By: Adenike Kuku*, Oludele Odekanyin, Kemi Adeniran, Mary Adewusi and Toyin Olonade
Abstract: A lectin was isolated and purified from the pepper seeds Capsicum annuum. The purification procedure involved anionic exchange chromatography on DEAE – Cellulose and QAE – Sephadex columns followed by gel filtration on Sephadex G–100. The heamagglutinating activity of the lectin towards human erythrocytes was sensitive to inhibition by D-mannose and D-glucose; and enhancement by CaCl2 and MgCl2. The lectin activity was enhanced at very high acidic pH, inhibited at high basic pH but stable at physiological pH range…
Keywords: Lectin; pepper; seeds; antifungal; glucose; mannose.
AJMR 2009, 8(1), 1-6;