Advanced Journal of Microbiology Research

Abstract: An amylolytic yeast strain was isolated from starchy soils (flour mills environment, flour market, cassava farms after harvesting and transformation of tubers) and its enzyme productivity and activity evaluated. The enzyme synthesis was optimum at 30°C when initial pH of fermentation medium was 4.5. After extraction and partial purification, the enzyme remained stable in a range of temperature and pH between 20°C-60°C and 2-8 pH respectively. The optimum enzyme activity was displayed at 70°C and pH 5.5 and 6.5. The…

Abstract: The effects of genetic and non genetic factors on the number of ovarian follicles and oocyte yield and quality in the bovine local breed (Oulmes Zaer), exotic breeds and their crosses in Morocco was investigated. In this study, females in very bad body conditions (BCS < 2) were not slaughtered and the average. The body condition scores (BCS) was 2.94 ± 0.89. Although some individual values were out of the normal ranges, mean values of total proteins, albumin, urea, -OH and GOT remain normal and were 77.83 ± 8.74 g/…

Abstract: Eight Aspergillus niger strains which produced strong starch degrading amylase were isolated from the soil using a medium containing Remazol Brilliant Blue (RBB) starch as substrate. Amylase production was detected by the disappearance of the blue colour around the colony. Among the isolates, A. niger AM07 produced the largest clear zone (7.0mm) on Remazol Brilliant Blue (RBB) agar plate and also gave the highest amylase yield (806 U/ml) in solid-state fermentation process, hence it was selected for further studies…

Abstract: Cellulases are a group of hydrolytic enzymes capable of degrading cellulose to smaller sugar components like glucose units. These enzymes are produced by fungi and bacteria. The aim of this research was to identify a Aspergillus species with over production of endo- -1,4-glucanase. Properties of endo- -1,4-glucanase/carboxymethylcellulase (CMCase) from a culture filtrate of the Aspergillus sp. was also studied. Aspergillus sp. (R4) was selected as over producer of endo- -1,4-glucanase among 13 different species. SD…

Abstract: The potential of various organisms to catabolize and metabolize organic compounds has been recognized as potentially effective means of disposing of hazardous wastes. Phenolic compounds has long been recognized as one of the most recalcitrant and persistent substance in petroleum refinery effluents. This is a cause of some concern because of the high toxicity and of this compound. Bioremediation of phenolic compounds has been recognized as a potential solution for the disposal of phenolic compounds due to its scale…

Abstract: Cowpea Trypsin Inhibitor (CpTI) gene was transferred into cauliflower by agrobacterium-mediated transformation method, and 14 transgenic cauliflower plants were obtained. Cotyledons and hypocotyls were used as explants. The putative transformants were assayed by PCR and Southern blotting analysis. The results indicated that CpTI gene was transferred into cauliflower successfully. The result of preliminary insect-resistant assay showed that the transgenic plants were more resistant to Pieris rapae than non-transgeni…

Abstract: Plants sense the quality, quantity, and duration of light signals and use them to optimise their growth and development. These signals are perceived by special light receptors of which the phytochrome pigment system is one of the most important for photomorphogenetic responses. Using special diodes that emit monochromatic light, we studied the effect of red (R), far-red (FR) and R+FR combinations on hypocotyl elongation of latitudinal ecotypes of Betula pendula. Continuous R and FR inhibited hypocotyl elongation eq…

Abstract: A glasshouse experiment was carried out to study the effect of ammonium -N on the nodulation, growth and N-uptake of Sesbania sesban (L.) Merr. inoculated with an effective Mesorhizobium (S. sesban) strain. Ammonium-N was supplied twice weekly as 100 ml of nutrient solution at concentrations of 0, 100, 200, 400, 600 and 800 mg N/l. The seedlings were grown in pots with sterilized sand substrate and assessed at 5, 7 and 9 weeks after planting (WAP). Nitrogen supply significantly improved nodulation (number of nodule…

Abstract: Transgenic azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] plants expressing the hygromycin phosphotransferase ( hpt), green fluorescent protein (sgfp) and phosphinothricin acetyltransferase (bar) genes were obtained by Agrobacterium- tumefacients - mediated transformation. A total of 210 epicotyl explants were inoculated with A. tumefaciens strain EHA105, harboring the binary plasmid pZHBG on MS co-cultivation medium supplemented with 100 mM acetosyringone and 10 mg/l of BA. Following selection on MS medium wi…

Abstract: To explore the potential for in vitro rapid regeneration of Parkia biglobosa, an endangered multipurpose woody angiosperm, cotyledon explants obtained from 7-day old aseptically germinated seedlings were cultured on Murashige and Skoog (MS) basal medium supplemented with 0.4-1.0 mg/L naphthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Cotyledon explants were placed either with the abaxial surface facing up or down on the media. Induced calli were subcultured on media containing 0.8 mg/L Kinetin…

Abstract: Graded doses of Nature Cure Bitters (NCB) were administered daily (100, 200 and 400 mg/kg p.o) to rats for 28 days and the effects on body weight, organ weight, clinical signs, gross pathology, haematology, histology and serum biochemical parameters were evaluated. The relative weights of the heart, liver and testes of treated rats were unaffected in contrast to a significant increase in the relative weights of the lungs, kidneys and spleen. The packed cell volume and haemoglobin concentrations were significantly r…

Abstract: The pattern of infection and in vivo response of uncomplicated Plasmodium falciparum malaria to Chloroquine as first line drug and Quinine, Halofantrine or Sulfadoxine-Pyrimethamine as second line medications was evaluated at nested sentinel points, including Government and Private Practices, for three consecutive months. 559 cases were evaluated of which 22.5% failed on Chloroquine therapy. The age range of P. falciparum malaria cases was 4 months to 48 years, with a mean and median age of 9.2 and 3 years, respect…

Abstract: Two hundred isolates of Presumed Staphylococcus aureus from routine clinical specimens, collected from two government hospitals in Abha, Saudi Arabia, had their identity verified. We used the tube coagulase test as our gold standard. Twenty (10%) of the isolates were mis-identified. Reliance by the two laboratories on DNase test, Mannitol fermentation reaction and improper performance of the coagulase test, were responsible for the mis-diagnosis.[...]

Abstract: A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were separately incorporated in chitin agar plates. After running polyacrylamide gel electrophoresis, the gel was transferred onto chitin agar plate containing different dyes for the activity staining. Thin layer of acetate buffer (0.2 M, pH 5) was pored on the gel, which hel…

Abstract: In Northern Nigeria, different parts of Nauclea latifolia (family: Rubiaceae) are prescribed as remedy for diabetes mellitus. This study was designed to evaluate the hypoglycemic property of aqueous extract of the leaves of the plant in normal and alloxan-induced diabetic rats. The aqueous extracts at (200 mg/kg bw.) significantly lowered glucose levels (p[...]