Advanced Journal of Microbiology Research

Abstract: Random population studies are very important to estimate the environmental factors in nutritional, physiological and breeding experiments. Studying the quantitative traits, consisting of genetic, environmental factors and interaction between them is very important as well. In a random population study, the genetic variation for 50 chicken individuals (Mandarah strain) of three successive generations was examined using differential display technique. The difference between the generations was measured by the change…

Abstract: The study investigated the status of lead and selected mineral elements (copper, zinc and chromium) in type-2 diabetes mellitus patients, so as to establish the possible interactive connections of these metals with the disease and with each other in diabetic patients. Thirty-one patients (15 male and 16 female), clinically diagnosed for type-2 Diabetes mellitus at Sacred Heart Hospital, Abeokuta, Nigeria, participated in the study. Twenty-six apparently healthy individuals (14 male and 12 female) served as control…

Abstract: The importance of -mannosidases in glycoproteins processing and their application in food and pharmaceutical industry led us to further explore plant -mannosidases. Thus, two -mannosidases were purified from matured breadfruit (Artocarpus communis) seeds, by successive chromatography on Diethylaminoethyl-Sepharose CL-6B and Sephacryl S-200 HR to apparent homogeneity. The two isoenzymes named Ma and Mb had native molecular weights of approximately 75 and 60 kDa, respectively. Sodium Dodecyl Sulfate- polyacrylamide g…

Abstract: This study was carried out to determine the blood glucose response and glycemic index of food for diabetics and non-diabetics. The subjects comprised of 10 diabetics and 7 non-diabetics. The results of the physical characteristics showed that the diabetic subjects were on the average older (age bracket between 51 and 70years) than the non-diabetics (18 and 23years) . The Body mass index (BMI) values indicated that over 90% of the diabetics were obese while the non-diabetics were on the average normal. Results also…

Abstract: Biochemical investigations were carried out to assess the effect of consumption of crude oil contami-nated catfish on the hepatocytes and performance of rat. Catfish (Clarias gariepinus) (n = 120) were grouped into 6 of 20 catfish each and held for 30 h in 5 different mixtures of crude oil polluted water (0.1, 0.25, 0.5, 0.75 and 1% v/v). Catfish in the control group were held in borehole water. At the expira-tion of 30 h, the catfish were harvested and used to formulate diet. Albino rats (n = 60) were grouped into…

Abstract: Theoretical suggestion that the metabolism and plasma levels of some hexoses (glucose and fructose) in sickle cell anaemia could be altered was investigated. The levels were determined for 35 normal (HbAA) and for the three sickle cell states (32 HbAS; 35 HbSS, and 33 HbSS-in-crisis, HbSSc). The enzymatic glucose-oxidase method was employed in glucose estimation. The reaction of fructose with indole-3-acetic acid to yield a coloured product was exploited in the estimation of fructose. Mean plasma glucose was found…

Abstract: The effect of Garcinia kola extract on cisplatin-induced renal insufficiency in rats was studied. A total of fifteen rats were used for the study and were split into three groups of five rats per group. Rats in group I were normal controls. Renal insufficiency was induced in rats of groups II and III by a single intraperitoneal administration of cisplatin (5 mg/kg b.w.). After three days, rats in group III received a daily dose of 10 mg/kg b.w. of the extract orally for another seven days. Renal insufficiency was l…

Abstract: The effects of glucose, crystalline cellulose and sawdust of Mitragyna cilata on the growth and cellulase production, inferred from cellulase (EC 3. 2. 1. 4) activity, of Penicillium chrysogenum PCL501 was determined. Glucose-containing media gave the highest mycelia weight of 1.78 mg mL-1 in 120 h of incubation. This is about 3.5 – 4.5 times the maximum weights of 0.51 and 0.40 mg mL-1 respectively obtained from the cultures containing cellulose and sawdust. The cultures containing crystalline cellulose and sawdus…

Abstract: Sweet potato varieties: Nsp, Tz and Spk obtained from Uganda were used in this study. Several phenolic compounds were identified on the basis of their mass spectra in full scan mode and the pattern of their fragmentation. The major compounds found were caffeoylquinic acid, dicaffeoylquinic acid, coumaroylquinic acid, feruloylquinic acid and caffeoylferuloylquinic acids. This is the first time that caffeoylferuloylquinic isomers have been identified in sweet potato samples.[...]

Abstract: Dermatoglyphic analysis of 90 Sickle-cell Anaemia cases and 90 normal subjects was carried out in this study. It involved the digital patterns, ATD angle, A – B ridge count, axial triradius, digital triradius and palmar crease on the hands. 58.44% of the digital patterns in sickle-cell cases were ulnar loop as against 60.14% in the normals. The percentage of Whorl, arch and radial loop in Sickle-Cell group were 31.64%, 8.23% and 2.98% respectively as against 27.47%, 10.43% and 1.98% in the normals. The mean ATD val…