Advanced Journal of Microbiology Research

Abstract: Through biological inoculation technology, the bacterial-mycorrhizal-legume tripartite symbiosis in artificially heavy metal polluted soil was documented and the effects of dual inoculation with arbuscular mycorrhizal (AM) fungus and Rhizobium (N- fixing bacteria, NFB) on the host plant cowpea (Vigna sinensis) in pot cultures were investigated at six concentrations of Zn (0.0 – 1000 mg/kg dry soil) and Cd (0.0 – 100 mg/kg dry soil). From a number of physiological indices measured in this study, microsymbionts signi…

Abstract: The microorganisms involved in dry-yam (gbodo) and water used in parboiling from different source (well, pipe-borne, river, stream and pond) were investigated. There was predominance of Staphylococcus aureus, fungi and coliforms in the gbodo samples. Coliforms were also observed in all the parboiling water. In gbodo, the total viable bacterial count was generally high, ranging from 1.1 x 106 cfu/ml from Oyo processing area to 7.8 x 105 cfu/ml from Iseyin processing areas. A large percentage of the microorganisms in…

Abstract: Present study reveals florigenic effects of growth hormones (i.e. gibberellic acid, GA3) and heavy metals (i.e., Pb(NO3)2 and HgCl2) in Cucumis sativus L. and Momordica charantia L. Applied GA3 at 400 ppm caused precocious flowering, increasing the number of pistillate and staminate flowers in both plants. Application of Pb(NO3) 2 and HgCl2 caused significant delay in flowering, consequently leading to reduction in number of flowers. However, when GA3 was applied with Pb(NO3)2 and HgCl2, there was less decrease in…

Abstract: The objective of the present study is to develop an efficient protocol for shoot and plant regeneration using five commercial canola cultivars grown under the Egyptian agricultural conditions. The regeneration efficiency from hypocotyl explants was examined. The data indicated that embryonic calli were formed within two weeks in the presence of 1 mgl-1 2,4-D. Adventitious shoots emerged from the embryonic callus in the presence of 4.5 mgl-1 BA. The cultivars showed a varied response to shoot regeneration. Regenerat…

Abstract: One hundred stool samples were collected from children and pupils in Akure, Ondo State, Nigeria. These were examined for the presence of enteric bacteria associated with diarrhea. Seventy of the samples were collected from apparently healthy pupils in some public primary schools in Akure, while thirty samples were collected from children in the Children’s Ward of the Ondo State Specialist Hospital, Akure. All the samples were pre-enriched for Salmonella and Shigella in selenite–F broth and also streaked on MacConke…

Abstract: The use of rice and melon husks in their raw, carbonized (at 400°C and 600°C) and activated (using 10%, v/v orthophosphoric acid) form to catalyze the degradation of anthracene in sandy soil was investigated. Rice husk carbonized at 600°C and activated was found to be a better adsorbent than melon husk under same condition. After five weeks of degradation experiment the anthracene reduced from an initial concentration of 5000 to 1009.7 and 1200.2 µg/g soil for rice and melon husks, respectively, while the control r…

Abstract: Anthocyanin is among the permitted pigments that can be used for food colourant and having been considered a potential replacement for synthetic dyes. The objective of this study is to analyse the colour pigment, anthocyanin, that can be detected in flower and their stability in extracted form. All the analysed results will be used in the next study for the production of new food colouring material. From the observation, it shows that S3 flower developmental stage contains the highest anthocyanin concentration. Sim…

Abstract: A laboratory experiment was conducted to study the effect of selected fungicides, tridemorph and captan, at concentrations ranging from 0 to10 kg ha-1 on the activity of invertase and cellulase in a vertisol. The activities of invertase and cellulase were significantly more at tridemorph and captan levels of 2.5 and 5.0 kg ha-1, respectively. But at higher concentrations of 7.5 and 10 kg ha-1respectively, tridemorph and captan were toxic to both cellulase and invertase activities. In soil samples receiving 2.5-5.0…

Abstract: The study applied triphenyl tetrazolium chloride-dehydrogenase activity (TTC-DHA) method to detect the activities of attached biofilm on bio-activated carbon (BAC) samples in the up-flow aerated biological activated carbon filter (UABACF) treating textile secondary effluent. Modification to the conventional TTC-DHA determination method was proposed. In the modification, BAC samples were used directly to measure TTC-DHA without pre-separating the attached biofilm from carbon samples. After modification, the mean val…

Abstract: Suspended cultures of white-rot fungus, Trametes versicolor, supplemented with bagasse powder showed a concentration dependent enhancement in the ligninolytic enzymes activity in liquid shake cultures. 2% (w/v) bagasse powder improved greater stability to the enzymes. The optimum pH is 3.5 and the optimum temperature is 40°C for maximum lignonolytic enzymatic activity. The optimum shaking speed is 60 rpm for maximum enzymatic activity. The maximum enzymatic activity showed by T. versicolor is 495, 440 and 410 mmol/…

Abstract: Germination and/or fermentation processes for Egyptian breeds of mung seeds were carried out with three Lactobacillus strains namely, L. reuteri, L. case, and L. heleviticus. Results revealed increase in protein content, nitrogen solubility and in vitro digestibility for all treated mung meals. Treated mung proteins contained most of the essential amino acids in concentrations comparable to those recommended by the FAO/WHO, with the exception of the sulphur-containing amino acids. Trypsin inhibitor units (TIU) and…

Abstract: The need to develop the best off vine mango ripening technique for both consumption and processing was investigated. Sensory quality and microbial contamination was studied on mature green Dodo mangoes before and during a 3- and 6-day ripening period by Smoked Pit Ripening (SPR), Ethylene (fruit generated) Pit Ripening (EPR), Untreated Pit Ripening (UPR) and Room Temperature Ripening (RTR) as a control method. The post harvest ripening changes in the quality characteristic of ripe mangoes were correlated among trea…

Abstract: Theaflavins is a group of very important material from black tea with functions as antioxidant, cancer suppressor and inhibitor of pathogenic microorgnisms. A discription is given on the property, chemical structure and application of theaflavin in this paper. The present research situation of theaflavins both in China and abroad is also reviewed.[...]

Abstract: Amplified fragment length polymorphism (AFLP) was used to assess genetic diversity and relationships among 15 accessions of Irvingia gabonensis collected from Cameroun, Gabon, and Nigeria. Twelve AFLP+3 primers produced 384 polymorphic fragments. Average genetic distance (AGD) between the 15 accessions was 58.7% (32-88%). AGD and range of genetic distance among accessions from Cameroun, Nigeria and Gabon were 62% (53-76%), 52% (32.3 – 84.8%) and 50% (45-53%), respectively, indicating more genetic diversity in Camer…

Abstract: The distribution and gene frequencies of ABO and rhesus (Rh) blood groups and haemoglobin variants for samples of the Nigerian population at Ogbomoso was determined. Data consisting of records of blood groups and haemoglobin types of different ages ranging from infants to adults for a period of 4 to 6 years (1995 – 2000) was collected from Baptist Medical Centre (BMC), Ladoke Akintola University of Technology Health Centre (LAUTHC) and Oyo State General Hospital (OSGH), all in Ogbomoso, Oyo State, Nigeria. Overall…