas section but two bacteria are unknown. It is proposed that some Pseudomonas bac…

Abstract: The present study focused on the decolorization of textile dye Reactive Black- 5 by actively growing mycelium of fungus Penicillium chrysogenum MT-6, which was isolated from the cement-contaminated soil. Dye decolorization was probably associated with fungal growth and hyphal uptake mechanism (Biosorption/Bioadsorption) . Dye uptake was strongly depended on mycelial morphology. Small uniform pellets with 2 mm size and nutrient-poor medium were found to be better for dye uptake. Optimal conditions for dye uptake by…

Abstract: The antimicrobial activity was determined using the single disc diffusion method. The hexane, methanol and ethyl acetate extracts were assessed for antimicrobial activity against 13 bacteria and a yeast-like fungus, Candida albicans. While flower extracts of Centaurea cankiriense showed significant antibacterial activity against tested strains, the susceptibility of the test microorganisms was less pronounced in the cases of the stem extracts. Hexane extracts from both flower and stem did not show any antibacterial…

Abstract: In this study, we aimed to investigate the effects of boron on in vivo oral yeast colonization in study groups which are exposed to boron dust in different sections of the boron mine. The study was carried out in the boron mining areas of two districts (Eskisehir and Balikesir) of Turkey. We included 184 people working in open quarry and stone milling unit, 144 people working in the factory and 150 people as control group. Specimens were taken from four oral mucosal regions and cultured onto Sabouraud dextrose agar…

Abstract: The increasing clinical bacterial strains resistant to conventional antibiotics have being a great challenge to the public’s health. As a novel kind of antimicrobial agent, defensins are undoubtedly worthy of exploitation for the treatment of antibiotic-resistant bacteria. To evaluate the antibacterial potency of recombinant mature human -defensin 5 (rmHD5) against clinical pathogenic strains, we examined its antibacterial kinetics and bactericidal efficacy on forty-nine bacterial strains (belonging to eleven speci…

Abstract: In this study, ten species of Anabaena were used to test the congruence between the traditional morphological classification system and the present molecular classification system. The electrophoretic patterns for 10 cyanobacterial strains belonging to the genera (Anabaena) were used for molecular analysis using the repeated PCR technique. Genetic diversity was assessed using the banding patterns of repetitive DNA sequences including the HIP1 sequences, enterobacterial repetitive intergenic consensus [ERIC] sequenc…

Abstract: The EF-37 isolate, one of DSE fungi, is beneficial to the growth and development of its host plant, Saussurea involucrata Kar. et Kir. The cultivation requirements including basic culture medium, temperature, light, pH, carbon source and nitrogen compounds were studied for their effects on mycelial growth of a dark-septate endophytic (DSE) fungus EF-37 by using one-factor-at-a- time method. Potato dextrose agar (PDA) was the best medium for the growth of endophyte EF-37. Our studies showed that 20°C, 24 h dark cult…

Abstract: Although Influenza virus infections threaten thousands of lives each year worldwide and bear the risk of been an epidemic, little is known about the circulating strains in Saudi Arabia. The availability of a rapid and accurate diagnostic approach is essential for effective treatment and disease control. In the current study, we optimized a duplex RT-PCR assay for the simultaneous detection of influenza A and B viruses in clinical samples. The developed assay was utilized for testing 100 nasopharyngeal aspirates col…

Abstract: Escherichia coli O157:H7 is a serious and common human pathogen that can cause diarrhoea, haemorrhagic colitis, and haemolytic uraemic syndrome (HUS). In this study, the synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates probe specific to E. coli O157:H7 were performed, and the preparation of colloidal gold immunochromatographic strip based the biotin-streptavidin system was developed for detection of E. coli O157:H7.Monodispersional nanogold colloid was synthesized and…

Abstract: Antibacterial therapeutic failure due to emergence of resistant bacterial strain is a worldwide phenomenon. The search for effective antibacterial substances from sources such as plants has become a necessity to overcome emergent of bacterial resistant in clinical practice. The dried leaves and stem barks of Ficus sycomorus and Ficus platyphylla were collected in Samaru-Zaria, Nigeria in July 2006 and extracted with 70% aqueous ethanol at room temperature. The antibacterial activities such as susceptibility, Minimu…

Abstract: The aim of this study was to evaluate the prevalence rate and antibiotic resistant pattern of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE). A prospective study was conducted at Holy Family Hospital Rawalpindi, Pakistan and Microbiology Research Laboratory, Quaid-i-Azam University, Islamabad, Pakistan during the period from December 2007 to August 2008. The antibiotic resistance pattern was studied for MRSA and MRSE isolated from nasal samples from pa…

Abstract: The purpose of this study was to investigate the infectious pathways used by Streptococcus suis type 2 and enterohemorrhagic Escherchia coli O26 (EHEC O26) in mice. Six-week mice were intraperitoneally injected with mitomycin (0.2 mg/mouse) to decrease the protective immunity. The mice were then challenged with S. suis type 2 and EHEC O26 strains through wounded skin, stomach lavaging and intraperitoneal injection. Enterococcus faecalis ATCC 29212 and E. coli ATCC25922 were used as negative controls. Clinical and m…

Abstract: The increasing clinical bacterial strains resistant to conventional antibiotics have being a great challenge to the public’s health. As a novel kind of antimicrobial agent, defensins are undoubtedly worthy of exploitation for the treatment of antibiotic-resistant bacteria. To evaluate the antibacterial potency of recombinant mature human -defensin 5 (rmHD5) against clinical pathogenic strains, we examined its antibacterial kinetics and bactericidal efficacy on forty-nine bacterial strains (belonging to eleven speci…

Abstract: The objective of this research focused on evaluation of membrane filters application for isolation of Leptospira from surface water samples. The filter materials evaluated included nitrocellulose (0.22 and 0.45 µm pore diameters), polyvinylidene fluoride (Durapore 0.22 and 0.40 m pore diameters), nylon mesh (37 m), and glass fiber (1.0 µm). Millipore polyvinylidene fluoride filter (0.22 m) was examined by scanning electron microscopy to verify that leptospires were present following filtration. Our results suggest…