Advanced Journal of Microbiology Research

Abstract: A pot experiment was carried out in a nursery to determine the influence of an arbuscular mycorrhizal (AM) fungus on plant growth and nutrient uptake in tamarind (Tamarindus indica) and jujube (Ziziphus mauritiana), both of which are multipurpose fruit trees in Sahelian agroforestry systems. Plants were inoculated or not with Glomus aggregatum and grown for four months in a sterilized phosphorus (P)-deficient sandy soil (2.18 ppm P) . Tamarind and jujube seedlings grew very poorly in the absence of G. aggregatum, w…

Abstract: The use of the cytochrome oxidase test reagent, as a differential agent to study the pathogenic and saprophytic leptospires was studied. Growth of the leptospiral strains of the saprophytic group was almost involved in the conversion of colorless tetramethyl p-phenelene diamine dihydrochloride solution to dark brown color whereas pathogenic leptospirae strains showed reddish brown color. Saprophytic strains required 20-35 min for oxidation of cytochrome, however, pathogenic strains required more time, about 45 min…

Abstract: Pseudomonas aeruginosa is commonly found in chronic lung infections such as cystic fibrosis and others. Intrinsic antibiotic resistance of P. aeruginosa accompanied by its ability to acquire resistance via mutations and adapt to the heterogeneous and dynamic environment of chronically infected lungs are major threats and reasons for the ultimate failure of the current antibiotic therapies in eradicating the infection from lungs. New insights at molecular levels in the process of accumulating such beneficial mutatio…

Abstract: 30 samples (male and female) of ear swab were collected from patients at general hospital Minna for examination of bacteria that causes otitis media. 26 samples were positive for these organisms. The organisms isolated include Pseudomonas sp., Kleblesella sp., Proteus sp., Escherichia coli and Staphylococcus aureus. Pseudomonas sp., (54%) had the highest incidence of occurrence followed by Kleblesella sp. (19%) and Proteus sp (15%) while the least was S. aureus (4%). The organisms’ sensitivity test reveals that the…

Abstract: Bacillus strains isolated from different habitats were screened and identified for high protease activity. Two of the bacillus strains genetically identified as Bacillus subtilis AG-1 and Bacillus subtilis EAG-2 exhibited tremendous potential for protease production. The strains were optimized with respect to production media and its components. Culture conditions were critically investigated with reference to other key determinants, involving inoculum concentration, incubation time, initial pH of the medium and te…

Abstract: Three fungal strains; namely, Aspergillus terreus, Penicillium janthinellum and Penicillium duclauxii were cultured on different growth media including yeast extract, malt extract, yeast-malt extract, Czapek's Dox, Sabourod's, Harrlod's, and potato dextrose. The growth and secondary metabolites of the three fungal strains were greatly affected by the growth medium. The colour of the culture and secondary metabolites were noticeably altered and changed according to the growth medium used.[...]

Abstract: Bacteriocin producing Lactobacillus plantarum strain isolated from marine shrimp (Penaeus monodon) gut, showed broad range of antibacterial activity against some major food born pathogens. Maximum bacteriocin production was observed at 50°C, pH 4 and 0.9% sodium chloride solution. The bacteriocin has purified by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was pure protein moiety and the molecular weight was 2.5 KDa based on the plasmid curing experiment, suggesti…

Abstract: A greenhouse experiment was conducted to evaluate the effects of different indigenous arbuscular mycorrhizal (AM) fungi on the mobilization of phosphorus from Senegalese natural rock phosphate (NRP) for growth of Gliricidia sepium and Sesbania sesban seedlings. Levels of tested NRP were compatible with high AM fungal proliferation but modified pattern of root colonization according to plant cultivar and fungal species. NRP applications and AM inoculation positively stimulated growth parameters and shoot mineral mas…

Abstract: A study was made of Salmonella contamination in chicken carcasses and giblets sampled from retail outlets in Meknès, Morocco. The serotypes as well as antibiotic-resistance patterns of the Salmonella isolates were determined. A total of 576 samples (144 from popular market, 144 from artisanal slaughterhouses, 144 from poulterers’ shops and 144 from a supermarket) were tested. Among them, 57 (9.90%) were positive for Salmonella, 20.83% (30/57) from popular market, 16.66% (24/57) from artisanal slaughterhouses and 2…