Advanced Journal of Microbiology Research

Abstract: Thirty-four isolates of Colletotrichum spp. including 2 species, C. gloeosporioides and C. capsici, from anthracnose on Bell pepper, Long cayenne pepper and Bird’s eye chilli were isolated and their pathogenicity was proven via fruit inoculation. Pathogenicity tests divided pathogenic potential into low, medium and high virulence groups. It is clearly revealed that C. capsici from the three tested hosts expressed the highest virulent isolates. Cross-inoculation of three high virulent isolates of C. capsici in accor…

Abstract: The traditional processes of sorghum grains malting were investigated in Tamale (Northern Ghana) from the raw materials through to the sun-dried malted sorghum including steeping and germination stages. Samples were taken at each processing stage and cultured for the isolation of LAB. The isolates were characterized by basic phenotyping, ITS-PCR / RFLP analysis and identified by partial sequencing of 16S r RNA genes. Their antimicrobial and amylolytic activities and exopolysaccharides production were also investiga…

Abstract: The intestinal microflora is a positive health asset that crucially influences the normal structural and functional development of the mucosal immune system. In the new born, the colonization of microbes in the alimentary system starts as soon as the womb comes out. A study on the early colonized bacterial species in a new - born will help to understand the immuno protective functions in the new-born. The initial colonization of bacterial population in the alimentary tract of a new-born baby varied according to the…

Abstract: A fungal strain namely, Penicillium steckii was obtained from saline environment of mangrove plant Avicennia marina and evaluated for antifungal activity in PDA against dieback pathogen of rose. Amendments at the level of different carbon and nitrogen source and their concentrations, inorganic and organic salts resultant into the development of new and modified medium of basal PDA under which our fungi exhibited enhanced level of antifungal activity. Achievement of this medium composition may be useful further in p…

Abstract: A total of 672 sera from apparently healthy commercial and indigenous chickens of different ages were screened for antibodies to infectious bronchitis (IB) virus using the enzyme-linked immunosorbent assay (ELISA). Samples obtained from breeder, layer, grower and indigenous chicken flocks in Oyo, Ogun, Ondo and Lagos states of southwestern Nigeria were screened. The total seroprevalence was 82.7% with ELISA units of 77.0 ± 8.0. Among the groups of birds, layers had the highest ELISA units of 80.0 ± 9.0, breeders ha…

Abstract: An investigation was undertaken to determine bacteriological quality of crockery and cutlery of seven restaurants in Federal University of Technology, Yola (FUTY) kiosk. Samples (147) were collected and cultured in appropriate media and the bacterial isolates were identified according to their morphological and biochemical characteristics. The result revealed a profile of seven (7) different bacterial species including Staphylococcus aureus, Proteus vulgaris, Salmonella typhi, Escherichia coli and the species of Sh…

Abstract: In a screen of 175 fungal strains, Aspergillus oryzae LL1 and LL2 were identified as having the highest extracellular leucine aminopeptidase (LAP) activity. LAP activity was optimal when A. oryzae LL1 was subjected to submerged fermentation with an inoculum size of 105 spores per ml and an agitation of 100 rpm at 30°C in media containing defatted soybean and rice husk with a pH of 4.5 for 72 h. Partial characterization of A. oryzae LL1 LAP revealed that it could be greatly inhibited by 2 mmol L-1 of Pb2+, Cu2+, or…

Abstract: The effects of Insulin-Like Growth Factor-1 polymorphism were examined on milk production traits and genetic trends in the Iranian Holstein bulls. A total of 282 bulls were genotyped for SnaBI restriction site in the 5' flanking region of IGF-1 by applying PCR-RFLP method. The allele frequencies for C and T alleles were 0.562 and 0.438, respectively. The results indicated that genetic variants at the 5'-noncoding region of the bovine IGF-1 gene had a marked effect on estimated breeding values of milk (EBVM) and fat…

Abstract: Arbuscular mycorrhizal fungi (AMF) spore population was studied at eight levels of soil depth under two different sites viz., P. cineraria under agro forestry area (with moong bean) and P. cineraria as sole crop at four different quarters of the year (July, October, January, April). In agro forestry areas the spore population recorded higher as compared to P. cineraria grown as sole crop. The upper layer (0-30 cm) contains more number of AMF population in comparison to deeper layers. The trend in decrease of spore…

Abstract: Individual bacteria can accumulate to form biofilm which can help bacteria escape from host immune defense and many antimicrobial agents. Therefore biofilm involves a large number of bacteria which can induce chronic diseases. Here, we compared the differences of biofilm formation among Nontypeable Haemophilus influenzae (NTHi) single culture condition, Pseudomonas aeruginosa (P. aeruginosa) single culture condition and NTHi- P. aeruginosa co -culture condition. Biofilm clone formation unit (CFU) counting revealed…

Abstract: Approximately one third of the world’s population is infected with Mycobacterium tuberculosis and 9.27 million new cases of TB occurred in 2007. Developing countries disproportionately shoulder the global burden of disease with the highest estimated rates in the world, with an estimated 55% of global cases in Asia and 31% in the African region. The incidence of new sputum smear positive in Rwanda through recent national survey was an estimated 162 per 100 000 population. The aim of our study was to evaluate the pre…

Abstract: A field and glass house experiment was conducted with the aim of evaluating Rhizobium inoculation, molybdenum (Mo) and lime supply on growth and nitrogen fixation of nodulated Phaseolus vulgaris. The experiment was laid in a split-split plot design. The experimental treatments consisted of 2 levels of Rhizobium inoculation (with rhizobia and without rhizobia) 3 levels of Mo (0, 6 and 12 g kg-1 of seeds) and 3 levels of lime (0, 2 and 3 t ha-1). Rhizobium inoculation showed significant increase in dry matter yield o…

Abstract: The aim of the present study was to investigate the possible adverse effects of Enterobiasis vermicularis infection on primary school children in Sivas, Turkey, to know whether it affects their school success, mean weight and height or not. Seven primary schools in the central region of Sivas were included in the present study. A total number of 3659 questionnaires were completed by class teachers and parents for each student. In the questionnaire, the height and weight of children, school success and other informa…

Abstract: In recent years, Crimean Congo haemorrhagic fever disease (CCHF) has created a serious health problem in our country. The disease takes place among haemorrhagic viral diseases. This study was made with CCHF diagnosed patients accepted in emergency services to determine the diagnostic criteria and cost effectivness for CCHF. CCHF patients who were accepted in our emergency service between 2004 and 2008 were analyzed retrospectively. Sensitivity, specificity, positive predictive and negative predictive value was calc…

Abstract: The study has been conducted at Tanjung Lumpur, mangrove swamp on January 2009 to isolate and identify the bacterial community in mangrove soil and their resistance against antibiotics. Identified bacteria were Aeromonas hydrophila group 1 and 2, Escherichia coli 1, Chryseomonas luteola, Chromobacterium violaceum, Pseudomonas aeruginosa, Serratia rubudaea, Klebsiella pnuemoniae and Enterobacter cloacae. The identified bacteria were introduced to fourteen different antibiotics to determine the bacterial susceptibili…