Advanced Journal of Microbiology Research

Abstract: Presence of secreted proteins is the key for sustenance of Staphylococcus aureus strains in the host system. General secretory (Sec) system is a well known and major secretion system that is responsible for the transportation of exoproteins across the bacterial cytoplasmic membrane and cell wall. In order to define the secretome of S. aureus, the availability of combination of two-dimensional gel electrophoresis (2-DE) with the latest advanced techniques (MALDI-TOF and ESI-MS/MS) has provided a powerful tool for th…

Abstract: Hepatitis B (HBV) and hepatitis C (HCV) virus infections have remained recurring decimals in blood transfusion, vertical transmission, liver cirrhosis and hepatocellular carcinoma (HCC). Most of the published studies of their prevalence in Northern Nigeria are among HIV/AIDS patients. The need for such vital information among an apparently healthy population was the basis for this study. Rapid diagnostic tests were used to screen for HBsAg and anti-HCV antibodies among people of a local community in Northern Nigeri…

Abstract: Enterobacteria are often responsible for various gastrointestinal foods borne infection in humans especially in the developing countries. Cassia occidentalis, Croton zambensicus and Newbouldia leavis known as ‘Ewe ori esi’, Ajekobale and Akoko respectively in Yoruba are three plants whose leaves are used in combination by boiling to treat gastrointestinal and diarrhea illnesses among indigenes of Akungba-Akoko, Ondo State, Nigeria. The leave extracts from these medicinal plants were screened in vitro in the laborat…

Abstract: The effects of three soil collections from old pine plantations in Nigeria: Enugu, Ugwuoba and Nsukka and a control soil on mycorrhizae development as well as morphology of the mycorrhizal rootlets, anatomical confirmation of mycorrhizae development were investigated. Enugu soil induced the greatest mycorrhizal infection (44%) and growth of seedlings. It is, therefore, recommended for nursery inoculations of pine seedlings. Bifurcate mycorrhizae characteristic of pines were observed in all treatments. Coherent rhiz…

Abstract: Ureaplasma sp. is associated with chronic lung disease and it has been implicated in the morbidity and mortality of new born babies (human). Adherence is an important process for lung infection, and scarce information is available in this respect. This work shows its adhesion to epithelial cells from the respiratory tract in culture, and evaluates the Ureaplasma sp. references and clinical strains isolated from premature newborns. Ureaplasma parvum and Ureaplasma urealyticum serotype 8 and five clinical samples wer…

Abstract: An efficient protocol for protoplast isolation and regeneration was first established in Coprinus Comatus. The highest yield of protoplasts was up to 8.9 × 106 cells/ml in digestion solution containing 2.0% lywallzyme and 0.6 M KCl after incubation of four-day-old mycelia at 30°C for 3 h; among which, about 1.4% protoplasts could be regenerated into mycelia after 4 - 6 days of incubation at 25°C in CYM medium with 0.6 M mannitol as osmotic stabilizer. The results are beneficial for breeding new cultivars by the met…

Abstract: The aim of this study was to investigate the mycoflora of the loggerhead turtle, Caretta caretta, from its nests and eggshells at Fethiye Beach, Turkey. During the 2004 breeding season, following the complete emergence of the hatchlings, sand samples were collected from 15 nests and eggs from these nests were swabbed. Rose Bengal chloramphenicol agar was used for isolation, and then mycoflora were subcultured onto suitable media. Fungi were counted and identified at genus level. Ten genera were identified within th…

Abstract: 12 strains of lipase - producing were isolated from 36 samples collected from oil - contaminated soil of machinery - repair plant, dining room, and vegetable market, respectively, of which strain (3 - 2) had the highest hydrolytic activity (17.1 U/ml). Immobilized cell of strain 3 - 2 onto sodium alginate and immobilized crude enzyme solution of strain (3 - 2) onto Sodium alginate were respectively used in two transesterification programs to transfer the Fatty acid methyl ester (FAME). Results showed that both immo…

Abstract: A novel microbial phospholipase C from Bacillus mycoides strain 970 was purified to apparent homogeneity by an improved purification process, in which DEAE-Cellulose adsorption was first used to remove bulk of dark brown impurities and then a combination of DEAE-Cellulose ion-exchange, Phenyl Sepharose hydrophobic interaction and Sephacryl S-100 gel filtration chromatography was used to separate the enzyme from remained contaminants. The enzyme appeared to be a single peptide of 75.1 kDa on SDS-PAGE. The optimum te…

Abstract: Studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last two decades. The leaves of Hibiscus sabdariffa (red and green leaved) and Celosia argentea were studied for their antiviral activities against Measles Virus (MV) as well as the effects of the extracts on Hep -2 cells. Ethanol extract of the leaves of each of these plants showed no toxicity to the Hep-2 cells at all concentrations used (5, 10 and 15 mg/ml). The pre-inoculative treatment of Hep-2 cells with p…

Abstract: The aim of the present study is to determine the in vitro antimicrobial activity of various extracts, partitions and fractions of Muntingia calabura (Elaeocarpaceae) leaves against a selected panel of microorganisms. The leaves of M. calabura were soaked separately in the aqueous, chloroform and methanol solvent systems in the ratio of 1:20 (w/v) for 72 h and these procedures were repeated three times. Antimicrobial testing was carried out using the micro -broth dilution method. The microbes targeted were Staphyloc…

Abstract: The medium components influencing the enzyme production were screened using Plackett-Burman design. A total of 67 nutrients comprising of fifteen each of carbon, nitrogen, mineral/salt and eleven each of inorganic nitrogen sources and amino acid sources were screened for the production of L-asparaginase by SmF. This design involves screening of up to ‘n-1’ variables in just ‘n’ number of experiments. Regression coefficients and t-values were calculated by subjecting the experimental data to statistical analysis. Am…

Abstract: In this study we assessed the chemical composition, antioxidant and antibacterial activities of Lavender essential oil. The antioxidant and antibacterial capacity of test sample was assayed by a linoleic acid system and conventional method of bacterial growth inhibition. The results demonstrated that the essential oil consisted of 1,5-Dimethyl-1-vinyl-4-hexenyl but yrate as the most abundant component (43.73%), followed by 1,3,7-Octatriene, 3,7-dimethyl- (25.10%), Eucalyptol (7.32%), and Camphor (3.79%). Lavender e…

Abstract: The antibiotic produced by a newly isolated Bacillus subtilis has potent anti-tubercular activity against sixteen strains of Mycobacterium tuberculosis, most of which belong to MDR strains. MICs for the sixteen strains were evaluated. A series of spectrum analysis and element analysis determined that the antibiotic’s molecular formula was C27H46O2.[...]

Abstract: In this experiment, we have used the soil profile as a biological filter that can to absorb pollution and microbes in the domestic wastewater at Iran during 2009. In this experiment, we had 15 lysimeters, that were planted canola (Brassica napus L.) in 1 to 5 lysimeters and were irrigated by domestic wastewater with BOD5 about 150 mg/lit and primary drainage water were accumulated. In the 6 to 9 lysimeters was planted alfalfa (Medicago sativa L.) and were irrigated by primary drainage water and then, were accumulat…